Anti-ALP antibody (ab67228)
Key features and details
- Mouse polyclonal to ALP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ALP antibody
See all ALP primary antibodies -
Description
Mouse polyclonal to ALP -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human ALP. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- ALP transfected 293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab67228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 39 kDa).
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| Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 39 kDa). |
Target
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Function
May play a role in the organization of actin filament arrays within muscle cells. -
Tissue specificity
Isoform 1 is highly expressed in differentiated skeletal muscle. Isoform 2 is heart-specific. -
Sequence similarities
Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain. -
Cellular localization
Cytoplasm > myofibril > sarcomere > Z line. Localizes to myofiber Z-lines. - Information by UniProt
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Database links
- Entrez Gene: 27295 Human
- Omim: 605889 Human
- SwissProt: Q53GG5 Human
- Unigene: 442702 Human
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Alternative names
- Actinin associated LIM protein antibody
- Actinin-associated LIM protein antibody
- Alpha actinin 2 associated LIM protein antibody
see all
Images
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Western blot - Anti-ALP antibody (ab67228)All lanes : Anti-ALP antibody (ab67228) at 1/500 dilution
Lane 1 : ALP transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 39 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (19)
ab67228 has been referenced in 19 publications.
- Sun B et al. Irisin reduces bone fracture by facilitating osteogenesis and antagonizing TGF-β/Smad signaling in a growing mouse model of osteogenesis imperfecta. J Orthop Translat 38:175-189 (2023). PubMed: 36439629
- Li Y et al. LincRNA-EPS increases TGF-β expression to inhibit the Wnt/β-catenin pathway, VSMC osteoblastic differentiation and vascular calcification in diabetic mice. Exp Ther Med 23:425 (2022). PubMed: 35607373
- Li H et al. Indoleamine 2,3-dioxygenase mediates the therapeutic effects of adipose-derived stromal/stem cells in experimental periodontitis by modulating macrophages through the kynurenine-AhR-NRF2 pathway. Mol Metab 66:101617 (2022). PubMed: 36270612
- Nirwana I et al. Ellagic acid and hydroxyapatite promote angiogenesis marker in bone defect. J Oral Biol Craniofac Res 12:116-120 (2022). PubMed: 34840942
- Li D et al. The miR-4739/DLX3 Axis Modulates Bone Marrow-Derived Mesenchymal Stem Cell (BMSC) Osteogenesis Affecting Osteoporosis Progression. Front Endocrinol (Lausanne) 12:703167 (2021). PubMed: 34925225
