Recombinant Anti-alpha 1 Fetoprotein antibody [EPR20667] (ab213328)
![Western blot - Anti-alpha 1 Fetoprotein antibody [EPR20667] (ab213328)](https://www.abcam.com/ps/products/213/ab213328/Images/ab213328-280319-anti-alpha-1-fetoprotein-antibody-epr20667-western-blot.jpg "Western blot - Anti-alpha 1 Fetoprotein antibody [EPR20667] (ab213328)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20667] to alpha 1 Fetoprotein
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
-
Product name
Anti-alpha 1 Fetoprotein antibody [EPR20667]
See all alpha 1 Fetoprotein primary antibodies -
Description
Rabbit monoclonal [EPR20667] to alpha 1 Fetoprotein -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Hepa 1-6 whole cell lysate; Mouse fetal liver and placenta lysates; Rat placenta lysate. ICC/IF: Hepa 1-6 cells. Flow Cyt (intra): Hepa 1-6 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20667 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab213328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
|
|
| WB |
1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 67 kDa).
|
|
| ICC/IF | (1) |
1/100.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/500. |
|
WB
1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 67 kDa). |
|
ICC/IF
1/100. |
Target
-
Function
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. -
Tissue specificity
Plasma. Synthesized by the fetal liver and yolk sac. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Developmental stage
Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma. -
Post-translational
modificationsIndependent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 11576 Mouse
- Entrez Gene: 24177 Rat
- SwissProt: P02772 Mouse
- SwissProt: P02773 Rat
- Unigene: 358570 Mouse
- Unigene: 9174 Rat
-
Alternative names
- Afp antibody
- AFPD antibody
- Alpha fetoglobulin antibody
see all
Images
-
Western blot - Anti-alpha 1 Fetoprotein antibody [EPR20667] (ab213328)Lanes 1-2 : Anti-alpha 1 Fetoprotein antibody [EPR20667] (ab213328) at 1/1000 dilution
Lanes 3-4 : Anti-alpha 1 Fetoprotein antibody [EPR20667] (ab213328) at 1/5000 dilution
Lane 1 : Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate
Lane 2 : Rat placenta lysate
Lane 3 : Mouse fetal liver lysate
Lane 4 : Mouse placenta lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 67 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 10 seconds; Lane 2/4: 1 second; Lane 3: 3 seconds.
The observed molecular weight is consistent with the literature (PMID: 18525461).
-
![Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPR20667] (ab213328)](https://www.abcam.com/ps/products/213/ab213328/Images/ab213328-280318-anti-alpha-1-fetoprotein-antibody-epr20667-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPR20667] (ab213328)Immunofluorescent analysis of 4% paraformaldehyde-fixed. 0.1% Triton X-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cells labeling alpha 1 Fetoprotein with ab213328 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Hepa1-6 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
-
![Flow Cytometry (Intracellular) - Anti-alpha 1 Fetoprotein antibody [EPR20667] (ab213328)](https://www.abcam.com/ps/products/213/ab213328/Images/ab213328-280317-anti-alpha-1-fetoprotein-antibody-epr20667-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-alpha 1 Fetoprotein antibody [EPR20667] (ab213328)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Hepa1-6 (mouse hepatoma epithelial cell line) cell line labeling alpha 1 Fetoprotein with ab213328 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
-
![Anti-alpha 1 Fetoprotein antibody [EPR20667] (ab213328)](https://www.abcam.com/ps/products/213/ab213328/Images/ab213328-4-benefits-of-recombinant-antibodies.png)
Anti-alpha 1 Fetoprotein antibody [EPR20667] (ab213328)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (3)
ab213328 has been referenced in 3 publications.
- Lee J et al. In vitro generation of functional murine heart organoids via FGF4 and extracellular matrix. Nat Commun 11:4283 (2020). PubMed: 32883967
- Ma XH et al. Development and in vitro study of a bi-specific magnetic resonance imaging molecular probe for hepatocellular carcinoma. World J Gastroenterol 25:3030-3043 (2019). PubMed: 31293339
- Wang D et al. Hepatectomy promotes recurrence of liver cancer by enhancing IL-11-STAT3 signaling. EBioMedicine 46:119-132 (2019). PubMed: 31375423