Recombinant Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)
![Western blot - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)](https://www.abcam.com/ps/products/137/ab137060/Images/ab137060-412732-anti-amino-terminal-enhancer-of-splitaes-antibody-epr8385-western-blot-human-knockout-lysate.jpg "Western blot - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8385] to Amino-terminal enhancer of split/AES
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Amino-terminal enhancer of split/AES antibody [EPR8385]
See all Amino-terminal enhancer of split/AES primary antibodies -
Description
Rabbit monoclonal [EPR8385] to Amino-terminal enhancer of split/AES -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HCT116, 293T, HeLa, PC3 and Jurkat cell lysates. ICC: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8385 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
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| ICC/IF |
1/250 - 1/500.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa. |
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ICC/IF
1/250 - 1/500. |
Target
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Function
Transcriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development. -
Tissue specificity
Found predominantly in muscle, heart and Placenta. In fetal tissues, abundantly expressed in the heart, lung, kidney, brain and liver. -
Sequence similarities
Belongs to the WD repeat Groucho/TLE family. -
Domain
Lacks the C-terminal WD repeats. -
Post-translational
modificationsUbiquitinated by XIAP/BIRC4. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 166 Human
- Entrez Gene: 14797 Mouse
- Entrez Gene: 29466 Rat
- Omim: 600188 Human
- SwissProt: Q08117 Human
- SwissProt: P63002 Mouse
- SwissProt: P63003 Rat
- Unigene: 515053 Human
see all -
Alternative names
- Aes 1 antibody
- Aes 2 antibody
- aes antibody
see all
Images
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Western blot - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)All lanes : Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060) at 1/1000 dilution
Lane 1 : Wild-type HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
Lane 2 : AES knockout HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 4 : PC3 (Human prostate adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaLanes 1-4: Merged signal (red and green). Green - ab137060 observed at 21 kDa. Red - loading control ab8245 observed at 36 kDa.
ab137060 Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] was shown to specifically react with Amino-terminal enhancer of split/AES in wild-type HCT cells. Loss of signal was observed when knockout cell line ab266886 (knockout cell lysate ab257818) was used. Wild-type and Amino-terminal enhancer of split/AES knockout samples were subjected to SDS-PAGE. ab137060 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)](https://www.abcam.com/ps/products/137/ab137060/Images/ab137060-151568-anti-amino-terminal-enhancer-of-split-antibody-epr8385-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)Immunofluorescent staining of HeLa cells labelling Amino-terminal enhancer of split/AES using ab137060 at 1/250 dilution.
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![Western blot - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)](https://www.abcam.com/ps/products/137/ab137060/Images/ab137060-151569-anti-amino-terminal-enhancer-of-split-antibody-epr8385-western-blot.jpg)
Western blot - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)All lanes : Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : PC3 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 21 kDa -
![Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)](https://www.abcam.com/ps/products/137/ab137060/Images/ab137060-4-benefits-of-recombinant-antibodies.png)
Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab137060 has been referenced in 1 publication.
- Jin Y et al. SIX1 Activation Is Involved in Cell Proliferation, Migration, and Anti-inflammation of Acute Ischemia/Reperfusion Injury in Mice. Front Mol Biosci 8:725319 (2021). PubMed: 34513929