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  1. Link

    products/primary-antibodies/alpha-smooth-muscle-actin-antibody-blr082g-ab265588.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin
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Recombinant

Recombinant Anti-alpha smooth muscle Actin antibody [BLR082G] (ab265588)

  • Datasheet
  • SDS
Submit a review Submit a question References (4)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab265588)
  • Western blot - Anti-alpha smooth muscle Actin antibody (ab265588)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab265588)
  • Immunocytochemistry - Anti-alpha smooth muscle Actin antibody (ab265588)
  • Anti-alpha smooth muscle Actin antibody (ab265588)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BLR082G] to alpha smooth muscle Actin
  • Suitable for: WB, IHC-P, ICC
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-alpha smooth muscle Actin antibody [BLR082G]
    See all alpha smooth muscle Actin primary antibodies
  • Description

    Rabbit monoclonal [BLR082G] to alpha smooth muscle Actin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human alpha smooth muscle Actin aa 1-50. The exact sequence is proprietary. NP_001135417.1
    Database link: P62736

  • Positive control

    • IHC-P: Human breast tissue. Human colon carcinoma tissue. ICC: IMR-90 cells. WB: IMR-90 and NIH/3T3 whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.8
    Preservative: 0.09% Sodium azide
    Constituents: 98% Borate buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Purified from TCS.
  • Clonality

    Monoclonal
  • Clone number

    BLR082G
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin
    • Stem Cells
    • Lineage Markers
    • Mesoderm
    • Stem Cells
    • Mesenchymal Stem Cells
    • Myogenesis
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Actins
    • Cardiovascular
    • Vasculature
    • Vasculature Markers
    • Arterial
    • Cardiovascular
    • Vasculature
    • Smooth muscle cell (SMC)
    • Cytoskeleton
    • Developmental Biology
    • Lineage specification
    • Mesoderm
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Smooth Muscle Cells

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Positive Controls

    • NIH/3T3 whole cell lysate (ab7179)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab265588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 42 kDa.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC
1/100 - 1/500.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Notes
WB
1/1000. Predicted molecular weight: 42 kDa.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC
1/100 - 1/500.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
  • Sequence similarities

    Belongs to the actin family.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P62736 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 59 Human
    • Entrez Gene: 11475 Mouse
    • Omim: 102620 Human
    • SwissProt: P62736 Human
    • SwissProt: P62737 Mouse
    • Unigene: 500483 Human
    • Unigene: 213025 Mouse
    • Alternative names

      • a actin antibody
      • AAT6 antibody
      • ACTA_HUMAN antibody
      • ACTA2 antibody
      • Actin alpha 2 smooth muscle aorta antibody
      • Actin aortic smooth muscle antibody
      • Actin, aortic smooth muscle antibody
      • ACTSA antibody
      • ACTVS antibody
      • Alpha 2 actin antibody
      • Alpha actin 2 antibody
      • Alpha cardiac actin antibody
      • alpha sma antibody
      • Alpha-actin-2 antibody
      • Cell growth inhibiting gene 46 protein antibody
      • Cell growth-inhibiting gene 46 protein antibody
      • GIG46 antibody
      • Growth inhibiting gene 46 antibody
      • MYMY5 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab265588)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab265588)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling alpha smooth muscle Actin with ab265588 at 1/100 dilution. Detection: DAB.

    • Western blot - Anti-alpha smooth muscle Actin antibody (ab265588)
      Western blot - Anti-alpha smooth muscle Actin antibody (ab265588)
      All lanes : Anti-alpha smooth muscle Actin antibody [BLR082G] (ab265588) at 1/1000 dilution

      Lane 1 : MCF7 whole cell lysate at 50 µg
      Lane 2 : A549 whole cell lysate at 50 µg
      Lane 3 : Jurkat whole cell lysate at 50 µg
      Lane 4 : IMR-90 whole cell lysate at 10 µg
      Lane 5 : HepG2 whole cell lysate at 50 µg
      Lane 6 : LNCaP whole cell lysate at 50 µg
      Lane 7 : RKO whole cell lysate at 50 µg
      Lane 8 : K562 whole cell lysate at 50 µg
      Lane 9 : NIH/3T3 whole cell lysate at 50 µg
      Lane 10 : TCMK whole cell lysate at 50 µg

      Predicted band size: 42 kDa


      Exposure time: 30 seconds


      Cells prepared using NETN lysis buffer.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab265588)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab265588)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast tissue labeling alpha smooth muscle Actin with ab265588 at 1/100 dilution. Detection: DAB.

    • Immunocytochemistry - Anti-alpha smooth muscle Actin antibody (ab265588)
      Immunocytochemistry - Anti-alpha smooth muscle Actin antibody (ab265588)

      Immunocytochemical analysis of formalin-fixed, paraffin-embedded IMR-90 cells labeling alpha smooth muscle Actin with ab265588 at 1/100 dilution. Detection: DAB.

    • Anti-alpha smooth muscle Actin antibody (ab265588)
      Anti-alpha smooth muscle Actin antibody (ab265588)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (4)

    Publishing research using ab265588? Please let us know so that we can cite the reference in this datasheet.

    ab265588 has been referenced in 4 publications.

    • Sánchez-Gloria JL  et al. Anti-Inflammatory Effect of Allicin Associated with Fibrosis in Pulmonary Arterial Hypertension. Int J Mol Sci 22:N/A (2021). PubMed: 34445305
    • Sui H  et al. LINC00028 regulates the development of TGFß1-treated human tenon capsule fibroblasts by targeting miR-204-5p. Biochem Biophys Res Commun N/A:N/A (2020). PubMed: 32085895
    • Guo Y  et al. SIRT1-modified human umbilical cord mesenchymal stem cells ameliorate experimental peritoneal fibrosis by inhibiting the TGF-ß/Smad3 pathway. Stem Cell Res Ther 11:362 (2020). PubMed: 32811535
    • Arellano-Buendía AS  et al. Effects of Allicin on Pathophysiological Mechanisms during the Progression of Nephropathy Associated to Diabetes. Antioxidants (Basel) 9:N/A (2020). PubMed: 33203103

    Customer reviews and Q&As

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