Recombinant Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)
![Western blot - Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)](https://www.abcam.com/ps/products/92/ab92701/Images/ab92701-107896-anti-ampk-alpha-1-phospho-s496-antibody-epr3051-western-blot.jpg "Western blot - Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3051] to AMPK alpha 1 (phospho S496)
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051]
See all AMPK alpha 1 primary antibodies -
Description
Rabbit monoclonal [EPR3051] to AMPK alpha 1 (phospho S496) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293T cell lysate; HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3051 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92701 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| ICC/IF |
| Notes |
|---|
Application notes
ICC/IF: 1/100 - 1/250.
IP: 1/10 - 1/100.
WB: 1/1000 - 1/10000. Predicted molecular weight: 64 kDa.
Is unsuitable for Flow Cyt or IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IP: 1/10 - 1/100.
WB: 1/1000 - 1/10000. Predicted molecular weight: 64 kDa.
Is unsuitable for Flow Cyt or IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 5562 Human
- Omim: 602739 Human
- SwissProt: Q13131 Human
- Unigene: 43322 Human
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Alternative names
- 5 AMP activated protein kinase alpha 1catalytic subunit antibody
- 5 AMP activated protein kinase catalytic alpha 1 chain antibody
- 5' AMP activated protein kinase catalytic subunit alpha 1 antibody
see all
Images
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Western blot - Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)All lanes : Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701) at 1/1000 dilution
Lane 1 : 293T cell lysate untreated
Lane 2 : 293T cell lysate treated with LP
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 64 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)](https://www.abcam.com/ps/products/92/ab92701/Images/ab92701-107895-anti-ampk-alpha-1-phospho-s496-antibody-epr3051-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)Immunofluorescent staining of AMPK alpha 1 in HeLa cells using ab92701 at 1/100 dilution. -
![Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)](https://www.abcam.com/ps/products/92/ab92701/Images/ab92701-3-benefits-of-recombinant-antibodies.png)
Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab92701 has been referenced in 13 publications.
- Zhang H et al. Rutaecarpine ameliorates lipopolysaccharide-induced BEAS-2B cell injury through inhibition of endoplasmic reticulum stress via activation of the AMPK/SIRT1 signaling pathway. Exp Ther Med 23:373 (2022). PubMed: 35495603
- Sun Q et al. Amentoflavone promotes ferroptosis by regulating reactive oxygen species (ROS) /5'AMP-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) to inhibit the malignant progression of endometrial carcinoma cells. Bioengineered 13:13269-13279 (2022). PubMed: 35635082
- Ma H & Ma Y Dapagliflozin Inhibits Ventricular Remodeling in Heart Failure Rats by Activating Autophagy through AMPK/mTOR Pathway. Comput Math Methods Med 2022:6260202 (2022). PubMed: 36193200
- Zhang Q et al. Neuroprotective Effect of miR-483-5p Against Cardiac Arrest-Induced Mitochondrial Dysfunction Mediated Through the TNFSF8/AMPK/JNK Signaling Pathway. Cell Mol Neurobiol N/A:N/A (2022). PubMed: 36266523
- Yang W et al. Sinomenine Suppresses Development of Hepatocellular Carcinoma Cells via Inhibiting MARCH1 and AMPK/STAT3 Signaling Pathway. Front Mol Biosci 8:684262 (2021). PubMed: 34179090