Name: Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control
Brand: RabMAb
SKU: ab176560
Rating: 5 (11 reviews)

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Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13478(B)] to alpha Tubulin - Loading Control
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
Reacts with: Human

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free HRP PE

Product name

Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control
See all alpha Tubulin primary antibodies
Description

Rabbit monoclonal [EPR13478(B)] to alpha Tubulin - Loading Control
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat, African green monkey
Immunogen

Synthetic peptide within Human alpha Tubulin aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P68366
Positive control
- HeLa, Jurkat, A431 and K562 cell lysates; Human kidney and uterus tissues; A431 and Jurkat cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13478(B)
Isotype

IgG
Research areas
- Neuroscience
- Processes

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab176560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/60. For unpurified, use 1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB	(8)	1/2000 - 1/10000. Predicted molecular weight: 50 kDa. For unpurified, use 1/1000 - 1/10000.
IHC-P		1/350. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified, use 1/100.
ICC/IF	(2)	1/350. For unpurified, use 1/100.

Notes
Flow Cyt (Intra) 1/60. For unpurified, use 1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/2000 - 1/10000. Predicted molecular weight: 50 kDa. For unpurified, use 1/1000 - 1/10000.
IHC-P 1/350. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified, use 1/100.
ICC/IF 1/350. For unpurified, use 1/100.

Function

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Sequence similarities

Belongs to the tubulin family.
Post-translational
modifications

Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
Cellular localization

Cytoplasm > cytoskeleton.
Target information above from: UniProt accession P68366 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 7277 Human
- Entrez Gene: 22145 Mouse
- Entrez Gene: 316531 Rat
- Omim: 191110 Human
- SwissProt: P68366 Human
- SwissProt: P68368 Mouse
- SwissProt: Q5XIF6 Rat
- Unigene: 75318 Human
- Unigene: 1155 Mouse
- Unigene: 92961 Rat
see all
Alternative names
- Alpha-tubulin 1 antibody
- ALS22 antibody
- B ALPHA 1 antibody
- bA408E5.3 antibody
- H2 ALPHA antibody
- Hum a tub1 antibody
- Hum a tub2 antibody
- LIS3 antibody
- MGC171407 antibody
- MGC55332 antibody
- TBA4A_HUMAN antibody
- Testis-specific alpha-tubulin antibody
- TUBA1 antibody
- TUBA1A antibody
- tuba1l antibody
- Tuba4a antibody
- Tubulin alpha 1 chain antibody
- Tubulin alpha antibody
- Tubulin alpha-1 chain antibody
- tubulin alpha-1B chain antibody
- Tubulin alpha-4A chain antibody
- Tubulin H2-alpha antibody
- Tubulin, alpha 1 (testis specific) antibody
- tubulin, alpha 1, like antibody
- Tubulin, alpha 4a antibody
- Tubulin, alpha, testis-specific antibody
- Tubulin, alpha-1 antibody
see all

Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560) at 1/7000 dilution (Purified) + Jurkat cell lysate at 10 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus tissue labeling alpha Tubulin with unpurified ab176560 at a 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

Immunohistochemical staining of paraffin embedded human kidney with purified ab176560 at a dilution of 1/350. A prediluted HRP polymer for rabbit IgG was used as the secondary and the sample was counter stained with hematoxylin. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

Immunofluorescent staining of A431 cells fixed in 4% PFA with purified ab176560 at a dilution of 1/350. An Alexa Fluor^® 555 goat anti-rabbit was used as the secondary at a dilution of 1/500 and the sample was counter stained with DAPI. An Alexa Fluor^® 555 goat anti-mouse was used at a dilution of 1/500 as the negative control and is shown in the bottom right hand panel.
Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

All lanes : Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560) at 1/1000 dilution (Unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : A431 cell lysate
Lane 4 : K562 cell lysate

Lysates/proteins at 10 µg per lane.

Developed using the ECL technique.

Predicted band size: 50 kDa

Secondary antibodies - anti-rabbit HRP (ab6721)
Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

Immunofluorescent staining of A431 cells fixed in 4% PFA with unpurified ab176560 at a dilution of 1/100. An Alexa Fluor^® 555 goat anti-rabbit was used as the secondary at a dilution of 1/500 and the sample was counter stained with DAPI. An Alexa Fluor^® 555 goat anti-mouse was used at a dilution of 1/500 as the negative control and is shown in the bottom right hand panel.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

Immunohistochemical staining of paraffin embedded human kidney with unpurified ab176560 at a dilution of 1/100. A prediluted HRP polymer for rabbit IgG was used as the secondary and the sample was counter stained with hematoxylin. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

Intracellular Flow Cytometry analysis of permeabilized HeLa cells labeling alpha Tubulin (pink) with purifiedab176560 at a 1/70 dilution, or negative control rabbit IgG (green). The secondary antibody was FITC goat anti-rabbit.
Flow Cytometry (Intracellular) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

Intracellular Flow Cytometry analysis of permeabilized Jurkat cells labeling alpha Tubulin (red) with unpurified ab176560 at a 1/10 dilution, or negative control rabbit IgG (green)
Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab176560? Please let us know so that we can cite the reference in this datasheet.

ab176560 has been referenced in 20 publications.

