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  1. Link

    products/primary-antibodies/alpha-tubulin-antibody-epr13799-ab184970.pdf

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Tags & Cell Markers Subcellular Markers Cytoskeleton Microtubules
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RecombinantRabMAb

Recombinant Anti-alpha Tubulin antibody [EPR13799] (ab184970)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question References (7)

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Western blot - Anti-alpha Tubulin antibody [EPR13799] (ab184970)
  • Anti-alpha Tubulin antibody [EPR13799] (ab184970)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13799] to alpha Tubulin
  • Suitable for: WB
  • Reacts with: Saccharomyces cerevisiae

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Overview

  • Product name

    Anti-alpha Tubulin antibody [EPR13799]
    See all alpha Tubulin primary antibodies
  • Description

    Rabbit monoclonal [EPR13799] to alpha Tubulin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Saccharomyces cerevisiae extract.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13799
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin
    • Isotype/Loading Controls
    • Loading Controls
    • Alpha Tubulin
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-alpha Tubulin antibody [EPR13799] - BSA and Azide free (ab232651)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab184970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).
Notes
WB
1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Sequence similarities

    Belongs to the tubulin family.
  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P68366 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Alpha-tubulin 1 antibody
    • ALS22 antibody
    • B ALPHA 1 antibody
    • bA408E5.3 antibody
    • H2 ALPHA antibody
    • Hum a tub1 antibody
    • Hum a tub2 antibody
    • LIS3 antibody
    • MGC171407 antibody
    • MGC55332 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • tuba1l antibody
    • Tuba4a antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha antibody
    • Tubulin alpha-1 chain antibody
    • tubulin alpha-1B chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    • Tubulin, alpha 1 (testis specific) antibody
    • tubulin, alpha 1, like antibody
    • Tubulin, alpha 4a antibody
    • Tubulin, alpha, testis-specific antibody
    • Tubulin, alpha-1 antibody
    see all

Images

  • Western blot - Anti-alpha Tubulin antibody [EPR13799] (ab184970)
    Western blot - Anti-alpha Tubulin antibody [EPR13799] (ab184970)
    Anti-alpha Tubulin antibody [EPR13799] (ab184970) at 1/50000 dilution + Saccharomyces cerevisiae extract at 10 µg

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?

  • Anti-alpha Tubulin antibody [EPR13799] (ab184970)
    Anti-alpha Tubulin antibody [EPR13799] (ab184970)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (7)

Publishing research using ab184970? Please let us know so that we can cite the reference in this datasheet.

ab184970 has been referenced in 7 publications.

  • Wang Y  et al. Noncanonical regulation of phosphatidylserine metabolism by a Sec14-like protein and a lipid kinase. J Cell Biol 219:N/A (2020). PubMed: 32303746
  • Li X  et al. Toll interacting protein protects bronchial epithelial cells from bleomycin-induced apoptosis. FASEB J 34:9884-9898 (2020). PubMed: 32596871
  • Chan YH  et al. An allosteric pathway explains beneficial fitness in yeast for long-range mutations in an essential TIM barrel enzyme. Protein Sci 29:1911-1923 (2020). PubMed: 32643222
  • Renz C  et al. Ubc13-Mms2 cooperates with a family of RING E3 proteins in budding yeast membrane protein sorting. J Cell Sci 133:N/A (2020). PubMed: 32265276
  • Corcoles-Saez I  et al. Functional link between mitochondria and Rnr3, the minor catalytic subunit of yeast ribonucleotide reductase. Microb Cell 6:286-294 (2019). PubMed: 31172013
  • Kwasna D  et al. Discovery and Characterization of ZUFSP/ZUP1, a Distinct Deubiquitinase Class Important for Genome Stability. Mol Cell 70:150-164.e6 (2018). PubMed: 29576527
  • Ding MZ  et al. Biosynthesis of Taxadiene in Saccharomyces cerevisiae : selection of geranylgeranyl diphosphate synthase directed by a computer-aided docking strategy. PLoS One 9:e109348 (2014). WB . PubMed: 25295588

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