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  1. Link

    products/primary-antibodies/alpha-tubulin-acetyl-k40-antibody-6-11b-1-ab24610.pdf

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Tags & Cell Markers Subcellular Markers Cytoskeleton Microtubules
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Anti-alpha Tubulin (acetyl K40) antibody [6-11B-1] (ab24610)

  • Datasheet
  • SDS
Reviews (33)Q&A (2)References (163)

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Western blot - Anti-alpha Tubulin (acetyl K40) antibody [6-11B-1] (ab24610)
  • Immunocytochemistry - Anti-alpha Tubulin (acetyl K40) antibody [6-11B-1] (ab24610)
  • Immunocytochemistry - Anti-alpha Tubulin (acetyl K40) antibody [6-11B-1] (ab24610)
  • Immunocytochemistry - Anti-alpha Tubulin (acetyl K40) antibody [6-11B-1] (ab24610)
  • Flow Cytometry - Anti-alpha Tubulin (acetyl K40) antibody [6-11B-1] (ab24610)

Key features and details

  • Mouse monoclonal [6-11B-1] to alpha Tubulin (acetyl K40)
  • Suitable for: Flow Cyt, ICC, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-alpha Tubulin (acetyl K40) antibody [6-11B-1]
    See all alpha Tubulin primary antibodies
  • Description

    Mouse monoclonal [6-11B-1] to alpha Tubulin (acetyl K40)
  • Host species

    Mouse
  • Specificity

    ab24610 detects acetylated alpha tubulin.
  • Tested applications

    Suitable for: Flow Cyt, ICC, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Tissue, cells or virus corresponding to alpha Tubulin (acetyl K40).

  • Epitope

    The antibody recognizes an epitope located on the a3 isoform of Chlamydomonas axonemal a-tubulin, within four residues of Lys40 when this amino acid is acetylated.
  • Positive control

    • In Western Blot, this antibody gave a positive signal in mouse brain tissue lysate and in the following whole cell lysates: HeLa; NIH3T3; PC12.
  • General notes

    Production of this antibody has been changed on 8th April 2016. This antibody is now purified from tissue culture supernatant. This shouldn’t affect the use of this antibody but if you have any issues, please contact our Scientific Support team.

    This antibody binds to primary cilia, centrioles, mitotic spindles, midbodies and to subsets of cytoplasmic microtubules in 3T3 and HeLa cells.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Proprietary Purification
  • Purification notes

    Purified from Tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    6-11B-1
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • Positive Controls

    • NIH/3T3 nuclear extract lysate (ab14874)
    • NIH/3T3 whole cell lysate (ab7179)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • alpha Tubulin peptide (ab195584)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab24610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use 1µl for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ICC
Use at an assay dependent concentration.
WB (12)
Use a concentration of 0.03 - 0.06 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
Notes
Flow Cyt
Use 1µl for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ICC
Use at an assay dependent concentration.
WB
Use a concentration of 0.03 - 0.06 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Sequence similarities

    Belongs to the tubulin family.
  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P68366 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7277 Human
    • Entrez Gene: 22145 Mouse
    • Entrez Gene: 316531 Rat
    • Omim: 191110 Human
    • SwissProt: P68366 Human
    • SwissProt: P68368 Mouse
    • SwissProt: Q5XIF6 Rat
    • Unigene: 75318 Human
    • Unigene: 1155 Mouse
    • Unigene: 92961 Rat
    see all
  • Alternative names

    • Alpha-tubulin 1 antibody
    • ALS22 antibody
    • B ALPHA 1 antibody
    • bA408E5.3 antibody
    • H2 ALPHA antibody
    • Hum a tub1 antibody
    • Hum a tub2 antibody
    • LIS3 antibody
    • MGC171407 antibody
    • MGC55332 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • tuba1l antibody
    • Tuba4a antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha antibody
    • Tubulin alpha-1 chain antibody
    • tubulin alpha-1B chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    • Tubulin, alpha 1 (testis specific) antibody
    • tubulin, alpha 1, like antibody
    • Tubulin, alpha 4a antibody
    • Tubulin, alpha, testis-specific antibody
    • Tubulin, alpha-1 antibody
    see all

Images

  • Western blot - Anti-alpha Tubulin (acetyl K40) antibody [6-11B-1] (ab24610)
    Western blot - Anti-alpha Tubulin (acetyl K40) antibody [6-11B-1] (ab24610)
    All lanes : Anti-alpha Tubulin (acetyl K40) antibody [6-11B-1] (ab24610) at 5 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 4 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa
    Additional bands at: 140 kDa, 25 kDa, 35 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 150 seconds
  • Immunocytochemistry - Anti-alpha Tubulin (acetyl K40) antibody [6-11B-1] (ab24610)
    Immunocytochemistry - Anti-alpha Tubulin (acetyl K40) antibody [6-11B-1] (ab24610)This image is courtesy of an Abreview submitted by Aaron Halpen

    ab24610 staining Acetylated alpha Tubulin in monkey kidney cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 3% PFA + 0.1% GA and blocked with 3% BSA + 0.5% Triton X-100 for 45 minutes at 25°C. Samples were incubated with primary antibody (1/100 in 3% BSA + 0.5% Triton X-100) for 1 hour at 21°C. An Alexa Fluor® 647-conjugated donkey anti-rabbit IgG polyclonal (2 µg/ml) was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry - Anti-alpha Tubulin (acetyl K40) antibody [6-11B-1] (ab24610)
    Immunocytochemistry - Anti-alpha Tubulin (acetyl K40) antibody [6-11B-1] (ab24610)

