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    products/primary-antibodies/angiotensin-converting-enzyme-1-antibody-epr22291-247-ab254222.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (1) Submit a question References (6)

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Immunohistochemistry (Frozen sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)
  • Western blot - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)
  • Immunohistochemistry (Frozen sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)
  • Western blot - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)
  • Western blot - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)
  • Indirect ELISA - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)
  • Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22291-247] to Angiotensin Converting Enzyme 1
  • Suitable for: IHC-Fr, WB, IHC-P, Indirect ELISA
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

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Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)
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Recombinant Human ACE2 protein (ab151852)
Protein
Product image
Recombinant Human Angiotensin Converting Enzyme 1 protein (ab158266)

View more associated products

Overview

  • Product name

    Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247]
    See all Angiotensin Converting Enzyme 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR22291-247] to Angiotensin Converting Enzyme 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, WB, IHC-P, Indirect ELISAmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: bEND.3, HUVEC and HAP1 whole cell lysates; human kidney and lung cell lysates; mouse brain, heart, kidney, spleen and lung tissue lysates; rat brain, heart, liver and spleen tisuue lysates. IHC-P: Human kidney and liver tissue; mouse kidney tissue; rat kidney tissue. IHC-Fr: Mouse kidney tissue; rat kidney tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22291-247
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Serum Proteins
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Metalloprotease
    • ACEs
    • Cardiovascular
    • Vasculature
    • Vasoconstriction
    • Other
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cardiovascular ELISA kits
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Associated products

  • Alternative Versions

    • Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] - BSA and Azide free (ab254278)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Immunohistochemistry kits

    • Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
  • Recombinant Protein

    • Recombinant Human Angiotensin Converting Enzyme 1 protein (ab158266)
  • Related Products

    • Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab254222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
1/500.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

WB (1)
1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 150 kDa).
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Indirect ELISA
Use at an assay dependent concentration.
Notes
IHC-Fr
1/500.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

WB
1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 150 kDa).
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Indirect ELISA
Use at an assay dependent concentration.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Target

  • Function

    Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
  • Tissue specificity

    Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis.
  • Involvement in disease

    Ischemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
    Renal tubular dysgenesis (RTD) [MIM:267430]: Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Note=The disease is caused by mutations affecting the gene represented in this entry.
    Microvascular complications of diabetes 3 (MVCD3) [MIM:612624]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
    Intracerebral hemorrhage (ICH) [MIM:614519]: A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
  • Sequence similarities

    Belongs to the peptidase M2 family.
  • Post-translational
    modifications

    Phosphorylated by CK2 on Ser-1299; which allows membrane retention.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession P12821 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1636 Human
    • Entrez Gene: 11421 Mouse
    • Entrez Gene: 24310 Rat
    • Omim: 106180 Human
    • SwissProt: P12821 Human
    • SwissProt: P09470 Mouse
    • SwissProt: P47820 Rat
    • Unigene: 298469 Human
    • Unigene: 754 Mouse
    • Unigene: 10149 Rat
    see all
  • Alternative names

    • ACE 1 antibody
    • ACE antibody
    • ACE T antibody
    • ACE_HUMAN antibody
    • ACE1 antibody
    • Angiotensin converting enzyme somatic isoform antibody
    • Angiotensin converting enzyme testis specific isoform antibody
    • Angiotensin I converting enzyme 1 antibody
    • Angiotensin I converting enzyme antibody
    • Angiotensin I converting enzyme peptidyl dipeptidase A 1 antibody
    • angiotensin I converting enzyme peptidyl-dipeptidase A 1 transcript antibody
    • Angiotensin-converting enzyme antibody
    • Carboxycathepsin antibody
    • CD 143 antibody
    • CD143 antibody
    • CD143 antigen antibody
    • DCP 1 antibody
    • DCP antibody
    • DCP1 antibody
    • Dipeptidyl carboxypeptidase 1 antibody
    • Dipeptidyl carboxypeptidase I antibody
    • Kininase II antibody
    • MGC26566 antibody
    • MVCD3 antibody
    • Peptidase P antibody
    • Peptidyl dipeptidase A antibody
    • soluble form antibody
    • Testicular ECA antibody
    see all

Images

  • Immunohistochemistry (Frozen sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)
    Immunohistochemistry (Frozen sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat kidney tissue labeling Angiotensin Converting Enzyme 1 with ab254222 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Positive staining on proximal tubules of kidney (PMID:10504496) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

     

  • Western blot - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)
    Western blot - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)
    All lanes : Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222) at 1/1000 dilution

