Recombinant Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13478(B)] to alpha Tubulin - Loading Control
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control
See all alpha Tubulin primary antibodies -
Description
Rabbit monoclonal [EPR13478(B)] to alpha Tubulin - Loading Control -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, African green monkey -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, Jurkat, A431 and K562 cell lysates; Human kidney and uterus tissues; A431 and Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13478(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- HRP Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab185067)
- Alexa Fluor® 405 Anti-alpha Tubulin antibody [EPR13478(B)] (ab210167)
- Anti-alpha Tubulin antibody [EPR13478(B)] - BSA and Azide free (ab220805)
- Alexa Fluor® 647 Anti-alpha Tubulin antibody [EPR13478(B)] (ab225254)
- PE Anti-alpha Tubulin antibody [EPR13478(B)] (ab225255)
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/60.
For unpurified, use 1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB | (8) |
1/2000 - 1/10000. Predicted molecular weight: 50 kDa.
For unpurified, use 1/1000 - 1/10000. |
IHC-P |
1/350. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified, use 1/100. |
|
ICC/IF | (2) |
1/350.
For unpurified, use 1/100. |
Notes |
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Flow Cyt (Intra)
1/60. For unpurified, use 1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/2000 - 1/10000. Predicted molecular weight: 50 kDa. For unpurified, use 1/1000 - 1/10000. |
IHC-P
1/350. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified, use 1/100. |
ICC/IF
1/350. For unpurified, use 1/100. |
Target
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Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
Sequence similarities
Belongs to the tubulin family. -
Post-translational
modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 7277 Human
- Entrez Gene: 22145 Mouse
- Entrez Gene: 316531 Rat
- Omim: 191110 Human
- SwissProt: P68366 Human
- SwissProt: P68368 Mouse
- SwissProt: Q5XIF6 Rat
- Unigene: 75318 Human
see all -
Alternative names
- Alpha-tubulin 1 antibody
- ALS22 antibody
- B ALPHA 1 antibody
see all
Images
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Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560) at 1/7000 dilution (Purified) + Jurkat cell lysate at 10 µg
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus tissue labeling alpha Tubulin with unpurified ab176560 at a 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical staining of paraffin embedded human kidney with purified ab176560 at a dilution of 1/350. A prediluted HRP polymer for rabbit IgG was used as the secondary and the sample was counter stained with hematoxylin. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunofluorescent staining of A431 cells fixed in 4% PFA with purified ab176560 at a dilution of 1/350. An Alexa Fluor® 555 goat anti-rabbit was used as the secondary at a dilution of 1/500 and the sample was counter stained with DAPI. An Alexa Fluor® 555 goat anti-mouse was used at a dilution of 1/500 as the negative control and is shown in the bottom right hand panel.
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All lanes : Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560) at 1/1000 dilution (Unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : A431 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 50 kDaSecondary antibodies - anti-rabbit HRP (ab6721)
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Immunofluorescent staining of A431 cells fixed in 4% PFA with unpurified ab176560 at a dilution of 1/100. An Alexa Fluor® 555 goat anti-rabbit was used as the secondary at a dilution of 1/500 and the sample was counter stained with DAPI. An Alexa Fluor® 555 goat anti-mouse was used at a dilution of 1/500 as the negative control and is shown in the bottom right hand panel.
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Immunohistochemical staining of paraffin embedded human kidney with unpurified ab176560 at a dilution of 1/100. A prediluted HRP polymer for rabbit IgG was used as the secondary and the sample was counter stained with hematoxylin. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Intracellular Flow Cytometry analysis of permeabilized HeLa cells labeling alpha Tubulin (pink) with purifiedab176560 at a 1/70 dilution, or negative control rabbit IgG (green). The secondary antibody was FITC goat anti-rabbit.
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Intracellular Flow Cytometry analysis of permeabilized Jurkat cells labeling alpha Tubulin (red) with unpurified ab176560 at a 1/10 dilution, or negative control rabbit IgG (green)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (32)
ab176560 has been referenced in 32 publications.
- Wachholz V et al. Inhibitors of class I HDACs and of FLT3 combine synergistically against leukemia cells with mutant FLT3. Arch Toxicol 96:177-193 (2022). PubMed: 34665271
- Hewitt CS et al. Rational Development and Characterization of a Ubiquitin Variant with Selectivity for Ubiquitin C-Terminal Hydrolase L3. Biomolecules 12:N/A (2022). PubMed: 35053210
- Tai HC et al. Melatonin suppresses the metastatic potential of osteoblastic prostate cancers by inhibiting integrin α2 β1 expression. J Pineal Res 72:e12793 (2022). PubMed: 35174530
- Cioce M et al. Butein-instigated miR-186-5p-dependent modulation of TWIST1 affects resistance to cisplatin and bioenergetics of Malignant Pleural Mesothelioma cells. Cancer Drug Resist 5:814-828 (2022). PubMed: 36176750
- Huang L et al. AZD6738 Inhibits fibrotic response of conjunctival fibroblasts by regulating checkpoint kinase 1/P53 and PI3K/AKT pathways. Front Pharmacol 13:990401 (2022). PubMed: 36204234