Recombinant Anti-alpha smooth muscle Actin antibody [BLR082G] (ab265588)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR082G] to alpha smooth muscle Actin
- Suitable for: WB, IHC-P, ICC
- Reacts with: Mouse, Human
Overview
-
Product name
Anti-alpha smooth muscle Actin antibody [BLR082G]
See all alpha smooth muscle Actin primary antibodies -
Description
Rabbit monoclonal [BLR082G] to alpha smooth muscle Actin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICCmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human alpha smooth muscle Actin aa 1-50. The exact sequence is proprietary. NP_001135417.1
Database link: P62736 -
Positive control
- IHC-P: Human breast tissue. Human colon carcinoma tissue. ICC: IMR-90 cells. WB: IMR-90 and NIH/3T3 whole cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
BLR082G -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab265588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 42 kDa.
|
|
| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| ICC |
1/100 - 1/500.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 42 kDa. |
|
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. -
Sequence similarities
Belongs to the actin family. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
-
Database links
- Entrez Gene: 59 Human
- Entrez Gene: 11475 Mouse
- Omim: 102620 Human
- SwissProt: P62736 Human
- SwissProt: P62737 Mouse
- Unigene: 500483 Human
- Unigene: 213025 Mouse
-
Alternative names
- a actin antibody
- AAT6 antibody
- ACTA_HUMAN antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab265588)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling alpha smooth muscle Actin with ab265588 at 1/100 dilution. Detection: DAB.
-
Western blot - Anti-alpha smooth muscle Actin antibody (ab265588)All lanes : Anti-alpha smooth muscle Actin antibody [BLR082G] (ab265588) at 1/1000 dilution
Lane 1 : MCF7 whole cell lysate at 50 µg
Lane 2 : A549 whole cell lysate at 50 µg
Lane 3 : Jurkat whole cell lysate at 50 µg
Lane 4 : IMR-90 whole cell lysate at 10 µg
Lane 5 : HepG2 whole cell lysate at 50 µg
Lane 6 : LNCaP whole cell lysate at 50 µg
Lane 7 : RKO whole cell lysate at 50 µg
Lane 8 : K562 whole cell lysate at 50 µg
Lane 9 : NIH/3T3 whole cell lysate at 50 µg
Lane 10 : TCMK whole cell lysate at 50 µg
Predicted band size: 42 kDa
Exposure time: 30 secondsCells prepared using NETN lysis buffer.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab265588)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast tissue labeling alpha smooth muscle Actin with ab265588 at 1/100 dilution. Detection: DAB.
-
Immunocytochemistry - Anti-alpha smooth muscle Actin antibody (ab265588)Immunocytochemical analysis of formalin-fixed, paraffin-embedded IMR-90 cells labeling alpha smooth muscle Actin with ab265588 at 1/100 dilution. Detection: DAB.
-
Anti-alpha smooth muscle Actin antibody (ab265588)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab265588 has been referenced in 4 publications.
- Sánchez-Gloria JL et al. Anti-Inflammatory Effect of Allicin Associated with Fibrosis in Pulmonary Arterial Hypertension. Int J Mol Sci 22:N/A (2021). PubMed: 34445305
- Sui H et al. LINC00028 regulates the development of TGFß1-treated human tenon capsule fibroblasts by targeting miR-204-5p. Biochem Biophys Res Commun N/A:N/A (2020). PubMed: 32085895
- Guo Y et al. SIRT1-modified human umbilical cord mesenchymal stem cells ameliorate experimental peritoneal fibrosis by inhibiting the TGF-ß/Smad3 pathway. Stem Cell Res Ther 11:362 (2020). PubMed: 32811535
- Arellano-Buendía AS et al. Effects of Allicin on Pathophysiological Mechanisms during the Progression of Nephropathy Associated to Diabetes. Antioxidants (Basel) 9:N/A (2020). PubMed: 33203103