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RecombinantRabMAb

Recombinant Anti-ALIX antibody [EPR23653-13] - BSA and Azide free (Capture) (ab277682)

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Sandwich ELISA - Anti-ALIX antibody [EPR23653-13] - BSA and Azide free (Capture) (ab277682)

    Key features and details

    • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
    • Rabbit monoclonal [EPR23653-13] to ALIX - BSA and Azide free (Capture)
    • Suitable for: Sandwich ELISA
    • Reacts with: Human

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    Overview

    • Product name

      Anti-ALIX antibody [EPR23653-13] - BSA and Azide free (Capture)
      See all ALIX primary antibodies
    • Description

      Rabbit monoclonal [EPR23653-13] to ALIX - BSA and Azide free (Capture)
    • Host species

      Rabbit
    • Tested applications

      Suitable for: Sandwich ELISAmore details
    • Species reactivity

      Reacts with: Human
    • General notes

      ab277682 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human ALIX. The recommended pair for sandwich ELISA is:
           Capture: ab277682, Human ALIX Capture Antibody (unconjugated)
           Detector: ab277681, Human ALIX Detector Antibody (unconjugated)
      The reference range value is 0.625 - 40,000 pg/mL.

      Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

      This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

      Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

      The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free production
      For more information see here.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      Constituent: 100% PBS
    • Carrier free

      Yes
    • Concentration information loading...
    • Clonality

      Monoclonal
    • Clone number

      EPR23653-13
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Apoptosis
      • Extracellular Signals
      • Death Ligands
      • Microbiology
      • Interspecies Interaction
      • Host Virus Interaction
      • Signal Transduction
      • Protein Trafficking
      • Vesicle Transport
      • Regulation

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
      • Goat Anti-Rabbit IgG H&L (Biotin) (ab207995)
      • Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)
    • Conjugation kits

      • Alkaline phosphatase Conjugation Kit - Lightning-Link® (ab102850)
      • HRP Conjugation Kit - Lightning-Link® (ab102890)
      • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
    • sELISA pair antibody

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • Streptavidin Conjugation Kit - Lightning-Link® (ab102921)
      • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
      • Anti-ALIX antibody [EPR23653-32] - BSA and Azide free (Detector) (ab277681)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab277682 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA
    Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR23653-32] to ALIX - BSA and Azide free (Detector) (ab277681).
    Notes
    Sandwich ELISA
    Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR23653-32] to ALIX - BSA and Azide free (Detector) (ab277681).

    Target

    • Function

      Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function implies the interaction with CHMP4B. May play a role in the regulation of both apoptosis and cell proliferation.
    • Sequence similarities

      Contains 1 BRO1 domain.
    • Cellular localization

      Cytoplasm > cytosol. Melanosome. Cytoplasm > cytoskeleton > centrosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Colocalized with CEP55 in the midbody during cytokinesis. Colocalized with CEP55 at centrosomes of non-dividing cells.
    • Target information above from: UniProt accession Q8WUM4 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 10015 Human
      • Omim: 608074 Human
      • SwissProt: Q8WUM4 Human
      • Unigene: 475896 Human
      • Alternative names

        • AIP1 antibody
        • ALG 2 interacting protein 1 antibody
        • ALG-2-interacting protein 1 antibody
        • ALG2 interacting protein X antibody
        • Alix antibody
        • Apoptosis linked gene 2 interacting protein X antibody
        • Dopamine receptor interacting protein 4 antibody
        • DRIP4 antibody
        • Hp95 antibody
        • KIAA1375 antibody
        • MGC17003 antibody
        • PDC6I_HUMAN antibody
        • PDCD6 interacting protein antibody
        • PDCD6-interacting protein antibody
        • PDCD6IP antibody
        • Programmed cell death 6 interacting protein antibody
        • Programmed cell death 6-interacting protein antibody
        see all

      Images

      • Sandwich ELISA - Anti-ALIX antibody [EPR23653-13] - BSA and Azide free (Capture) (ab277682)
        Sandwich ELISA - Anti-ALIX antibody [EPR23653-13] - BSA and Azide free (Capture) (ab277682)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (0)

      Publishing research using ab277682? Please let us know so that we can cite the reference in this datasheet.

      ab277682 has not yet been referenced specifically in any publications.

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