Recombinant Anti-alpha 1 Fetoprotein antibody [EPR22560-268] (ab255813)
![Western blot - Anti-alpha 1 Fetoprotein antibody [EPR22560-268] (ab255813)](https://www.abcam.com/ps/products/255/ab255813/Images/ab255813-3-anti-alpha-1-fetoprotein-antibody-epr-22560-268-western-blot-rat.jpg "Western blot - Anti-alpha 1 Fetoprotein antibody [EPR22560-268] (ab255813)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22560-268] to alpha 1 Fetoprotein
- Suitable for: WB, IP
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-alpha 1 Fetoprotein antibody [EPR22560-268]
See all alpha 1 Fetoprotein primary antibodies -
Description
Rabbit monoclonal [EPR22560-268] to alpha 1 Fetoprotein -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: ICC/IF,IHC-Fr or IHC-P -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Hepa1-6 whole cell lysate. Mouse and rat placenta tissue lysate. Rat liver tissue lysate. IP: Rat placenta lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22560-268 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab255813 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/30. Predicted molecular weight: 68 kDa.
|
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| IP |
1/30.
|
| Notes |
|---|
|
WB
1/1000 - 1/30. Predicted molecular weight: 68 kDa. |
|
IP
1/30. |
Target
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Function
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. -
Tissue specificity
Plasma. Synthesized by the fetal liver and yolk sac. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Developmental stage
Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma. -
Post-translational
modificationsIndependent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 11576 Mouse
- Entrez Gene: 24177 Rat
- SwissProt: P02772 Mouse
- SwissProt: P02773 Rat
- Unigene: 358570 Mouse
- Unigene: 9174 Rat
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Alternative names
- Afp antibody
- AFPD antibody
- Alpha fetoglobulin antibody
see all
Images
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Western blot - Anti-alpha 1 Fetoprotein antibody [EPR22560-268] (ab255813)All lanes : Anti-alpha 1 Fetoprotein antibody [EPR22560-268] (ab255813) at 1/1000 dilution
Lane 1 : Rat placenta tissue lysate
Lane 2 : Rat liver tissue lysate
Lane 3 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 68 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 25355916).
Negative control: Rat brain (PMID: 2410773, 6175646 ).
Exposure times: Lane 1: 1 sec; Lanes 2-3: 3 mins.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-alpha 1 Fetoprotein antibody [EPR22560-268] (ab255813)](https://www.abcam.com/ps/products/255/ab255813/Images/ab255813-2-anti-alpha-1-fetoprotein-antibody-epr-22560-268-western-blot-mouse.jpg)
Western blot - Anti-alpha 1 Fetoprotein antibody [EPR22560-268] (ab255813)All lanes : Anti-alpha 1 Fetoprotein antibody [EPR22560-268] (ab255813) at 1/1000 dilution
Lane 1 : Hepa1-6 (mouse hepatoma epithelial cell), whole cell lysate
Lane 2 : Mouse placenta tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 68 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 25355916).
Exposure times: Lane 1: 3 mins; Lane 2: 5.5 secs.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPR22560-268] (ab255813)](https://www.abcam.com/ps/products/255/ab255813/Images/ab255813-1-anti-alpha-1-fetoprotein-antibody-epr-22560-268-immunoprecipitation-placenta-rat.jpg)
Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPR22560-268] (ab255813)alpha 1 Fetoprotein was immunoprecipitated from 0.35 mg rat placenta lysate with ab255813 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab255813 at 1/1000 dilution (0.56 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at a 1/5000 dilution.
Lane 1: Rat placenta lysate 10μg
Lane 2: ab255813 IP in rat placenta lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab255813 in rat placenta lysate.Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 secs. -
![Anti-alpha 1 Fetoprotein antibody [EPR22560-268] (ab255813)](https://www.abcam.com/ps/products/255/ab255813/Images/ab255813-4-benefits-of-recombinant-antibodies.png)
Anti-alpha 1 Fetoprotein antibody [EPR22560-268] (ab255813)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab255813 has not yet been referenced specifically in any publications.