Recombinant Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)
![Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)](https://www.abcam.com/ps/products/206/ab206420/Images/ab206420-264266-anti-angiopoietin-like-4-antibody-epr19873-western-blot.jpg "Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19873] to Angiopoietin-like 4/ANGPTL4
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873]
See all Angiopoietin-like 4/ANGPTL4 primary antibodies -
Description
Rabbit monoclonal [EPR19873] to Angiopoietin-like 4/ANGPTL4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: A549, SW480, THP-1 and HT-29 whole cell lysates; human kidney and fetal lung lysates. ICC/IF: SW480 and HT-29 cells. Flow Cyt (intra): HT-29 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19873 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab206420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 50, 35 kDa (predicted molecular weight: 45 kDa).
|
|
| ICC/IF |
1/100.
|
|
| Flow Cyt (Intra) |
1/700.
|
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 50, 35 kDa (predicted molecular weight: 45 kDa). |
|
ICC/IF
1/100. |
|
Flow Cyt (Intra)
1/700. |
Target
-
Function
Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorgenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation. -
Tissue specificity
Expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted. Secreted > extracellular space > extracellular matrix. The unprocessed form interacts with the extracellular matrix. This may constitute a dynamic reservoir, a regulatory mechanism of the bioavailability of ANGPTL4. - Information by UniProt
-
Database links
- Entrez Gene: 51129 Human
- Omim: 605910 Human
- SwissProt: Q9BY76 Human
- Unigene: 9613 Human
-
Alternative names
- Angiopoietin like 4 antibody
- Angiopoietin related protein 4 antibody
- Angiopoietin-like protein 4 antibody
see all
Images
-
Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)Lanes 1-3 : Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420) at 1/2000 dilution
Lanes 4-5 : Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : SW480 (human colorectal adenocarcinoma cell line) whole cell lysate
Lane 3 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 4 : Human kidney lysate
Lane 5 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 4-5 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 45 kDa
Observed band size: 35,50 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1-4: 3 minutes; Lane 5: 5 seconds.
Angiopoietin-like 4/ANGPTL4 can be cleaved at 161-164 site and this immunogen recognize full length 50KD and C-Terminal fragment 35KD. (PMID:19270337; PMID:10862772).
-
![Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)](https://www.abcam.com/ps/products/206/ab206420/Images/ab206420-264264-anti-angiopoietin-like-4-antibody-epr19873-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SW480 (human colorectal adenocarcinoma cell line) cells labeling Angiopoietin-like 4/ANGPTL4 with ab206420 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on SW480 cell line. The nuclear counterstain is DAPI (blue).
Alpha-Tubulin is detected with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
-
![Flow Cytometry (Intracellular) - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)](https://www.abcam.com/ps/products/206/ab206420/Images/ab206420-264262-anti-angiopoietin-like-4-antibody-epr19873-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HT-29 (human colorectal adenocarcinoma cell line) cells labeling Angiopoietin-like 4/ANGPTL4 with ab206420 at 1/700 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluorr® 488) at 1/2000 dilution was used as the secondary antibody.
-
![Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)](https://www.abcam.com/ps/products/206/ab206420/Images/ab206420-264265-anti-angiopoietin-like-4-antibody-epr19873-western-blot.jpg)
Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420) at 1/1000 dilution + HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 45 kDa
Observed band size: 35,50 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)](https://www.abcam.com/ps/products/206/ab206420/Images/ab206420-264263-anti-angiopoietin-like-4-antibody-epr19873-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (human colorectal adenocarcinoma cell line) cells labeling Angiopoietin-like 4/ANGPTL4 with ab206420 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HT-29 cell line. The nuclear counterstain is DAPI (blue).
Alpha-Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
-
![Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)](https://www.abcam.com/ps/products/206/ab206420/Images/ab206420-7-benefits-of-recombinant-antibodies.png)
Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab206420 has not yet been referenced specifically in any publications.