Recombinant Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] (ab222739)
![ELISA - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] (ab222739)](https://www.abcam.com/ps/products/222/ab222739/Images/ab222739-6-anti-angiotensin-converting-enzyme-1-antibody-epr22250-204-elisa-mouse-ace-recombinant-protein.jpg "ELISA - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] (ab222739)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22250-204] to Angiotensin Converting Enzyme 1
- Suitable for: WB, IHC-P, IHC-Fr, ELISA
- Reacts with: Mouse, Rat, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204]
See all Angiotensin Converting Enzyme 1 primary antibodies -
Description
Rabbit monoclonal [EPR22250-204] to Angiotensin Converting Enzyme 1 -
Host species
Rabbit -
Specificity
WB is only recommended for mouse. -
Tested applications
Suitable for: WB, IHC-P, IHC-Fr, ELISAmore details -
Species reactivity
Reacts with: Mouse, Rat, Recombinant fragment -
Immunogen
Recombinant fragment within Mouse Angiotensin Converting Enzyme 1 aa 600-1250. The exact sequence is proprietary.
Database link: P09470 -
Positive control
- WB: Mouse liver, brain, heart, kidney and lung lysates; bEnd.3 whole cell lysate. IHC-P: Mouse and rat lung tissues. IHC-Fr: Mouse kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22250-204 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 151 kDa).
|
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| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IHC-Fr |
1/100.
Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
|
| ELISA |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 151 kDa). |
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IHC-Fr
1/100. Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
|
ELISA
Use at an assay dependent concentration. |
Target
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Function
Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. -
Tissue specificity
Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis. -
Involvement in disease
Ischemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
Renal tubular dysgenesis (RTD) [MIM:267430]: Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Note=The disease is caused by mutations affecting the gene represented in this entry.
Microvascular complications of diabetes 3 (MVCD3) [MIM:612624]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
Intracerebral hemorrhage (ICH) [MIM:614519]: A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry. -
Sequence similarities
Belongs to the peptidase M2 family. -
Post-translational
modificationsPhosphorylated by CK2 on Ser-1299; which allows membrane retention. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 11421 Mouse
- Entrez Gene: 24310 Rat
- SwissProt: P09470 Mouse
- SwissProt: P47820 Rat
- Unigene: 754 Mouse
- Unigene: 10149 Rat
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Alternative names
- ACE 1 antibody
- ACE antibody
- ACE T antibody
see all
Images
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ELISA - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] (ab222739)ELISA analysis of Mouse ACE Recombinant Protein recombinant protein at 1000 ng/ml with ab222739. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
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![Western blot - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] (ab222739)](https://www.abcam.com/ps/products/222/ab222739/Images/ab222739-319048-anti-angiotensin-converting-enzyme-1-antibody-epr22250-204-western-blot.jpg)
Western blot - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] (ab222739)All lanes : Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] (ab222739) at 1/1000 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse heart lysate
Lane 4 : Mouse kidney lysate
Lane 5 : bEnd.3 (mouse brain endothelioma cell line) whole cell lysate
Lane 6 : Mouse lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 151 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Exposure time : Lane 1: 81 seconds; Lane 2: 26 seconds; Lane 3: 8 seconds; Lane 4: 3 seconds; Lane 5: 48 seconds; Lane 6: 10 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
Apart from the target band at 180-kDa, weak reactivity bands observed could be due to glycosylation (PMID: 24163131).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] (ab222739)](https://www.abcam.com/ps/products/222/ab222739/Images/ab222739-319050-anti-angiotensin-converting-enzyme-1-antibody-epr22250-204-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] (ab222739)Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Angiotensin Converting Enzyme 1 with ab222739 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on endothelial cells of mouse lung (PMID: 19451697). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Frozen sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] (ab222739)](https://www.abcam.com/ps/products/222/ab222739/Images/ab222739-319049-anti-angiotensin-converting-enzyme-1-antibody-epr22250-204-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] (ab222739)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Angiotensin Converting Enzyme 1 with ab222739 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining on mouse renal tubules (PMID：25664248) is observed.
The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] (ab222739)](https://www.abcam.com/ps/products/222/ab222739/Images/ab222739-319051-anti-angiotensin-converting-enzyme-1-antibody-epr22250-204-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] (ab222739)Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Angiotensin Converting Enzyme 1 with ab222739 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on endothelial cells of rat lung (PMID: 19451697). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] (ab222739)](https://www.abcam.com/ps/products/222/ab222739/Images/ab222739-5-benefits-of-recombinant-antibodies.png)
Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] (ab222739)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab222739 has been referenced in 1 publication.
- Zhou G et al. The regulation effect of WNT-RAS signaling in hypothalamic paraventricular nucleus on renal fibrosis. J Nephrol 33:289-297 (2020). PubMed: 31392659