Recombinant Anti-alpha Sarcoglycan antibody [IVD3-A9] - BSA and Azide free (ab255739)
![Immunohistochemistry (Frozen sections) - Anti-alpha Sarcoglycan antibody [IVD3-A9] - BSA and Azide free (ab255739)](https://www.abcam.com/ps/products/255/ab255739/Images/ab255739-481754-ab2345891antialphasarcoglycanantibodyivd3a9immunohistochemistryfrozenmouseheartfresh.jpg "Immunohistochemistry (Frozen sections) - Anti-alpha Sarcoglycan antibody [IVD3-A9] - BSA and Azide free (ab255739)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [IVD3-A9] to alpha Sarcoglycan - BSA and Azide free
- Suitable for: IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-alpha Sarcoglycan antibody [IVD3-A9] - BSA and Azide free
See all alpha Sarcoglycan primary antibodies -
Description
Mouse monoclonal [IVD3-A9] to alpha Sarcoglycan - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IHC-Frmore details
Unsuitable for: ICC,IHC-P or WB -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-Fr: Human, mouse and rat heart tissue.
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General notes
ab255739 is the carrier free version of ab234589.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
IVD3-A9 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab255739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
Target
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Function
Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix. -
Tissue specificity
Most strongly expressed in skeletal muscle. Also expressed in cardiac muscle and, at much lower levels, in lung. In the fetus, most abundant in cardiac muscle and, at lower levels, in lung. Also detected in liver and kidney. Not expressed in brain. -
Involvement in disease
Defects in SGCA are the cause of limb-girdle muscular dystrophy type 2D (LGMD2D) [MIM:608099]; also known as Duchenne-like muscular dystrophy autosomal recessive type 2 or severe childhood autosomal recessive muscular dystrophy (SCARMD). LGMD2D is an autosomal recessive degenerative myopathy characterized by progressive muscle wasting from early childhood with loss of independent ambulation by teenage years. Muscle biopsy shows necrosis, decreased immunostaining for alpha sarcoglycan, and adhalin deficiency. The phenotype is less severe than LGMD2C. -
Sequence similarities
Belongs to the sarcoglycan alpha/epsilon family. -
Cellular localization
Cell membrane > sarcolemma. Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 6442 Human
- Entrez Gene: 20391 Mouse
- Entrez Gene: 303468 Rat
- Omim: 600119 Human
- SwissProt: Q16586 Human
- SwissProt: P82350 Mouse
- SwissProt: Q5SWB2 Mouse
- Unigene: 463412 Human
see all -
Alternative names
- 50 DAG antibody
- 50 kDa dystrophin associated glycoprotein antibody
- 50 kDa dystrophin-associated glycoprotein antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-alpha Sarcoglycan antibody [IVD3-A9] - BSA and Azide free (ab255739)
This data was developed using ab234589, the same clone but in a different formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse heart (fresh) tissue labeling alpha Sarcoglycan with ab234589 at a 1/100 dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/ml) (Green). Membranous staining on mouse heart is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at a 1/1000 dilution.
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![Immunohistochemistry (Frozen sections) - Anti-alpha Sarcoglycan antibody [IVD3-A9] - BSA and Azide free (ab255739)](https://www.abcam.com/ps/products/255/ab255739/Images/ab255739-481756-antialphasarcoglycanantibodyivd3a9immunohistochemistryfrozenhumanheart.jpg)
Immunohistochemistry (Frozen sections) - Anti-alpha Sarcoglycan antibody [IVD3-A9] - BSA and Azide free (ab255739)This data was developed using ab234589, the same clone but in a different formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen human heart tissue labeling alpha Sarcoglycan. Membranous staining on human heart. The section was blocked using Bloxall™ for 20 mins at room temperature, then incubated with ab234589, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. It was then counterstained with haematoxylin and mounted with DPX. The nuclear counterstain was DAPI (Blue).
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![Immunohistochemistry (Frozen sections) - Anti-alpha Sarcoglycan antibody [IVD3-A9] - BSA and Azide free (ab255739)](https://www.abcam.com/ps/products/255/ab255739/Images/ab255739-481755-ab2345892antialphasarcoglycanantibodyivd3a9immunohistochemistryfrozenmousespleenfresh.jpg)
Immunohistochemistry (Frozen sections) - Anti-alpha Sarcoglycan antibody [IVD3-A9] - BSA and Azide free (ab255739)This data was developed using ab234589, the same clone but in a different formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse spleen (fresh) tissue labeling alpha Sarcoglycan with ab234589 at a 1/100 dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at a 1/1000 dilution (Green). Negative control: No staining on mouse spleen is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at a 1/1000 dilution.
-
![Immunohistochemistry (Frozen sections) - Anti-alpha Sarcoglycan antibody [IVD3-A9] - BSA and Azide free (ab255739)](https://www.abcam.com/ps/products/255/ab255739/Images/ab255739-481757-antialphasarcoglycanantibodyivd3a9immunohistochemistryfrozenratheart.jpg)
Immunohistochemistry (Frozen sections) - Anti-alpha Sarcoglycan antibody [IVD3-A9] - BSA and Azide free (ab255739)This data was developed using ab234589, the same clone but in a different formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat heart tissue labeling alpha Sarcoglycan. Membranous staining on human heart. The section was blocked using Bloxall™ for 20 mins at room temperature, then incubated with ab234589, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. It was then counterstained with haematoxylin and mounted with DPX. The nuclear counterstain was DAPI (Blue).
-
![Anti-alpha Sarcoglycan antibody [IVD3-A9] - BSA and Azide free (ab255739)](https://www.abcam.com/ps/products/255/ab255739/Images/ab255739-481758-BenefitsofRecombinantantibodies.jpg)
Anti-alpha Sarcoglycan antibody [IVD3-A9] - BSA and Azide free (ab255739)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab255739 has not yet been referenced specifically in any publications.
