Recombinant Anti-alpha 1 Fetoprotein antibody [EPR19240-133] (ab199054)
![Western blot - Anti-alpha 1 Fetoprotein antibody [EPR19240-133] (ab199054)](https://www.abcam.com/ps/products/199/ab199054/Images/ab199054-287582-anti-alpha-1-fetoprotein-antibody-epr19240-133-western-blot.jpg "Western blot - Anti-alpha 1 Fetoprotein antibody [EPR19240-133] (ab199054)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19240-133] to alpha 1 Fetoprotein
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse
Related conjugates and formulations
Overview
-
Product name
Anti-alpha 1 Fetoprotein antibody [EPR19240-133]
See all alpha 1 Fetoprotein primary antibodies -
Description
Rabbit monoclonal [EPR19240-133] to alpha 1 Fetoprotein -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Mouse placenta and fetal liver lysates; Hepa1-6 whole cell lysate. ICC/IF: Hepa1-6 cells. IP: Mouse placenta lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19240-133 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab199054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 67 kDa).
|
|
| ICC/IF |
1/100.
|
|
| IP |
1/30.
|
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 67 kDa). |
|
ICC/IF
1/100. |
|
IP
1/30. |
Target
-
Function
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. -
Tissue specificity
Plasma. Synthesized by the fetal liver and yolk sac. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Developmental stage
Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma. -
Post-translational
modificationsIndependent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 11576 Mouse
- SwissProt: P02772 Mouse
- Unigene: 358570 Mouse
-
Alternative names
- Afp antibody
- AFPD antibody
- Alpha fetoglobulin antibody
see all
Images
-
Western blot - Anti-alpha 1 Fetoprotein antibody [EPR19240-133] (ab199054)All lanes : Anti-alpha 1 Fetoprotein antibody [EPR19240-133] (ab199054) at 1/1000 dilution
Lane 1 : Mouse placenta lysate
Lane 2 : Mouse fetal liver lysate
Lane 3 : Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 67 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Exposure time : Lanes 1 and 2: 3 seconds; Lane 3: 3 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
The observed molecular weight is consistent with the literature (PMID: 18525461).
-
![Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPR19240-133] (ab199054)](https://www.abcam.com/ps/products/199/ab199054/Images/ab199054-287581-anti-alpha-1-fetoprotein-antibody-epr19240-133-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPR19240-133] (ab199054)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cells labeling alpha 1 Fetoprotein with ab199054 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Hepa1-6 cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
-
![Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPR19240-133] (ab199054)](https://www.abcam.com/ps/products/199/ab199054/Images/ab199054-287579-anti-alpha-1-fetoprotein-antibody-epr19240-133-immunoprecipitation.jpg)
Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPR19240-133] (ab199054)alpha 1 Fetoprotein was immunoprecipitated from 0.35 mg of mouse placenta lysate with ab199054 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199054 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse placenta lysate 10 µg (Input).
Lane 2: ab199054 IP in mouse placenta lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199054 in mouse placenta lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
-
![Anti-alpha 1 Fetoprotein antibody [EPR19240-133] (ab199054)](https://www.abcam.com/ps/products/199/ab199054/Images/ab199054-4-benefits-of-recombinant-antibodies.png)
Anti-alpha 1 Fetoprotein antibody [EPR19240-133] (ab199054)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (1)
ab199054 has been referenced in 1 publication.
- Chen Z et al. Transforming growth factor-β signaling confers hepatic stellate cells progenitor features after partial hepatectomy. J Cell Physiol 235:2655-2667 (2020). PubMed: 31584200