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    products/primary-antibodies/alpha-tubulin-antibody-epr13478b-loading-control-ab176560.pdf

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RecombinantRabMAb

Recombinant Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

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  • SDS
Reviews (11) Submit a question References (20)

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Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
  • Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
  • Flow Cytometry (Intracellular) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
  • Flow Cytometry (Intracellular) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
  • Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13478(B)] to alpha Tubulin - Loading Control
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free HRP PE

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Overview

  • Product name

    Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control
    See all alpha Tubulin primary antibodies
  • Description

    Rabbit monoclonal [EPR13478(B)] to alpha Tubulin - Loading Control
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, African green monkey
  • Immunogen

    Synthetic peptide within Human alpha Tubulin aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P68366

  • Positive control

    • HeLa, Jurkat, A431 and K562 cell lysates; Human kidney and uterus tissues; A431 and Jurkat cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13478(B)
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin
    • Isotype/Loading Controls
    • Loading Controls
    • Alpha Tubulin
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • HRP Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab185067)
    • Alexa Fluor® 405 Anti-alpha Tubulin antibody [EPR13478(B)] (ab210167)
    • Anti-alpha Tubulin antibody [EPR13478(B)] - BSA and Azide free (ab220805)
    • Alexa Fluor® 647 Anti-alpha Tubulin antibody [EPR13478(B)] (ab225254)
    • PE Anti-alpha Tubulin antibody [EPR13478(B)] (ab225255)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab7899)
    • A-431 whole cell lysate (ab7909)
    • K-562 whole cell lysate (ab7911)
  • Related Products

    • alpha Tubulin peptide (ab195584)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab176560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/60.

For unpurified, use 1/10.  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (8)
1/2000 - 1/10000. Predicted molecular weight: 50 kDa.

For unpurified, use 1/1000 - 1/10000.

IHC-P
1/350. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified, use 1/100.

ICC/IF (2)
1/350.

For unpurified, use 1/100.

Notes
Flow Cyt (Intra)
1/60.

For unpurified, use 1/10.  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/2000 - 1/10000. Predicted molecular weight: 50 kDa.

For unpurified, use 1/1000 - 1/10000.

IHC-P
1/350. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified, use 1/100.

ICC/IF
1/350.

For unpurified, use 1/100.

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Sequence similarities

    Belongs to the tubulin family.
  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P68366 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7277 Human
    • Entrez Gene: 22145 Mouse
    • Entrez Gene: 316531 Rat
    • Omim: 191110 Human
    • SwissProt: P68366 Human
    • SwissProt: P68368 Mouse
    • SwissProt: Q5XIF6 Rat
    • Unigene: 75318 Human
    • Unigene: 1155 Mouse
    • Unigene: 92961 Rat
    see all
  • Alternative names

    • Alpha-tubulin 1 antibody
    • ALS22 antibody
    • B ALPHA 1 antibody
    • bA408E5.3 antibody
    • H2 ALPHA antibody
    • Hum a tub1 antibody
    • Hum a tub2 antibody
    • LIS3 antibody
    • MGC171407 antibody
    • MGC55332 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • tuba1l antibody
    • Tuba4a antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha antibody
    • Tubulin alpha-1 chain antibody
    • tubulin alpha-1B chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    • Tubulin, alpha 1 (testis specific) antibody
    • tubulin, alpha 1, like antibody
    • Tubulin, alpha 4a antibody
    • Tubulin, alpha, testis-specific antibody
    • Tubulin, alpha-1 antibody
    see all

Images

  • Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
    Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
    Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560) at 1/7000 dilution (Purified) + Jurkat cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus tissue labeling alpha Tubulin with unpurified ab176560 at a 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

    Immunohistochemical staining of paraffin embedded human kidney with purified ab176560 at a dilution of 1/350. A prediluted HRP polymer for rabbit IgG was used as the secondary and the sample was counter stained with hematoxylin. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

    Immunofluorescent staining of A431 cells fixed in 4% PFA with purified ab176560 at a dilution of 1/350. An Alexa Fluor® 555 goat anti-rabbit was used as the secondary at a dilution of 1/500 and the sample was counter stained with DAPI. An Alexa Fluor® 555 goat anti-mouse was used at a dilution of 1/500 as the negative control and is shown in the bottom right hand panel.

  • Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
    Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
    All lanes : Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560) at 1/1000 dilution (Unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 50 kDa



    Secondary antibodies - anti-rabbit HRP (ab6721)

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

    Immunofluorescent staining of A431 cells fixed in 4% PFA with unpurified ab176560 at a dilution of 1/100. An Alexa Fluor® 555 goat anti-rabbit was used as the secondary at a dilution of 1/500 and the sample was counter stained with DAPI. An Alexa Fluor® 555 goat anti-mouse was used at a dilution of 1/500 as the negative control and is shown in the bottom right hand panel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

    Immunohistochemical staining of paraffin embedded human kidney with unpurified ab176560 at a dilution of 1/100. A prediluted HRP polymer for rabbit IgG was used as the secondary and the sample was counter stained with hematoxylin. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
    Flow Cytometry (Intracellular) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

    Intracellular Flow Cytometry analysis of permeabilized HeLa cells labeling alpha Tubulin (pink) with purifiedab176560 at a 1/70 dilution, or negative control rabbit IgG (green). The secondary antibody was FITC goat anti-rabbit. 

     

     

  • Flow Cytometry (Intracellular) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
    Flow Cytometry (Intracellular) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

    Intracellular Flow Cytometry analysis of permeabilized Jurkat cells labeling alpha Tubulin (red) with unpurified ab176560 at a 1/10 dilution, or negative control rabbit IgG (green) 

     

     

  • Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
    Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (20)

Publishing research using ab176560? Please let us know so that we can cite the reference in this datasheet.

ab176560 has been referenced in 20 publications.

  • Fujita Y  et al. Angiotensin II type 1a receptor loss ameliorates chronic tubulointerstitial damage after renal ischemia reperfusion. Sci Rep 11:982 (2021). PubMed: 33441837
  • Schwich OD  et al. SRSF3 and SRSF7 modulate 3'UTR length through suppression or activation of proximal polyadenylation sites and regulation of CFIm levels. Genome Biol 22:82 (2021). PubMed: 33706811
  • Zhang H  et al. CircLIFR synergizes with MSH2 to attenuate chemoresistance via MutSa/ATM-p73 axis in bladder cancer. Mol Cancer 20:70 (2021). PubMed: 33874956
  • Sonawane SK & Chinnathambi S Epigallocatechin-3-gallate modulates Tau Post-translational modifications and cytoskeletal network. Oncotarget 12:1083-1099 (2021). PubMed: 34084282
  • Kanellis DC  et al. The exon-junction complex helicase eIF4A3 controls cell fate via coordinated regulation of ribosome biogenesis and translational output. Sci Adv 7:N/A (2021). PubMed: 34348895
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 11 Abreviews or Q&A

Western blot abreview for Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse lung homogenate)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Mouse lung homogenate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Helen Stoelting

Verified customer

Submitted Jan 28 2022

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse vascular smooth muscle cells)
Permeabilization
Yes - NP40
Specification
Mouse vascular smooth muscle cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 11 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - NP40
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 11 2021

Western blot abreview for Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse vascular smooth muscle cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Mouse vascular smooth muscle cells
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 09 2021

Western blot abreview for Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
U2OS
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 11 2021

Western blot abreview for Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control

Inconclusive
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Caenorhabditis elegans Tissue lysate - whole (Whole worm lysate (U2OS cells as positive control))
Gel Running Conditions
Reduced Denaturing (Bis-Tris 4%-12%)
Loading amount
5 µg
Specification
Whole worm lysate (U2OS cells as positive control)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MS. Sonia Ravanelli

Verified customer

Submitted Oct 28 2020

Immunohistochemistry (Frozen sections) abreview for Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control

Below Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis, adult)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, adult
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Bryan Niedenberger

Verified customer

Submitted Aug 08 2016

Western blot abreview for Anti-alpha Tubulin [EPR13478(B)] antibody - Loading Control

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (13%)
Sample
Human Cell lysate - whole cell (HCT116 and MEF cell lysates)
Specification
HCT116 and MEF cell lysates
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Christian Marx

Verified customer

Submitted Jan 23 2014

Western blot abreview for Anti-alpha Tubulin [EPR13478(B)] antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (RAMOS cells)
Specification
RAMOS cells
Treatment
TGF-beta, 24 hours, 50 pM or untreated
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 04 2013

Western blot abreview for Anti-alpha Tubulin [EPR13478(B)] antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
African Green Monkey Cell lysate - whole cell (COS-1 cells)
Specification
COS-1 cells
Treatment
TGF-beta, 24 hours, 50 pM or untreated
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 04 2013

1-10 of 11 Abreviews or Q&A

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