Anti-alpha Tubulin antibody [B-5-1-2] (ab11304)
![Immunocytochemistry - Anti-alpha Tubulin antibody [B-5-1-2] (ab11304)](https://www.abcam.com/ps/products/11/ab11304/Images/ab11304-209528-anti-alpha-tubulin-antibody-b-5-1-2-immunofluorescence.jpg "Immunocytochemistry - Anti-alpha Tubulin antibody [B-5-1-2] (ab11304)")
Key features and details
- Mouse monoclonal [B-5-1-2] to alpha Tubulin
- Suitable for: ICC
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-alpha Tubulin antibody [B-5-1-2]
See all alpha Tubulin primary antibodies -
Description
Mouse monoclonal [B-5-1-2] to alpha Tubulin -
Host species
Mouse -
Tested applications
Suitable for: ICCmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Dog, Pig, Xenopus laevis, Cynomolgus monkey, African green monkey, Xenopus tropicalis
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Immunogen
Full length native protein (purified) corresponding to alpha Tubulin. Sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.
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Epitope
The antibody recognizes an epitope located at the C-terminal end of the alpha tubulin isoform. -
Positive control
- 293T whole cell lysate (ab95494) can be used as a positive control in WB. Total cell extract of the Human foreskin fibroblast cell line (FS11).
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General notes
Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
B-5-1-2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11304 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
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| Notes |
|---|
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ICC
Use at an assay dependent concentration. |
Target
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Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
Sequence similarities
Belongs to the tubulin family. -
Post-translational
modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 7277 Human
- Omim: 191110 Human
- SwissProt: P68366 Human
- Unigene: 75318 Human
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Alternative names
- Alpha-tubulin 1 antibody
- ALS22 antibody
- B ALPHA 1 antibody
see all
Images
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Immunocytochemistry - Anti-alpha Tubulin antibody [B-5-1-2] (ab11304)
ab11304 staining alpha Tubulin in the HEK293T cell line by ICC/IF (Immunocytochemistry/immunofluorescence) transfected with a recombinant protein HA tagged dual stained with Anti-HA Mouse primary antibody and Anti-Mouse Alexa Fluor®543 secondary antibody. Microtubules detected with Anti-α-tubulin Mouse primary antibody and Anti-Mouse Alexa Fluor®488 secondary antibody.Nuclus visualised with DAPI.
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![Immunocytochemistry - Anti-alpha Tubulin antibody [B-5-1-2] (ab11304)](https://www.abcam.com/ps/products/11/ab11304/Images/ab11304-18874-anti-alpha-tubulin-antibody-b-5-1-2-immunofluorescence.jpg)
Immunocytochemistry - Anti-alpha Tubulin antibody [B-5-1-2] (ab11304)This image is courtesy of an Anonymous Abreviewab11304 at a 1/100 dilution staining human HeLa cells by Immunocytochemistry/Immunofluorescence. The cells were methanol fixed and blocked for 1 hour with 10% serum. The antibody was then incubated with the cells for 1 hour. Bound antibody was detected using a Goat anti-mouse TRITC antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (45)
ab11304 has been referenced in 45 publications.
- Spena S et al. Development of a Specific Monoclonal Antibody to Detect Male Cells Expressing the RPS4Y1 Protein. Int J Mol Sci 22:N/A (2021). PubMed: 33670450
- Kuo EY & Lee TM Molecular Mechanisms Underlying the Acclimation of Chlamydomonas reinhardtii Against Nitric Oxide Stress. Front Plant Sci 12:690763 (2021). PubMed: 34421944
- Wang Y et al. LGR4, Not LGR5, Enhances hPSC Hematopoiesis by Facilitating Mesoderm Induction via TGF-Beta Signaling Activation. Cell Rep 31:107600 (2020). PubMed: 32375050
- Kochi C et al. Simulated vehicle exhaust exposure (SVEE) in rats impairs renal mitochondrial function. Clin Exp Hypertens 42:571-579 (2020). PubMed: 32423257
- Kuo EY et al. High Light-Induced Nitric Oxide Production Induces Autophagy and Cell Death in Chlamydomonas reinhardtii. Front Plant Sci 11:772 (2020). PubMed: 32587598