    ICC/IF image of ab24610 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab24610, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iT™ FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • Immunocytochemistry - Anti-alpha Tubulin (acetyl K40) antibody [6-11B-1] (ab24610)
    Immunocytochemistry - Anti-alpha Tubulin (acetyl K40) antibody [6-11B-1] (ab24610)

    ICC/IF image of ab24610 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24610, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Flow Cytometry - Anti-alpha Tubulin (acetyl K40) antibody [6-11B-1] (ab24610)
    Flow Cytometry - Anti-alpha Tubulin (acetyl K40) antibody [6-11B-1] (ab24610)

    Overlay histogram showing HeLa cells stained with ab24610 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24610, 1µg/1x106 cells) for 30 min at 22ºC. (This data was generated from a purified version of the antibody. Some lots are produced as ascites fluid. We suggest 1µl/1x106 cells for ascites preparations). The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Protocols

  • Flow cytometry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (163)

Publishing research using ab24610? Please let us know so that we can cite the reference in this datasheet.

ab24610 has been referenced in 163 publications.

  • Li C  et al. SRPS associated protein WDR60 regulates the multipolar-to-bipolar transition of migrating neurons during cortical development. Cell Death Dis 12:75 (2021). PubMed: 33436552
  • Wang H  et al. Mettl14-mediated m6A modification modulates neuron apoptosis during the repair of spinal cord injury by regulating the transformation from pri-mir-375 to miR-375. Cell Biosci 11:52 (2021). PubMed: 33706799
  • Tambutté E  et al. A role for primary cilia in coral calcification? Cell Tissue Res 383:1093-1102 (2021). PubMed: 33330957
  • Hu CS  et al. Lentivirus-mediated silencing of CNTN1 enhances gefitinib sensitivity by reversing epithelial-mesenchymal transition in lung adenocarcinoma A549 cells. Oncol Lett 21:433 (2021). PubMed: 33868471
  • Dihazi H  et al. PAC-Mediated AKI Protection Is Critically Mediated but Does Not Exclusively Depend on Cell-Derived Microvesicles. Int J Nephrol 2021:8864183 (2021). PubMed: 33777453
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 35 Abreviews or Q&A

Western blot abreview for Anti-alpha Tubulin (acetyl K40) antibody [6-11B-1]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - other (hek293)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
50 µg
Specification
hek293
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Robert Kupp

Verified customer

Submitted Oct 19 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Tubulin (acetyl K40) antibody [6-11B-1]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (DU145 Prostate cancer cell line)
Permeabilization
Yes - 0.1% Triton X-100
Specification
DU145 Prostate cancer cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Dimitra Kalamida

Verified customer

Submitted Dec 28 2019

Western blot abreview for Anti-alpha Tubulin (acetyl K40) antibody [6-11B-1]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (C42B Prostate cancer cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
C42B Prostate cancer cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Dimitra Kalamida

Verified customer

Submitted Dec 25 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Tubulin (acetyl K40) antibody [6-11B-1]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
No
Specification
HeLa
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Methanol
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 15 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Tubulin (acetyl K40) antibody [6-11B-1]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (C22 mouse sciatic nerve paraffin-embedded section)
Permeabilization
No
Specification
C22 mouse sciatic nerve paraffin-embedded section
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 22 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Tubulin (acetyl K40) antibody [6-11B-1]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HAP1)
Permeabilization
Yes - 0.5% Triton X-100, 5min.
Specification
HAP1
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 13 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Tubulin (acetyl K40) antibody [6-11B-1]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (immortalised bone marrow-derived macrophage)
Permeabilization
Yes - 0.1% Triton X-100
Specification
immortalised bone marrow-derived macrophage
Blocking step
No blocking step used for 1 hour(s) and 0 minute(s) · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. James Harris

Verified customer

Submitted Jun 02 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-Acetylated alpha Tubulin antibody [6-11B-1]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (Swine Testicle Cells (ST))
Permeabilization
Yes - 0.1% Triton X-100
Specification
Swine Testicle Cells (ST)
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 28 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-Acetylated alpha Tubulin antibody [6-11B-1]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Fibroblasts)
Permeabilization
Yes - PBS1X, 0.2% triton
Specification
Fibroblasts
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 29 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-Acetylated alpha Tubulin antibody [6-11B-1]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
3% BSA and 0.5% TX100 as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: 25°C
Sample
Monkey Cell (Kidney)
Specification
Kidney
Permeabilization
No
Fixative
3% PFA and 0.1% GA
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Aaron Halpen

Verified customer

Submitted Sep 29 2014

1-10 of 35 Abreviews or Q&A

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