Recombinant Anti-alpha Tubulin antibody [EPR13799] (ab184970)
![Western blot - Anti-alpha Tubulin antibody [EPR13799] (ab184970)](https://www.abcam.com/ps/products/184/ab184970/Images/ab184970-217769-anti-alpha-tubulin-antibody-epr13799-western-blot.jpg "Western blot - Anti-alpha Tubulin antibody [EPR13799] (ab184970)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13799] to alpha Tubulin
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
Related conjugates and formulations
Overview
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Product name
Anti-alpha Tubulin antibody [EPR13799]
See all alpha Tubulin primary antibodies -
Description
Rabbit monoclonal [EPR13799] to alpha Tubulin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Saccharomyces cerevisiae extract.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13799 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).
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| Notes |
|---|
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WB
1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa). |
Target
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Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
Sequence similarities
Belongs to the tubulin family. -
Post-translational
modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Alternative names
- Alpha-tubulin 1 antibody
- ALS22 antibody
- B ALPHA 1 antibody
see all
Images
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Western blot - Anti-alpha Tubulin antibody [EPR13799] (ab184970)Anti-alpha Tubulin antibody [EPR13799] (ab184970) at 1/50000 dilution + Saccharomyces cerevisiae extract at 10 µg
Secondary
goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted? -
![Anti-alpha Tubulin antibody [EPR13799] (ab184970)](https://www.abcam.com/ps/products/184/ab184970/Images/ab184970-2-benefits-of-recombinant-antibodies.png)
Anti-alpha Tubulin antibody [EPR13799] (ab184970)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (15)
ab184970 has been referenced in 15 publications.
- Boiero Sanders M et al. Specialization of actin isoforms derived from the loss of key interactions with regulatory factors. EMBO J 41:e107982 (2022). PubMed: 35178724
- Guerra P et al. Systematic In Vivo Characterization of Fluorescent Protein Maturation in Budding Yeast. ACS Synth Biol 11:1129-1141 (2022). PubMed: 35180343
- Wegmann S et al. Linkage reprogramming by tailor-made E3s reveals polyubiquitin chain requirements in DNA-damage bypass. Mol Cell 82:1589-1602.e5 (2022). PubMed: 35263628
- Gramazio S et al. Light-induced fermenter production of derivatives of the sweet protein monellin is maximized in prestationary Saccharomyces cerevisiae cultures. Biotechnol J 17:e2100676 (2022). PubMed: 35481893
- Diessl J et al. Manganese-driven CoQ deficiency. Nat Commun 13:6061 (2022). PubMed: 36229432