Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (ab268183)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (ab268183)](https://www.abcam.com/ps/products/268/ab268183/Images/ab268183-3-anti-amacr-antibody-amacr1864-immunohistochemistry-4.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (ab268183)")
Key features and details
- Mouse monoclonal [AMACR/1864] to AMACR - BSA and Azide free
- Suitable for: IHC-P, Protein Array, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-AMACR antibody [AMACR/1864] - BSA and Azide free
See all AMACR primary antibodies -
Description
Mouse monoclonal [AMACR/1864] to AMACR - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, Protein Array, WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment within Human AMACR. The exact sequence is proprietary.
Database link: Q9UHK6 -
Positive control
- WB: Human kidney lysate; PC-3 cell lysate; Recombinant AMACR protein. IHC-P: Human prostate carcinoma and renal cell carcinoma tissues.
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General notes
ab268183 is the carrier-free version of ab268062.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from bioreactor concentrate. -
Clonality
Monoclonal -
Clone number
AMACR/1864 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab268183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| Protein Array |
Use at an assay dependent concentration.
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| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 42 kDa.
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| Notes |
|---|
|
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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Protein Array
Use at an assay dependent concentration. |
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 42 kDa. |
Target
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Function
Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers. -
Pathway
Lipid metabolism; bile acid biosynthesis.
Lipid metabolism; fatty acid metabolism. -
Involvement in disease
Defects in AMACR are the cause of alpha-methylacyl-CoA racemase deficiency (AMACRD) [MIM:614307]. AMACRD results in elevated plasma concentrations of pristanic acid C27-bile-acid intermediates. It can be associated with polyneuropathy, retinitis pigmentosa, epilepsy.
Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4) [MIM:214950]; also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile. -
Sequence similarities
Belongs to the CaiB/BaiF CoA-transferase family. -
Cellular localization
Peroxisome. Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 23600 Human
- Omim: 604489 Human
- SwissProt: Q9UHK6 Human
- Unigene: 508343 Human
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Alternative names
- 2 arylpropionyl CoA epimerase antibody
- 2 methylacyl CoA racemase antibody
- 2-methylacyl-CoA racemase antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (ab268183)
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![Western blot - Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (ab268183)](https://www.abcam.com/ps/products/268/ab268183/Images/ab268183-7-anti-amacr-antibody-amacr1864-western-blot-4.jpg)
Western blot - Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (ab268183) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (ab268183)](https://www.abcam.com/ps/products/268/ab268183/Images/ab268183-4-anti-amacr-antibody-amacr1864-immunohistochemistry-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (ab268183) -
![Western blot - Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (ab268183)](https://www.abcam.com/ps/products/268/ab268183/Images/ab268183-6-anti-amacr-antibody-amacr1864-western-blot-3.jpg)
Western blot - Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (ab268183) -
![Western blot - Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (ab268183)](https://www.abcam.com/ps/products/268/ab268183/Images/ab268183-5-anti-amacr-antibody-amacr1864-western-blot-2.jpg)
Western blot - Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (ab268183) -
![Protein Array - Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (ab268183)](https://www.abcam.com/ps/products/268/ab268183/Images/ab268183-2-anti-amacr-antibody-amacr1864-protein-array-3.jpg)
Protein Array - Anti-AMACR antibody [AMACR/1864] - BSA and Azide free (ab268183)Protein Array containing more than 19,000 full-length human proteins using ab268062.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268062)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab268183 has not yet been referenced specifically in any publications.
