Recombinant Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)
![Western blot - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)](https://www.abcam.com/ps/products/133/ab133617/Images/ab133617-449557-anti-alpha-1-fetoprotein-antibody-epafp61-westernblot-hepg2-human.jpg "Western blot - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPAFP61] to alpha 1 Fetoprotein
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-alpha 1 Fetoprotein antibody [EPAFP61]
See all alpha 1 Fetoprotein primary antibodies -
Description
Rabbit monoclonal [EPAFP61] to alpha 1 Fetoprotein -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human alpha 1 Fetoprotein.
Database link: P02771 -
Positive control
- Flow Cyt (intra): HepG2 cells; IP: HepG2 whole cell lysate; ICC/IF: HepG2 cells; IHC-P: Human liver cancer tissue; WB: HepG2 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPAFP61 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 488 Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab199231)
- Alexa Fluor® 647 Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab199469)
- Anti-alpha 1 Fetoprotein antibody [EPAFP61] - Low endotoxin, Azide free (ab215379)
- Anti-alpha 1 Fetoprotein antibody [EPAFP61] - BSA and Azide free (ab271896)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133617 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
|
|
| ICC/IF |
1/250.
|
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. Heat up to 98°C, below boiling, and then let cool for 10-20 min. |
|
| IP |
1/10 - 1/100.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa). |
|
ICC/IF
1/250. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. Heat up to 98°C, below boiling, and then let cool for 10-20 min. |
|
IP
1/10 - 1/100. |
Target
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Function
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. -
Tissue specificity
Plasma. Synthesized by the fetal liver and yolk sac. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Developmental stage
Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma. -
Post-translational
modificationsIndependent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 174 Human
- Omim: 104150 Human
- SwissProt: P02771 Human
- Unigene: 518808 Human
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Alternative names
- Afp antibody
- AFPD antibody
- Alpha fetoglobulin antibody
see all
Images
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Western blot - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617) at 1/10000 dilution (Purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 68 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)](https://www.abcam.com/ps/products/133/ab133617/Images/ab133617-16-anti-alpha-1-fetoprotein-antibody-epafp61-immunocytochemistry-hepg2-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)Immunocytochemistry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling alpha 1 Fetoprotein with Purified ab133617 at 1/250 dilution (0.52 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)](https://www.abcam.com/ps/products/133/ab133617/Images/ab133617-15-anti-alpha-1-fetoprotein-antibody-epafp61-immunohistochemistry-liver-cancer-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver cancer tissue sections labeling alpha 1 Fetoprotein with Purified ab133617 at 1/100 dilution (1.3 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)](https://www.abcam.com/ps/products/133/ab133617/Images/ab133617-14-anti-alpha-1-fetoprotein-antibody-epafp61-immunoprecipitation-hepg2-human.jpg)
Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)Purified ab133617 at 1/20 dilution (0.5µg) immunoprecipitating alpha 1 Fetoprotein in HepG2 whole cell lysate.
Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab133617 + HepG2 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133617 in HepG2 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/5000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 70 kDa
Lower bands could be caspase-dependent cleavage fragments. (PMID: 31811908) -
![Flow Cytometry (Intracellular) - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)](https://www.abcam.com/ps/products/133/ab133617/Images/ab133617-13-anti-alpha-1-fetoprotein-antibody-epafp61-flowcytometry-hepg2-human.jpg)
Flow Cytometry (Intracellular) - Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)Intracellular Flow Cytometry analysis of HepG2 Human hepatocellular carcinoma epithelial cell cells labeling alpha 1 Fetoprotein with purified ab133617 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
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![Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)](https://www.abcam.com/ps/products/133/ab133617/Images/ab133617-11-benefits-of-recombinant-antibodies.png)
Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab133617 has been referenced in 4 publications.
- van Schaijik B et al. Proliferating infantile hemangioma promotes a-fetoprotein production by HepG2 cells. Pediatr Res 87:3-6 (2020). PubMed: 31412353
- Castro-Viñuelas R et al. Generation and characterization of human induced pluripotent stem cells (iPSCs) from hand osteoarthritis patient-derived fibroblasts. Sci Rep 10:4272 (2020). PubMed: 32144293
- Russell OM et al. Preferential amplification of a human mitochondrial DNA deletion in vitro and in vivo. Sci Rep 8:1799 (2018). PubMed: 29379065
- Yang YH et al. Generation of Induced Pluripotent Stem Cells from Human Epidermal Keratinocytes. Cell Reprogram 20:356-364 (2018). PubMed: 30388030