Anti-AMPK alpha 1 antibody (ab3759)
Key features and details
- Rabbit polyclonal to AMPK alpha 1
- Suitable for: IP, WB, IHC-P
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG
Overview
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Product name
Anti-AMPK alpha 1 antibody
See all AMPK alpha 1 primary antibodies -
Description
Rabbit polyclonal to AMPK alpha 1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human
Predicted to work with: Orangutan
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Immunogen
Synthetic peptide within Human AMPK alpha 1 aa 350-450. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. BAA36547.1 (GeneID 5562).
Database link: Q13131 -
Positive control
- WB: Bovine aortic endothelial cell extracts. Rat aortic smooth muscle cell extracts. HepG2 extracts. Human aortic endothelial cell extract. HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IHC-P: Human muscle and pancreas tissue. IP: AMPK alpha 1 IP in HeLa cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at 2-5 µg/mg of lysate.
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| WB | (1) |
1/500 - 1/10000. Predicted molecular weight: 63 kDa.
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| IHC-P |
Use at an assay dependent concentration.
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| Notes |
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IP
Use at 2-5 µg/mg of lysate. |
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WB
1/500 - 1/10000. Predicted molecular weight: 63 kDa. |
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IHC-P
Use at an assay dependent concentration. |
Target
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Function
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 5562 Human
- Entrez Gene: 105787 Mouse
- Entrez Gene: 65248 Rat
- Omim: 602739 Human
- SwissProt: Q13131 Human
- SwissProt: Q5EG47 Mouse
- SwissProt: P54645 Rat
- Unigene: 43322 Human
see all -
Alternative names
- 5 AMP activated protein kinase alpha 1catalytic subunit antibody
- 5 AMP activated protein kinase catalytic alpha 1 chain antibody
- 5' AMP activated protein kinase catalytic subunit alpha 1 antibody
see all
Images
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Western blot - Anti-AMPK alpha 1 antibody (ab3759)All lanes : Anti-AMPK alpha 1 antibody (ab3759) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 63 kDa
Exposure time: 3 secondsLysated prepared using NETN lysis buffer.
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Western blot - Anti-AMPK alpha 1 antibody (ab3759)Samples: Extracts from
1. Bovine aortic endothelial cells
2. Rat aortic smooth muscle cells
3. HepG2 cells
4. Human aortic endothelial cells
Antibody: Affinity purified Rabbit anti-AMPK alpha 1 (ab3759) used at 2ug/ml (1/500).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody (ab3759)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling AMPK alpha 1 with ab3759 at 1/5000 (0.2µg/ml). Detection: DAB. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody (ab3759)IHC image of AMPK alpha 1 staining in Human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3759, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunoprecipitation - Anti-AMPK alpha 1 antibody (ab3759)Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Lane 1 and 2: ab3759 used for IP at 3 µg per reaction, two lots.
Lane 3: Control IgG.
For blotting immunoprecipitated AMPK alpha 1, ab3759 was used at 0.4 µg/ml.
Detection: Chemiluminescence with an exposure time of 3 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (37)
ab3759 has been referenced in 37 publications.
- Gao F et al. SESN1 attenuates the Ox‑LDL‑induced inflammation, apoptosis and endothelial‑mesenchymal transition of human umbilical vein endothelial cells by regulating AMPK/SIRT1/LOX1 signaling. Mol Med Rep 25:N/A (2022). PubMed: 35293601
- Li X et al. GnRH receptor mediates lipid storage in female adipocytes via AMPK pathway. Int J Med Sci 19:1442-1450 (2022). PubMed: 36035370
- Ge J et al. Induction mechanism of cigarette smoke components (CSCs) on dyslipidemia and hepatic steatosis in rats. Lipids Health Dis 21:117 (2022). PubMed: 36348421
- Wang J et al. Altered Liver Metabolism, Mitochondrial Function, Oxidative Status, and Inflammatory Response in Intrauterine Growth Restriction Piglets with Different Growth Patterns before Weaning. Metabolites 12:N/A (2022). PubMed: 36355136
- Yang W et al. Protein expression alteration in hippocampus upon genetic repression of AMPKα isoforms. Hippocampus 31:353-361 (2021). PubMed: 33492732