Anti-AMACR antibody [RM349] - N-terminal (ab238312)
Key features and details
- Rabbit monoclonal [RM349] to AMACR - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AMACR antibody [RM349] - N-terminal
See all AMACR primary antibodies -
Description
Rabbit monoclonal [RM349] to AMACR - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human AMACR (N terminal).
Database link: Q9UHK6 -
Positive control
- WB: LNCaP cell lysate. IHC-P: Human kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM349 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab238312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/2000. Predicted molecular weight: 42 kDa.
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IHC-P |
1/500 - 1/1000.
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Notes |
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WB
1/1000 - 1/2000. Predicted molecular weight: 42 kDa. |
IHC-P
1/500 - 1/1000. |
Target
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Function
Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers. -
Pathway
Lipid metabolism; bile acid biosynthesis.
Lipid metabolism; fatty acid metabolism. -
Involvement in disease
Defects in AMACR are the cause of alpha-methylacyl-CoA racemase deficiency (AMACRD) [MIM:614307]. AMACRD results in elevated plasma concentrations of pristanic acid C27-bile-acid intermediates. It can be associated with polyneuropathy, retinitis pigmentosa, epilepsy.
Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4) [MIM:214950]; also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile. -
Sequence similarities
Belongs to the CaiB/BaiF CoA-transferase family. -
Cellular localization
Peroxisome. Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 23600 Human
- Omim: 604489 Human
- SwissProt: Q9UHK6 Human
- Unigene: 508343 Human
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Alternative names
- 2 arylpropionyl CoA epimerase antibody
- 2 methylacyl CoA racemase antibody
- 2-methylacyl-CoA racemase antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab238312 has not yet been referenced specifically in any publications.