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    products/primary-antibodies/ampk-alpha-1-phospho-s496-antibody-epr3051-ab92701.pdf

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RecombinantRabMAb

Recombinant Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)

  • Datasheet
  • SDS
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Western blot - Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)
  • Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)
  • Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3051] to AMPK alpha 1 (phospho S496)
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051]
    See all AMPK alpha 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3051] to AMPK alpha 1 (phospho S496)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293T cell lysate; HeLa cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3051
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Amyloid
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

Associated products

  • Alternative Versions

    • Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] - BSA and Azide free (ab247594)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human AMPK alpha 1 protein (ab202156)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab92701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
Notes
Application notes
ICC/IF: 1/100 - 1/250.
IP: 1/10 - 1/100.
WB: 1/1000 - 1/10000. Predicted molecular weight: 64 kDa.

Is unsuitable for Flow Cyt or IHC-P.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

  • Function

    Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 protein kinase domain.
  • Target information above from: UniProt accession Q13131 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5562 Human
    • Omim: 602739 Human
    • SwissProt: Q13131 Human
    • Unigene: 43322 Human
    • Alternative names

      • 5 AMP activated protein kinase alpha 1catalytic subunit antibody
      • 5 AMP activated protein kinase catalytic alpha 1 chain antibody
      • 5' AMP activated protein kinase catalytic subunit alpha 1 antibody
      • 5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody
      • AAPK1 antibody
      • AAPK1_HUMAN antibody
      • ACACA kinase antibody
      • acetyl CoA carboxylase kinase antibody
      • AI194361 antibody
      • AI450832 antibody
      • AL024255 antibody
      • AMP -activate kinase alpha 1 subunit antibody
      • AMP-activate kinase alpha 1 subunit antibody
      • AMP-activated protein kinase, catalytic, alpha -1 antibody
      • AMPK 1 antibody
      • AMPK alpha 1 antibody
      • AMPK alpha 1 chain antibody
      • AMPK antibody
      • AMPK subunit alpha 1 antibody
      • AMPK subunit alpha-1 antibody
      • AMPK1 antibody
      • AMPKa1 antibody
      • AMPKalpha1 antibody
      • C130083N04Rik antibody
      • cb116 antibody
      • EC 2.7.11.1 antibody
      • HMG CoA reductase kinase antibody
      • HMGCR kinase antibody
      • hormone sensitive lipase kinase antibody
      • Hydroxymethylglutaryl CoA reductase kinase antibody
      • im:7154392 antibody
      • kinase AMPK alpha1 antibody
      • MGC33776 antibody
      • MGC57364 antibody
      • OTTHUMP00000161795 antibody
      • OTTHUMP00000161796 antibody
      • PRKAA 1 antibody
      • PRKAA1 antibody
      • Protein kinase AMP activated alpha 1 catalytic subunit antibody
      • SNF1-like protein AMPK antibody
      • SNF1A antibody
      • Tau protein kinase PRKAA1 antibody
      • wu:fa94c10 antibody
      see all

    Images

    • Western blot - Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)
      Western blot - Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)
      All lanes : Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701) at 1/1000 dilution

      Lane 1 : 293T cell lysate untreated
      Lane 2 : 293T cell lysate treated with LP

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 64 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)
      Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)
      Immunofluorescent staining of AMPK alpha 1 in HeLa cells using ab92701 at 1/100 dilution.
    • Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)
      Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (13)

    Publishing research using ab92701? Please let us know so that we can cite the reference in this datasheet.

    ab92701 has been referenced in 13 publications.

    • Zhang H  et al. Rutaecarpine ameliorates lipopolysaccharide-induced BEAS-2B cell injury through inhibition of endoplasmic reticulum stress via activation of the AMPK/SIRT1 signaling pathway. Exp Ther Med 23:373 (2022). PubMed: 35495603
    • Sun Q  et al. Amentoflavone promotes ferroptosis by regulating reactive oxygen species (ROS) /5'AMP-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) to inhibit the malignant progression of endometrial carcinoma cells. Bioengineered 13:13269-13279 (2022). PubMed: 35635082
    • Ma H & Ma Y Dapagliflozin Inhibits Ventricular Remodeling in Heart Failure Rats by Activating Autophagy through AMPK/mTOR Pathway. Comput Math Methods Med 2022:6260202 (2022). PubMed: 36193200
    • Zhang Q  et al. Neuroprotective Effect of miR-483-5p Against Cardiac Arrest-Induced Mitochondrial Dysfunction Mediated Through the TNFSF8/AMPK/JNK Signaling Pathway. Cell Mol Neurobiol N/A:N/A (2022). PubMed: 36266523
    • Yang W  et al. Sinomenine Suppresses Development of Hepatocellular Carcinoma Cells via Inhibiting MARCH1 and AMPK/STAT3 Signaling Pathway. Front Mol Biosci 8:684262 (2021). PubMed: 34179090
    View all Publications for this product

    Customer reviews and Q&As

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