Recombinant Anti-ALP antibody [EPR27191-14] (ab307726)
![Western blot - Anti-ALP antibody [EPR27191-14] (ab307726)](https://www.abcam.com/ps/products/307/ab307726/Images/ab307726-500853-anti-alp-antibody-epr27191-14-western-blot-293t-cells-transfected-with-an-empty-vector-containing-a-his-tag-whole-cell-lysate-20-ug.jpg "Western blot - Anti-ALP antibody [EPR27191-14] (ab307726)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR27191-14] to ALP
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ALP antibody [EPR27191-14]
See all ALP primary antibodies -
Description
Rabbit monoclonal [EPR27191-14] to ALP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF,IHC-Fr or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human skeletal muscle and 293T cells transfected with a human PDLIM3 expression vector containing a His-tag. IHC-P: Human skeletal muscle.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR27191-14 -
Research areas
Associated products
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Alternative Versions
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab307726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 39 kDa.
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| IHC-P |
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000. Predicted molecular weight: 39 kDa. |
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IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May play a role in the organization of actin filament arrays within muscle cells. -
Tissue specificity
Isoform 1 is highly expressed in differentiated skeletal muscle. Isoform 2 is heart-specific. -
Sequence similarities
Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain. -
Cellular localization
Cytoplasm > myofibril > sarcomere > Z line. Localizes to myofiber Z-lines. - Information by UniProt
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Database links
- Entrez Gene: 27295 Human
- Omim: 605889 Human
- SwissProt: Q53GG5 Human
- Unigene: 442702 Human
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Alternative names
- Actinin associated LIM protein antibody
- Actinin-associated LIM protein antibody
- Alpha actinin 2 associated LIM protein antibody
see all
Images
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Western blot - Anti-ALP antibody [EPR27191-14] (ab307726)All lanes : Anti-ALP antibody [EPR27191-14] (ab307726) at 1/1000 dilution
Lane 1 : 293T cells transfected with an empty vector containing a his tag whole cell lysate 20 µg
Lane 2 : 293T cells transfected with a human PDLIM3 expression vector containing a His-tag whole cell lysate 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 39 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The bands beneath the target band (43 kDa) are likely to be degraded target fragments.
In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution.
Exposure time: 10 seconds
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![Western blot - Anti-ALP antibody [EPR27191-14] (ab307726)](https://www.abcam.com/ps/products/307/ab307726/Images/ab307726-500852-anti-alp-antibody-epr27191-14-western-blot-human-skeletal-muscle-tissue-lysate-20-ug.jpg)
Western blot - Anti-ALP antibody [EPR27191-14] (ab307726)All lanes : Anti-ALP antibody [EPR27191-14] (ab307726) at 1/1000 dilution
Lane 1 : Human skeletal muscle tissue lysate 20 µg
Lane 2 : Human kidney tissue lysate 20 µg
Secondary
Lane 1 : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Lane 2 : Goat Anti-Rbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 39 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: human kidney (PMID: 9334352).
The bands beneath the target band (43 kDa) are likely to be degraded target fragments.
In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.
Exposure time: 180 seconds
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALP antibody [EPR27191-14] (ab307726)](https://www.abcam.com/ps/products/307/ab307726/Images/ab307726-3-anti-alp-antibody-epr27191-14-immunohistochemistry-human-kidney.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALP antibody [EPR27191-14] (ab307726)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ALP with ab307726 at 1/1000 (0.254 µg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.
Negative control: no staining on the human kidney (PMID:9334352). The section was incubated with ab307726 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.
Heat mediated antigen retrieval was performed with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALP antibody [EPR27191-14] (ab307726)](https://www.abcam.com/ps/products/307/ab307726/Images/ab307726-2-anti-alp-antibody-epr27191-14-immunohistochemistry-human-skeletal-muscl.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALP antibody [EPR27191-14] (ab307726)Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling ALP with ab307726 at 1/1000 (0.254 µg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Postive staining on human skeletal muscle (PMID:9334352). The section was incubated with ab307726 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.
Heat mediated antigen retrieval was performed with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
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![Anti-ALP antibody [EPR27191-14] (ab307726)](https://www.abcam.com/ps/products/307/ab307726/Images/ab307726-1-benefitsofrecombinantantibodies.jpg)
Anti-ALP antibody [EPR27191-14] (ab307726)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab307726 has not yet been referenced specifically in any publications.