Recombinant Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5683] to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Drosophila melanogaster
Related conjugates and formulations
Overview
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Product name
Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683]
See all AMPK alpha 1+AMPK alpha 2 primary antibodies -
Description
Rabbit monoclonal [EPR5683] to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) -
Host species
Rabbit -
Specificity
This antibody only detects AMPK alpha 1 phosphorylated at Threonine 183 and AMPK alpha 2 phosphorylated Threonine 172.
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Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human, Drosophila melanogaster -
Immunogen
Synthetic peptide within Human AMPK alpha 1 (phospho T183). The exact sequence is proprietary. Also within AMPK alpha 2 (phospho T172).
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Positive control
- WB: HEK-293 and MOLT-4 whole cell lysates; Mouse heart lysate; Rat brain lysate;
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 1.22 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5683 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (4) |
1/1000 - 1/10000. Detects a band of approximately 62,63 kDa (predicted molecular weight: 64 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 62,63 kDa (predicted molecular weight: 64 kDa). |
Target
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Cellular localization
AMPK alpha 2: Cytoplasm. Nucleus. In response to stress, recruited by p53/TP53 to specific promoters. -
Database links
- Entrez Gene: 5562 Human
- Entrez Gene: 5563 Human
- Entrez Gene: 105787 Mouse
- Entrez Gene: 108079 Mouse
- Entrez Gene: 65248 Rat
- Entrez Gene: 78975 Rat
- Omim: 600497 Human
- Omim: 602739 Human
see all
Images
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Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/2000 dilution
Lane 1 : HEK-293 (human embryonic kidney) whole cell lysates
Lane 2 : HEK-293 (human embryonic kidney) whole cell lysates plus AMPK alpha 1 phospho pT183 and AMPK alpha 2 phospho pT172 peptides
Lane 3 : HEK-293 (human embryonic kidney) whole cell lysates plus AMPK alpha 1 and AMPK alpha 2 non-phospho peptides
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), HRP conjugated (ab97051) at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
Exposure time: 15 secondsBlocking and dilution buffer: 5% NFDM/TBST
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Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (purified) + Fruit fly lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 64 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (purified)
Lane 1 : Mouse heart lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 64 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (purified) + Molt-4 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 64 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (purified)
Lane 1 : Untreated HEK-293 cell lysate
Lane 2 : HEK-293 cell lysate treated with Lambda phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 64 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (unpurified)
Lane 1 : 293 cell lysate
Lane 2 : Lysate of 293 cells treated with lambda phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 64 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (125)
ab133448 has been referenced in 125 publications.
- Hou D et al. Uncoupling protein 1 knockout aggravates isoproterenol-induced acute myocardial ischemia via AMPK/mTOR/PPARα pathways in rats. Transgenic Res 31:107-118 (2022). PubMed: 34709566
- Ji R et al. Salidroside alleviates oxidative stress and apoptosis via AMPK/Nrf2 pathway in DHT-induced human granulosa cell line KGN. Arch Biochem Biophys 715:109094 (2022). PubMed: 34813774
- Park CH et al. Canagliflozin protects against cisplatin-induced acute kidney injury by AMPK-mediated autophagy in renal proximal tubular cells. Cell Death Discov 8:12 (2022). PubMed: 35013111
- Chen M et al. Metformin protects lens epithelial cells against senescence in a naturally aged mouse model. Cell Death Discov 8:8 (2022). PubMed: 35013152
- Yang J et al. Pitavastatin activates mitophagy to protect EPC proliferation through a calcium-dependent CAMK1-PINK1 pathway in atherosclerotic mice. Commun Biol 5:124 (2022). PubMed: 35145192