Recombinant Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] - BSA and Azide free (ab254278)
![Immunohistochemistry (Frozen sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] - BSA and Azide free (ab254278)](https://www.abcam.com/ps/products/254/ab254278/Images/ab254278-6-254222IHCFr2.jpg "Immunohistochemistry (Frozen sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] - BSA and Azide free (ab254278)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22291-247] to Angiotensin Converting Enzyme 1 - BSA and Azide free
- Suitable for: WB, IHC-P, IHC-Fr, Indirect ELISA
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] - BSA and Azide free
See all Angiotensin Converting Enzyme 1 primary antibodies -
Description
Rabbit monoclonal [EPR22291-247] to Angiotensin Converting Enzyme 1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-Fr, Indirect ELISAmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: bEND.3, HUVEC and HAP1 whole cell lysates; human kidney and lung cell lysates; mouse brain, heart, kidney, spleen and lung tissue lysates; rat brain, heart, liver and spleen tisuue lysates. IHC-P: Human kidney and liver tissue; mouse kidney tissue; rat kidney tissue. IHC-Fr: Mouse kidney tissue; rat kidney tissue.
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General notes
ab254278 is the carrier-free version of ab254222.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22291-247 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab254278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 180 kDa (predicted molecular weight: 150 kDa).
|
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| IHC-Fr |
Use at an assay dependent concentration.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
|
| Indirect ELISA |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 180 kDa (predicted molecular weight: 150 kDa). |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IHC-Fr
Use at an assay dependent concentration. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
|
Indirect ELISA
Use at an assay dependent concentration. |
Target
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Function
Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. -
Tissue specificity
Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis. -
Involvement in disease
Ischemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
Renal tubular dysgenesis (RTD) [MIM:267430]: Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Note=The disease is caused by mutations affecting the gene represented in this entry.
Microvascular complications of diabetes 3 (MVCD3) [MIM:612624]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
Intracerebral hemorrhage (ICH) [MIM:614519]: A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry. -
Sequence similarities
Belongs to the peptidase M2 family. -
Post-translational
modificationsPhosphorylated by CK2 on Ser-1299; which allows membrane retention. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1636 Human
- Entrez Gene: 11421 Mouse
- Entrez Gene: 24310 Rat
- Omim: 106180 Human
- SwissProt: P12821 Human
- SwissProt: P09470 Mouse
- SwissProt: P47820 Rat
- Unigene: 298469 Human
see all -
Alternative names
- ACE 1 antibody
- ACE antibody
- ACE T antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] - BSA and Azide free (ab254278)
Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat kidney tissue labeling Angiotensin Converting Enzyme 1 with ab254222 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Positive staining on proximal tubules of kidney (PMID:10504496) is observed. The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254222).
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![Immunohistochemistry (Frozen sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] - BSA and Azide free (ab254278)](https://www.abcam.com/ps/products/254/ab254278/Images/ab254278-5-254222IHCFr1.jpg)
Immunohistochemistry (Frozen sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] - BSA and Azide free (ab254278)Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse kidney tissue labeling Angiotensin Converting Enzyme 1 with ab254222 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Positive staining on proximal tubules of kidney (PMID:10504496) is observed. The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254222).
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] - BSA and Azide free (ab254278)](https://www.abcam.com/ps/products/254/ab254278/Images/ab254278-4-254222IHC4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] - BSA and Azide free (ab254278)Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Angiotensin Converting Enzyme 1 with ab254222 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on proximal tubules of mouse kidney (PMID: 2828286; PMID: 175444) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab254222 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254222).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] - BSA and Azide free (ab254278)](https://www.abcam.com/ps/products/254/ab254278/Images/ab254278-3-254222IHC3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] - BSA and Azide free (ab254278)Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Angiotensin Converting Enzyme 1 with ab254222 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on proximal tubules in mouse kidney (PMID: 2828286; PMID: 175444) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab254222 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254222).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] - BSA and Azide free (ab254278)](https://www.abcam.com/ps/products/254/ab254278/Images/ab254278-2-254222IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] - BSA and Azide free (ab254278)Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Angiotensin Converting Enzyme 1 with ab254222 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on blood vessels of human liver (PMID: 175444) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab254222 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254222).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] - BSA and Azide free (ab254278)](https://www.abcam.com/ps/products/254/ab254278/Images/ab254278-1-254222IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] - BSA and Azide free (ab254278)Immunohistochemical analysis of paraffin-embedded human lkidney tissue labeling Angiotensin Converting Enzyme 1 with ab254222 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on proximal tubules of human kidney (PMID: 2828286; PMID: 175444) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab254222 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254222).
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![Western blot - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] - BSA and Azide free (ab254278)](https://www.abcam.com/ps/products/254/ab254278/Images/ab254278-344866-anti-angiotensin-converting-emzyme-1-antibody-western-blot-knockout.jpg)
Western blot - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] - BSA and Azide free (ab254278)All lanes : Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (ab254222) at 1/1000 dilution
Lane 1 : HUVEC (human umbilical vein endothelial cell line) whole cell lysate at 20 µg
Lane 2 : Wild-type HAP1 whole cell lysate at 40 µg
Lane 3 : Angiotensin Converting Enzyme 1 knockout HAP1 whole cell lysate at 40 µg
Lane 4 : Human kidney cell lysate at 40 µg
Lane 5 : Human lung cell lysate at 40 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 150 kDaBlocking and dilution buffer: 5% NFDM/TBST.
Exposure times.
Lanes 1-3: 3 minutes. Lanes 4-5: 5.5 seconds.
ab254222 was shown to specifically react with Angiotensin Converting Enzyme 1 in wild-type HAP1 cells as signal was lost in Angiotensin Converting Enzyme 1 knockout cells. Wild-type and Angiotensin Converting Enzyme 1 knockout samples were subjected to SDS-PAGE. ab254222 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
The molecular weight observed, and the expression profile are consistent with what have been described in the literature (PMID: 25495544, 16203874).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254222).
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![Indirect ELISA - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] - BSA and Azide free (ab254278)](https://www.abcam.com/ps/products/254/ab254278/Images/ab254278-8-anti-angiotensin-converting-enzyme-1-antibody-epr22291-247-elisa-ace.jpg)
Indirect ELISA - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] - BSA and Azide free (ab254278) -
![Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] - BSA and Azide free (ab254278)](https://www.abcam.com/ps/products/254/ab254278/Images/ab254278-9-benefits-of-recombinant-antibodies.png)
Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] - BSA and Azide free (ab254278)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab254278 has been referenced in 1 publication.
- Liu R et al. miR-34a targets PAI-1 to regulate urinary microalbumin and renal function in hypertensive mice. Eur J Med Res 25:3 (2020). PubMed: 32178735
