Recombinant Anti-Angiopoietin 1 antibody [EPR2889(N)] (ab183702)
![Western blot - Anti-Angiopoietin 1 antibody [EPR2889(N)] (ab183702)](https://www.abcam.com/ps/products/183/ab183702/Images/ab183702-215717-anti-angiopoietin-1-antibody-epr2889n-western-blot.jpg "Western blot - Anti-Angiopoietin 1 antibody [EPR2889(N)] (ab183702)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2889(N)] to Angiopoietin 1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Angiopoietin 1 antibody [EPR2889(N)]
See all Angiopoietin 1 primary antibodies -
Description
Rabbit monoclonal [EPR2889(N)] to Angiopoietin 1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- U87-MG, ECV-304 and K562 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2889(N) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183702 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa). |
Target
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Function
Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 284 Human
- Omim: 601667 Human
- SwissProt: Q15389 Human
- Unigene: 369675 Human
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Alternative names
- AGP 1 antibody
- AGP1 antibody
- AGPT antibody
see all
Images
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Western blot - Anti-Angiopoietin 1 antibody [EPR2889(N)] (ab183702)All lanes : Anti-Angiopoietin 1 antibody [EPR2889(N)] (ab183702) at 1/5000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : ECV-304 cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 57 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted? -
![Anti-Angiopoietin 1 antibody [EPR2889(N)] (ab183702)](https://www.abcam.com/ps/products/183/ab183702/Images/ab183702-2-benefits-of-recombinant-antibodies.png)
Anti-Angiopoietin 1 antibody [EPR2889(N)] (ab183702)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab183702 has not yet been referenced specifically in any publications.