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    products/primary-antibodies/amacr-antibody-amacr1864-ab268062.pdf

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Tags & Cell Markers Cell Type Markers Tumor Associated
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Anti-AMACR antibody [AMACR/1864] (ab268062)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody [AMACR/1864] (ab268062)
  • Western blot - Anti-AMACR antibody [AMACR/1864] (ab268062)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody [AMACR/1864] (ab268062)
  • Western blot - Anti-AMACR antibody [AMACR/1864] (ab268062)
  • Western blot - Anti-AMACR antibody [AMACR/1864] (ab268062)
  • Protein Array - Anti-AMACR antibody [AMACR/1864] (ab268062)

Key features and details

  • Mouse monoclonal [AMACR/1864] to AMACR
  • Suitable for: WB, Protein Array, IHC-P
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG1

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Overview

  • Product name

    Anti-AMACR antibody [AMACR/1864]
    See all AMACR primary antibodies
  • Description

    Mouse monoclonal [AMACR/1864] to AMACR
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Protein Array, IHC-Pmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
  • Immunogen

    Recombinant fragment within Human AMACR aa 297-394. The exact sequence is proprietary.
    Database link: Q9UHK6

  • Positive control

    • WB: Human kidney lysate; PC-3 cell lysate; Recombinant AMACR protein. IHC: Human prostate carcinoma and renal cell carcinoma tissues.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from Bioreactor concentrate
  • Clonality

    Monoclonal
  • Clone number

    AMACR/1864
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress

Associated products

  • Alternative Versions

    • Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (ab268183)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab268062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 42 kDa.
Protein Array
Use at an assay dependent concentration.
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 42 kDa.
Protein Array
Use at an assay dependent concentration.
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers.
  • Pathway

    Lipid metabolism; bile acid biosynthesis.
    Lipid metabolism; fatty acid metabolism.
  • Involvement in disease

    Defects in AMACR are the cause of alpha-methylacyl-CoA racemase deficiency (AMACRD) [MIM:614307]. AMACRD results in elevated plasma concentrations of pristanic acid C27-bile-acid intermediates. It can be associated with polyneuropathy, retinitis pigmentosa, epilepsy.
    Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4) [MIM:214950]; also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.
  • Sequence similarities

    Belongs to the CaiB/BaiF CoA-transferase family.
  • Cellular localization

    Peroxisome. Mitochondrion.
  • Target information above from: UniProt accession Q9UHK6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23600 Human
    • Omim: 604489 Human
    • SwissProt: Q9UHK6 Human
    • Unigene: 508343 Human
    • Alternative names

      • 2 arylpropionyl CoA epimerase antibody
      • 2 methylacyl CoA racemase antibody
      • 2-methylacyl-CoA racemase antibody
      • Alpha methylacyl CoA racemase antibody
      • Alpha methylacyl Coenzyme A racemase antibody
      • Alpha methylacyl-CoA racemase deficiency, included antibody
      • Alpha-methylacyl-CoA racemase antibody
      • Amacr antibody
      • AMACR deficiency, included antibody
      • AMACR_HUMAN antibody
      • CBAS4 antibody
      • Da1-8 antibody
      • EC 5.1.99.4 antibody
      • Macr1 antibody
      • Methylacyl CoA racemase alpha antibody
      • RACE antibody
      • RM antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody [AMACR/1864] (ab268062)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody [AMACR/1864] (ab268062)

      Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for AMACR using ab268062 at 2 μg/ml in immunohistochemical analysis. 

    • Western blot - Anti-AMACR antibody [AMACR/1864] (ab268062)
      Western blot - Anti-AMACR antibody [AMACR/1864] (ab268062)
      Anti-AMACR antibody [AMACR/1864] (ab268062) at 2 µg/ml + PC-3 (human prostate adenocarcinoma cell line) cell lysate

      Predicted band size: 42 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody [AMACR/1864] (ab268062)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody [AMACR/1864] (ab268062)

      Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for AMACR using ab268062  at 2 μg/ml in immunohistochemical analysis. 

    • Western blot - Anti-AMACR antibody [AMACR/1864] (ab268062)
      Western blot - Anti-AMACR antibody [AMACR/1864] (ab268062)
      Anti-AMACR antibody [AMACR/1864] (ab268062) at 2 µg/ml + Human kidney tissue lysate

      Predicted band size: 42 kDa

    • Western blot - Anti-AMACR antibody [AMACR/1864] (ab268062)
      Western blot - Anti-AMACR antibody [AMACR/1864] (ab268062)
      All lanes : Anti-AMACR antibody [AMACR/1864] (ab268062) at 2 µg/ml

      Lane 1 : Recombinant AMACR protein
      Lane 2 : Human kidney lysate

      Predicted band size: 42 kDa

    • Protein Array - Anti-AMACR antibody [AMACR/1864] (ab268062)
      Protein Array - Anti-AMACR antibody [AMACR/1864] (ab268062)

      Protein Array containing more than 19,000 full-length human proteins using ab268062. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab268062? Please let us know so that we can cite the reference in this datasheet.

    ab268062 has not yet been referenced specifically in any publications.

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