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    products/primary-antibodies/alpha-defensin-1--2--3-antibody-ab99504.pdf

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Microbiology Protein Human Protein Defensin
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Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504)

  • Datasheet
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Western blot - Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504)

    Key features and details

    • Goat polyclonal to alpha Defensin 1 + 2 + 3
    • Suitable for: IHC-P, WB
    • Reacts with: Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-alpha Defensin 1 + 2 + 3 antibody
    • Description

      Goat polyclonal to alpha Defensin 1 + 2 + 3
    • Host species

      Goat
    • Specificity

      ab99504 is expected to equally recognize alpha Defensin 1 precursor and alpha Defensin 3 precursor.
    • Tested applications

      Suitable for: IHC-P, WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide:

      C-DESLAPKHPGSRKN

      , corresponding to internal sequence amino acids 50-63 of Human alpha Defensin 1 + 2 + 3 (NP_004075.1; NP_005208.1).
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • WB: Human spleen lysate
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 0.5% BSA, Tris buffered saline
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Microbiology
      • Protein
      • Human Protein
      • Defensin
      • Immunology
      • Secreted Molecules
      • Other secreted molecules

    Associated products

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab99504 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    WB
    Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 10 kDa.

    1 hour primary incubation is recommended for this product.

    Notes
    IHC-P
    Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    WB
    Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 10 kDa.

    1 hour primary incubation is recommended for this product.

    Target

    • Relevance

      Defensins are a group of cyclic peptides containing 29-35 amino acids, which comprise approximately 30% of the total protein content of neutrophil azurophilic granules. Various functions have been reported for the defensins, including antibacterial and antifungal activity and chemotactic activity for monocytes.
    • Cellular localization

      Secreted
    • Database links

      • Entrez Gene: 1667 Human
      • Omim: 125220 Human
      • SwissProt: P59665 Human
      • Alternative names

        • DEF1 antibody
        • DEF3 antibody
        • DEFA1 antibody
        • DEFA2 antibody
        • DEFA3 antibody
        • Defensin alpha 1 antibody
        • Defensin alpha 2 antibody
        • Defensin alpha 3 antibody
        • HNP1 antibody
        • HNP3 antibody
        • HP1 antibody
        • HP3 antibody
        • MRS antibody
        • Neutrophil defensin 1 antibody
        • Neutrophil defensin 2 antibody
        • Neutrophil defensin 3 antibody
        see all

      Images

      • Western blot - Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504)
        Western blot - Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504)
        Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504) at 2 µg/ml + Human Spleen lysate in RIPA buffer at 35 µg

        Developed using the ECL technique.

        Predicted band size: 10 kDa


        Exposure time: 1 hour

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (0)

      Publishing research using ab99504? Please let us know so that we can cite the reference in this datasheet.

      ab99504 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Question

      Je recherche un anticorps anti-defensin/cryptdin pour une utilisation en IHC-P sur du tissu de souris.

      J'ai repéré le Anti-alpha Defensin 1 + 2 + 3 antibody (ab99504) ou Anti-alpha Defensin NP5 antibody [8C8] (ab62757) qui reconnaissent l'humain.

      Avez vous des infos sur sa compatibilité avec le tissu murin?

      Read More

      Abcam community

      Verified customer

      Asked on Mar 26 2013

      Answer



      Nous n’avons jamais testé ni ab99504 ni ab62757 chez la souris en IHC-P.

      J’ai fait une comparaison entre l’alpha Defensin 1 + 2 + 3 humaine et murin et il n’y qu’une homologie totale d’environ 30%. J’ai aussi fait une comparaison entre la protéine humaine et murin alpha Defensin NP5 et j’ai trouvé que la protéine totale avait 33% d’homologie. Cela suggère que malheureusement aucun anticorps anti-humain alpha Defensin fonctionnera sur des tissue murins.

      Read More

      Abcam Scientific Support

      Answered on Mar 26 2013

      Question

      searching for an anti NP 1 alpha defensin ELISA kit to test on human samples

      Read More

      Abcam community

      Verified customer

      Asked on Sep 07 2011

      Answer

      Vielen Dank für Ihren Anruf und Ihr Interesse an unseren Produkten. Leider haben wir zur Zeit kein ELISA Kit in unserem Katalog gegen das NP-1/alpha defensin 1 protein. Wir haben drei Antikörper, die jedoch auch andere zyklische alpha Defensin erkennen können. Wir haben zur Zeit auch keinen Antikörper der nur das NP1 erkennen würde. ab90486: Dieser monokonale Antikörper aus der Maus erkennt das humane NP1, NP2 und NP3. Dieser Antikörper wurde auch in ELISA und Western blot getestet und ist darum auch von uns in diesen Applikationen garantiert. Click here (or use the following: https://www.abcam.com/alpha-Defensin-1-antibody-B539M-ab90486.html). ab99504: Dieser polyklonale Antikörper aus der Ziege wurde nur in Western blot getestet bislang, und erkennt auch die humanen NP1, NP2 und NP3. Falls Sie diesen Antikörper in ELISA testen möchten, könnten wir Ihnen ein Testangebot machen. Bitte lassen Sie mich wissen ob Sie dies Interessiert. Click here (or use the following: https://www.abcam.com/alpha-Defensin-1-2-3-antibody-ab99504.html). ab64763: Dieser monoklonale Antikörper aus der Maus erkennt auch NP1, NP2 und NP3 aus dem Menschen und wurde auch in ELISA und Western blot getestet. Click here (or use the following: https://www.abcam.com/alpha-Defensin-1-2-3-antibody-D1F4-ab64763.html). Bitte lassen Sie mich wissen falls Sie weitere Fragen haben oder ob das Testangebot Sie interessieren könnte. Ich wünsche Ihnen einen ganz schönen Nachmittag noch.

      Read More

      Abcam Scientific Support

      Answered on Sep 07 2011

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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