Alkaline Phosphatase Anti-Human Serum Albumin antibody (ab19181)
Key features and details
- Alkaline Phosphatase Goat polyclonal to Human Serum Albumin
- Suitable for: ELISA, ICC, WB
- Reacts with: Human
- Conjugation: Alkaline Phosphatase
- Isotype: IgG
Overview
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Product name
Alkaline Phosphatase Anti-Human Serum Albumin antibody
See all Human Serum Albumin primary antibodies -
Description
Alkaline Phosphatase Goat polyclonal to Human Serum Albumin -
Host species
Goat -
Conjugation
Alkaline Phosphatase -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with human albumin. -
Tested applications
Suitable for: ELISA, ICC, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Human Human Serum Albumin. Serum albumin
Database link: P02768 -
General notes
Molar enzyme:antibody protein ratio is 1:1.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.1% Sodium azide
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to alkaline phosphatase (alkphos). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab19181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| ICC |
Use at an assay dependent concentration.
|
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| WB |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
ICC
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. |
Target
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Function
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Post-translational
modificationsKenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 213 Human
- Omim: 103600 Human
- SwissProt: P02768 Human
- Unigene: 418167 Human
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Alternative names
- alb antibody
- ALBU_HUMAN antibody
- Albumin (32 AA) antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab19181 has been referenced in 1 publication.
- Fujimi TJ et al. Investigation of the effects of urea cycle amino acids on the expression of ALB and CEBPB in the human hepatocellular carcinoma cell line FLC-4. Hum Cell 33:590-598 (2020). PubMed: 32474770