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RecombinantRabMAb

Recombinant Anti-ALIX antibody [EPR15314] - N-terminal (ab186429)

Reviews (3) Submit a question References (69)
Western blot - Anti-ALIX antibody [EPR15314] - N-terminal (ab186429)
  • Western blot - Anti-ALIX antibody [EPR15314] - N-terminal (ab186429)
  • Anti-ALIX antibody [EPR15314] - N-terminal (ab186429)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15314] to ALIX - N-terminal
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-ALIX antibody [EPR15314] - N-terminal
    See all ALIX primary antibodies

  • Description

    Rabbit monoclonal [EPR15314] to ALIX - N-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293, HeLa, K562, Jurkat, RAW 264.7, PC12, and NIH 3T3 cell lysates; Jurkat cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab186429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/1000 - 1/10000. Detects a band of approximately 97, 80 kDa (predicted molecular weight: 97 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 97, 80 kDa (predicted molecular weight: 97 kDa).
Application notes
Is unsuitable for Flow Cyt.

Target

  • Function

    Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function implies the interaction with CHMP4B. May play a role in the regulation of both apoptosis and cell proliferation.

  • Sequence similarities

    Contains 1 BRO1 domain.

  • Cellular localization

    Cytoplasm > cytosol. Melanosome. Cytoplasm > cytoskeleton > centrosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Colocalized with CEP55 in the midbody during cytokinesis. Colocalized with CEP55 at centrosomes of non-dividing cells.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AIP1 antibody
    • ALG 2 interacting protein 1 antibody
    • ALG-2-interacting protein 1 antibody
    • ALG2 interacting protein X antibody
    • Alix antibody
    • Apoptosis linked gene 2 interacting protein X antibody
    • Dopamine receptor interacting protein 4 antibody
    • DRIP4 antibody
    • Hp95 antibody
    • KIAA1375 antibody
    • MGC17003 antibody
    • PDC6I_HUMAN antibody
    • PDCD6 interacting protein antibody
    • PDCD6-interacting protein antibody
    • PDCD6IP antibody
    • Programmed cell death 6 interacting protein antibody
    • Programmed cell death 6-interacting protein antibody
    see all

Images

  • Western blot - Anti-ALIX antibody [EPR15314] - N-terminal (ab186429)
    Western blot - Anti-ALIX antibody [EPR15314] - N-terminal (ab186429)
    All lanes : Anti-ALIX antibody [EPR15314] - N-terminal (ab186429) at 1/5000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW 264.7 cell lysate
    Lane 3 : PC12 cell lysate
    Lane 4 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 97 kDa

  • Western blot - Anti-ALIX antibody [EPR15314] - N-terminal (ab186429)
    Western blot - Anti-ALIX antibody [EPR15314] - N-terminal (ab186429)
    All lanes : Anti-ALIX antibody [EPR15314] - N-terminal (ab186429) at 1/5000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 97 kDa

  • Anti-ALIX antibody [EPR15314] - N-terminal (ab186429)
    Anti-ALIX antibody [EPR15314] - N-terminal (ab186429)

References (69)

Publishing research using ab186429? Please let us know so that we can cite the reference in this datasheet.

ab186429 has been referenced in 69 publications.

  • Born LJ  et al. Sustained released of bioactive mesenchymal stromal cell-derived extracellular vesicles from 3D-printed gelatin methacrylate hydrogels. J Biomed Mater Res A 110:1190-1198 (2022). PubMed: 35080115
  • Wang Z  et al. Characterization of Bovine Foamy Virus Gag Late Assembly Domain Motifs and Their Role in Recruiting ESCRT for Budding. Viruses 14:N/A (2022). PubMed: 35336929
  • Duan S  et al. Peripheral Serum Exosomes Isolated from Patients with Acute Myocardial Infarction Promote Endothelial Cell Angiogenesis via the miR-126-3p/TSC1/mTORC1/HIF-1α Pathway. Int J Nanomedicine 17:1577-1592 (2022). PubMed: 35400999
  • Sun P  et al. Extracellular vesicle-packaged mitochondrial disturbing miRNA exacerbates cardiac injury during acute myocardial infarction. Clin Transl Med 12:e779 (2022). PubMed: 35452193
  • Bonhoure A  et al. Extracellular 20S proteasome secreted via microvesicles can degrade poorly folded proteins and inhibit Galectin-3 agglutination activity. Traffic 23:287-304 (2022). PubMed: 35466519
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Western blot abreview for Anti-ALIX antibody [EPR15314] - N-terminal

 Average
Abreviews
abreview image
Application
Western blot
Sample
Rabbit Cell lysate - whole cell (SH-SY5Y)
Gel Running Conditions
Reduced Denaturing (4-12% Bris-Tris)
Loading amount
75000 cells
Specification
SH-SY5Y
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 16 2022

Immunocytochemistry abreview for Anti-ALIX antibody [EPR15314] - N-terminal

 Good
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Rabbit Cell (SH-SY5Y)
Permeabilization
Yes - Permeabilised in PBS/0.25% (v/v) Triton X-100 for 2 minutes at RT
Specification
SH-SY5Y
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 03 2022

Western blot abreview for Anti-ALIX antibody [EPR15314] - N-terminal

 Inconclusive
Abreviews
abreview image
Application
Western blot
Sample
Bos taurus Purified protein (Extracellular vesicle (EV) enriched whey fraction)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris gel)
Loading amount
20 µg
Specification
Extracellular vesicle (EV) enriched whey fraction
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 27 2020

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