    Lane 1 : HUVEC (human umbilical vein endothelial cell line) whole cell lysate at 20 µg
    Lane 2 : Wild-type HAP1 whole cell lysate at 40 µg
    Lane 3 : Angiotensin Converting Enzyme 1 knockout HAP1 whole cell lysate at 40 µg
    Lane 4 : Human kidney cell lysate at 40 µg
    Lane 5 : Human lung cell lysate at 40 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 150 kDa
    Observed band size: 180 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1-3: 3 minutes. Lanes 4-5: 5.5 seconds.

    ab254222 was shown to specifically react with Angiotensin Converting Enzyme 1 in wild-type HAP1 cells as signal was lost in Angiotensin Converting Enzyme 1 knockout cells. Wild-type and Angiotensin Converting Enzyme 1 knockout samples were subjected to SDS-PAGE. ab254222 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

    The molecular weight observed, and the expression profile are consistent with what have been described in the literature (PMID: 25495544, 16203874).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Angiotensin Converting Enzyme 1 with ab254222 at 1/4000 dilution, followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on proximal tubules of mouse kidney (PMID: 2828286; PMID: 175444) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab254222 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Frozen sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)
    Immunohistochemistry (Frozen sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse kidney tissue labeling Angiotensin Converting Enzyme 1 with ab254222 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Positive staining on proximal tubules of kidney (PMID:10504496) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Angiotensin Converting Enzyme 1 with ab254222 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on proximal tubules in mouse kidney (PMID: 2828286; PMID: 175444) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab254222 for 30 mins at room temperature. 
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)
    Western blot - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)
    All lanes : Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222) at 1/1000 dilution

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat heart tissue lysate
    Lane 3 : Rat liver tissue lysate
    Lane 4 : Rat spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 150 kDa
    Observed band size: 180 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lane 1: 48 seconds. Lanes 2 & 3:  3 minutes. Lane 4: 48 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Angiotensin Converting Enzyme 1 with ab254222 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on blood vessels of human liver (PMID: 175444) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab254222 for 30 mins at room temperature. 
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)

    Immunohistochemical analysis of paraffin-embedded human lkidney tissue labeling Angiotensin Converting Enzyme 1 with ab254222 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on proximal tubules of human kidney (PMID: 2828286; PMID: 175444) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab254222 for 30 mins at room temperature. 
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)
    Western blot - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)
    All lanes : Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse spleen tissue lysate
    Lane 5 : Mouse lung tissue lysate
    Lane 6 : bEND.3 (mouse brain endothelioma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 150 kDa
    Observed band size: 180 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1-4: 10 seconds. Lane 5: 5.5 seconds. Lane 6: 48 seconds.

  • Indirect ELISA - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)
    Indirect ELISA - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)
    ELISA analysis of ACE recombinant protein at 1000 ng/mL with ab254222. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
  • Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)
    Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (6)

Publishing research using ab254222? Please let us know so that we can cite the reference in this datasheet.

ab254222 has been referenced in 6 publications.

  • Ma R  et al. l-Borneol Exerted the Neuroprotective Effect by Promoting Angiogenesis Coupled With Neurogenesis via Ang1-VEGF-BDNF Pathway. Front Pharmacol 12:641894 (2021). PubMed: 33746762
  • Liu TH  et al. Network and 16S rRNA Sequencing-Combined Approach Provides Insightal Evidence of Vitamin K2 for Salt-Sensitive Hypertension. Front Nutr 8:639467 (2021). PubMed: 33718421
  • Siljee S  et al. Cancer Stem Cells in Metastatic Head and Neck Cutaneous Squamous Cell Carcinoma Express Components of the Renin-Angiotensin System. Cells 10:N/A (2021). PubMed: 33513805
  • Pruett JE  et al. Impact of SGLT-2 Inhibition on Cardiometabolic Abnormalities in a Rat Model of Polycystic Ovary Syndrome. Int J Mol Sci 22:N/A (2021). PubMed: 33806551
  • Bragina ME  et al. Characterization of the Renin-Angiotensin System in Aged Cavernosal Tissue and its Role in Penile Fibrosis. J Sex Med 17:2129-2140 (2020). PubMed: 32943375
  • Cui C  et al. Vitamin D receptor activation regulates microglia polarization and oxidative stress in spontaneously hypertensive rats and angiotensin II-exposed microglial cells: Role of renin-angiotensin system. Redox Biol 26:101295 (2019). PubMed: 31421410

Customer reviews and Q&As

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Western blot abreview for Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (cardiac muscle)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
20 µg
Specification
cardiac muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Submitted Mar 09 2022

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