Fujita Y et al. Angiotensin II type 1a receptor loss ameliorates chronic tubulointerstitial damage after renal ischemia reperfusion. Sci Rep 11:982 (2021). PubMed: 33441837
Schwich OD et al. SRSF3 and SRSF7 modulate 3'UTR length through suppression or activation of proximal polyadenylation sites and regulation of CFIm levels. Genome Biol 22:82 (2021). PubMed: 33706811
Zhang H et al. CircLIFR synergizes with MSH2 to attenuate chemoresistance via MutSa/ATM-p73 axis in bladder cancer. Mol Cancer 20:70 (2021). PubMed: 33874956
Sonawane SK & Chinnathambi S Epigallocatechin-3-gallate modulates Tau Post-translational modifications and cytoskeletal network. Oncotarget 12:1083-1099 (2021). PubMed: 34084282
Kanellis DC et al. The exon-junction complex helicase eIF4A3 controls cell fate via coordinated regulation of ribosome biogenesis and translational output. Sci Adv 7:N/A (2021). PubMed: 34348895

View all Publications for this product

Customer reviews and Q&As

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1-10 of 11 Abreviews or Q&A

Application

Western blot

Sample

Mouse Tissue lysate - whole (Mouse lung homogenate)

Gel Running Conditions

Reduced Denaturing

Loading amount

30 µg

Specification

Mouse lung homogenate

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Helen Stoelting

Verified customer

Submitted Jan 28 2022

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (Mouse vascular smooth muscle cells)

Permeabilization

Yes - NP40

Specification

Mouse vascular smooth muscle cells

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 11 2021

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (U2OS)

Permeabilization

Yes - NP40

Specification

U2OS

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 11 2021

Application

Western blot

Sample

Mouse Cell lysate - whole cell (Mouse vascular smooth muscle cells)

Gel Running Conditions

Reduced Denaturing

Loading amount

30 µg

Specification

Mouse vascular smooth muscle cells

Blocking step

Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 09 2021

Application

Western blot

Sample

Human Cell lysate - whole cell (U2OS)

Gel Running Conditions

Reduced Denaturing

Loading amount

25 µg

Specification

U2OS

Blocking step

Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 11 2021

Application

Western blot

Sample

Caenorhabditis elegans Tissue lysate - whole (Whole worm lysate (U2OS cells as positive control))

Gel Running Conditions

Reduced Denaturing (Bis-Tris 4%-12%)

Loading amount

5 µg

Specification

Whole worm lysate (U2OS cells as positive control)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MS. Sonia Ravanelli

Verified customer

Submitted Oct 28 2020

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Testis, adult)

Permeabilization

Yes - 0.1% Triton X-100 in PBS

Specification

Testis, adult

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Bryan Niedenberger

Verified customer

Submitted Aug 08 2016

Application

Western blot

Loading amount

15 µg

Gel Running Conditions

Reduced Denaturing (13%)

Sample

Human Cell lysate - whole cell (HCT116 and MEF cell lysates)

Specification

HCT116 and MEF cell lysates

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Christian Marx

Verified customer

Submitted Jan 23 2014

Application

Western blot

Loading amount

20 µg

Gel Running Conditions

Reduced Denaturing

Sample

Human Cell lysate - whole cell (RAMOS cells)

Specification

RAMOS cells

Treatment

TGF-beta, 24 hours, 50 pM or untreated

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 04 2013

Application

Western blot

Loading amount

20 µg

Gel Running Conditions

Reduced Denaturing

Sample

African Green Monkey Cell lysate - whole cell (COS-1 cells)

Specification

COS-1 cells

Treatment

TGF-beta, 24 hours, 50 pM or untreated

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 04 2013

1-10 of 11 Abreviews or Q&A

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Related Products

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (20)

Customer reviews and Q&As

Western blot abreview for Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control

Western blot abreview for Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control

Western blot abreview for Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control

Western blot abreview for Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control

Immunohistochemistry (Frozen sections) abreview for Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control

Western blot abreview for Anti-alpha Tubulin [EPR13478(B)] antibody - Loading Control

Western blot abreview for Anti-alpha Tubulin [EPR13478(B)] antibody

Western blot abreview for Anti-alpha Tubulin [EPR13478(B)] antibody

Post-translational
modifications