Recombinant Anti-ALIX antibody [EPR15314] - N-terminal (ab186429)
![Western blot - Anti-ALIX antibody [EPR15314] - N-terminal (ab186429)](https://www.abcam.com/ps/products/186/ab186429/Images/ab186429-219973-anti-alix-antibody-epr15314-n-terminal-western-blot.jpg "Western blot - Anti-ALIX antibody [EPR15314] - N-terminal (ab186429)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15314] to ALIX - N-terminal
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-ALIX antibody [EPR15314] - N-terminal
See all ALIX primary antibodies -
Description
Rabbit monoclonal [EPR15314] to ALIX - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293, HeLa, K562, Jurkat, RAW 264.7, PC12, and NIH 3T3 cell lysates; Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15314 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 97, 80 kDa (predicted molecular weight: 97 kDa).
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 97, 80 kDa (predicted molecular weight: 97 kDa). |
Application notes
Is unsuitable for Flow Cyt.
Target
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Function
Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function implies the interaction with CHMP4B. May play a role in the regulation of both apoptosis and cell proliferation. -
Sequence similarities
Contains 1 BRO1 domain. -
Cellular localization
Cytoplasm > cytosol. Melanosome. Cytoplasm > cytoskeleton > centrosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Colocalized with CEP55 in the midbody during cytokinesis. Colocalized with CEP55 at centrosomes of non-dividing cells. - Information by UniProt
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Database links
- Entrez Gene: 10015 Human
- Entrez Gene: 18571 Mouse
- Entrez Gene: 501083 Rat
- Omim: 608074 Human
- SwissProt: Q8WUM4 Human
- SwissProt: Q9WU78 Mouse
- SwissProt: Q9QZA2 Rat
- Unigene: 475896 Human
see all -
Alternative names
- AIP1 antibody
- ALG 2 interacting protein 1 antibody
- ALG-2-interacting protein 1 antibody
see all
Images
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Western blot - Anti-ALIX antibody [EPR15314] - N-terminal (ab186429)All lanes : Anti-ALIX antibody [EPR15314] - N-terminal (ab186429) at 1/5000 dilution
Lane 1 : C6 cell lysate
Lane 2 : RAW 264.7 cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 97 kDa -
![Western blot - Anti-ALIX antibody [EPR15314] - N-terminal (ab186429)](https://www.abcam.com/ps/products/186/ab186429/Images/ab186429-219972-anti-alix-antibody-epr15314-n-terminal-western-blot.jpg)
Western blot - Anti-ALIX antibody [EPR15314] - N-terminal (ab186429)All lanes : Anti-ALIX antibody [EPR15314] - N-terminal (ab186429) at 1/5000 dilution
Lane 1 : 293 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 97 kDa -
![Anti-ALIX antibody [EPR15314] - N-terminal (ab186429)](https://www.abcam.com/ps/products/186/ab186429/Images/ab186429-4-benefits-of-recombinant-antibodies.png)
Anti-ALIX antibody [EPR15314] - N-terminal (ab186429)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (52)
ab186429 has been referenced in 52 publications.
- Kim H & Shin S ExoCAS-2: Rapid and Pure Isolation of Exosomes by Anionic Exchange Using Magnetic Beads. Biomedicines 9:N/A (2021). PubMed: 33401715
- Matejovic A et al. Comparison of separation methods for tissue-derived extracellular vesicles in the liver, heart, and skeletal muscle. FEBS Open Bio 11:482-493 (2021). PubMed: 33410274
- Zhang Y et al. Phosphatase Shp2 regulates biogenesis of small extracellular vesicles by dephosphorylating Syntenin. J Extracell Vesicles 10:e12078 (2021). PubMed: 33732417
- Liu L et al. MicroRNA-15a Carried by Mesenchymal Stem Cell-Derived Extracellular Vesicles Inhibits the Immune Evasion of Colorectal Cancer Cells by Regulating the KDM4B/HOXC4/PD-L1 Axis. Front Cell Dev Biol 9:629893 (2021). PubMed: 33732698
- Zhang K et al. Nuclear exosome HMGB3 secreted by nasopharyngeal carcinoma cells promotes tumour metastasis by inducing angiogenesis. Cell Death Dis 12:554 (2021). PubMed: 34050127