Anti-ALP antibody (ab154100)
Key features and details
- Rabbit polyclonal to ALP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ALP antibody
See all ALP primary antibodies -
Description
Rabbit polyclonal to ALP -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 89-266 of Human ALP.
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Positive control
- 293T, A431, HepG2 whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 39 kDa.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 39 kDa. |
Target
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Function
May play a role in the organization of actin filament arrays within muscle cells. -
Tissue specificity
Isoform 1 is highly expressed in differentiated skeletal muscle. Isoform 2 is heart-specific. -
Sequence similarities
Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain. -
Cellular localization
Cytoplasm > myofibril > sarcomere > Z line. Localizes to myofiber Z-lines. - Information by UniProt
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Database links
- Entrez Gene: 538516 Cow
- Entrez Gene: 27295 Human
- Entrez Gene: 53318 Mouse
- Omim: 605889 Human
- SwissProt: Q3SYZ8 Cow
- SwissProt: Q53GG5 Human
- SwissProt: O70209 Mouse
- Unigene: 442702 Human
see all -
Alternative names
- Actinin associated LIM protein antibody
- Actinin-associated LIM protein antibody
- Alpha actinin 2 associated LIM protein antibody
see all
Images
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Western blot - Anti-ALP antibody (ab154100)All lanes : Anti-ALP antibody (ab154100) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
10% SDS PAGE
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab154100 has been referenced in 3 publications.
- Chang Z et al. Construction and evaluation of a novel tissue-engineered bone device. Exp Ther Med 22:1166 (2021). PubMed: 34504611
- She K et al. FAIM2 Promotes Non-Small Cell Lung Cancer Cell Growth and Bone Metastasis by Activating the Wnt/β-Catenin Pathway. Front Oncol 11:690142 (2021). PubMed: 34568020
- Jiang Z & Hua Y Hydrogen sulfide promotes osteogenic differentiation of human periodontal ligament cells via p38-MAPK signaling pathway under proper tension stimulation. Arch Oral Biol 72:8-13 (2016). PubMed: 27522508