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RecombinantRabMAb

Recombinant Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)

Reviews (1)Q&A (1)References (35)
Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)
  • Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)
  • Indirect ELISA - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)
  • Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)
  • Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)
  • Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3873] to Angiotensin II Type 1 Receptor
  • Suitable for: WB, Indirect ELISA
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Angiotensin II Type 1 Receptor antibody [EPR3873]
    See all Angiotensin II Type 1 Receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR3873] to Angiotensin II Type 1 Receptor

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 53% homology with Erbin/LAP2. Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested applications

    Suitable for: WB, Indirect ELISAmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Angiotensin II Type 1 Receptor aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P30556

  • Positive control

    • WB: C2C12, PC12, HeLa, and HepG2 whole cell lysate (ab7900).

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
Indirect ELISA
Use at an assay dependent concentration.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
Indirect ELISA
Use at an assay dependent concentration.
Application notes
Is unsuitable for Flow Cyt or IHC-P.

Target

  • Function

    Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

  • Tissue specificity

    Liver, lung, adrenal and adrenocortical adenomas.

  • Involvement in disease

    Defects in AGTR1 are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).

  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.

  • Post-translational
    modifications

    C-terminal Ser or Thr residues may be phosphorylated.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AG2S antibody
    • Agtr 1 antibody
    • Agtr1 antibody
    • AGTR1_HUMAN antibody
    • Agtr1a antibody
    • AGTR1B antibody
    • Ang II antibody
    • Angiotensin II receptor type 1 antibody
    • Angiotensin II type-1 receptor antibody
    • Angiotensin receptor 1 antibody
    • Angiotensin receptor 1B antibody
    • AT 1B antibody
    • AT 1r antibody
    • AT1 antibody
    • At1a antibody
    • AT1AR antibody
    • AT1B antibody
    • AT1BR antibody
    • AT1R antibody
    • AT2R1 antibody
    • AT2R1A antibody
    • AT2R1B antibody
    • HAT1R antibody
    • Type 1 angiotensin II receptor antibody
    • Type 1B angiotensin II receptor antibody
    • Type-1 angiotensin II receptor antibody
    see all

Images

  • Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)
    Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)
    Lane 1 : Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/1000 dilution
    Lane 2 : LIF-tag at 1/10000 dilution

    All lanes : LIF tagged Recombinant Human AGTR1 protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 41 kDa
    Observed band size: 62 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: Lane 1: 40 seconds - Lane 2: 80 seconds.

  • Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)
    Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)
    All lanes : Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/2000 dilution (purified)

    Lane 1 : C2C12 cell lysate
    Lane 2 : PC-12 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 41 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Indirect ELISA - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)
    Indirect ELISA - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)

    ELISA analysis of Human Angiotensin II Type 1 Receptor recombinant protein at 250 ng/mL with ab124734. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

  • Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)
    Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)
    Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/2000 dilution (purified) + HT-1080 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 41 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)
    Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)
    All lanes : Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/1000 dilution (unpurified)

    Lane 1 : C2C12 cell lysate
    Lane 2 : PC12 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 41 kDa

  • Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)
    Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)

References (35)

Publishing research using ab124734? Please let us know so that we can cite the reference in this datasheet.

ab124734 has been referenced in 35 publications.

  • Li RJ  et al. Qing Fei Hua Xian Decoction ameliorates bleomycin-induced pulmonary fibrosis by suppressing oxidative stress through balancing ACE-AngII-AT1R/ACE2-Ang-(1-7)-Mas axis. Iran J Basic Med Sci 26:107-113 (2023). PubMed: 36594067
  • Zhu Z  et al. Homocysteine promotes migration of adventitial fibroblasts via angiotensin II type 1 receptor activation to aggravate atherosclerosis. Gen Physiol Biophys 41:535-547 (2022). PubMed: 36454114
  • Kim HY  et al. Oryeongsan (Wulingsan) ameliorates impaired ANP secretion of atria from spontaneously hypertensive rats. Biomed Pharmacother 146:112433 (2022). PubMed: 35062052
  • Liu X  et al. Pre-miRNA Hsa-Let-7a-2: a Novel Intracellular Partner of Angiotensin II Type 2 Receptor Negatively Regulating its Signals. Int J Biol Sci 18:3237-3250 (2022). PubMed: 35637969
  • Nguyen BL  et al. Alterations in renin-angiotensin receptors are not responsible for exercise preconditioning of skeletal muscle fibers. Sports Med Health Sci 3:148-156 (2021). PubMed: 35784524
View all Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Angiotensin II Type 1 Receptor antibody [EPR3873]

 Below Average
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (cardiac muscle)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
20 µg
Specification
cardiac muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 30 2022

Question

I am interested in buying this antibody, but I am puzzled because the WB picture shows only one band and AT-1 receptor is a glycosilated protein. Does this antibody not recognize glycosilated forms of the protein? And also I have noticed that you detected the protein in cellular line extracts, but what about tissues such as kidney or heart? Thanks in advance

Read More
Answer

Thank you for your interest in ab124734, anti AT-1 receptor. The antibody should be capable of detecting glycosylated receptor. The individual bands shown on the datasheet western blot image may be fully glycosylated receptor, assuming this is how the protein is modified in those cell lines, C2C12, HeLa, PC12 and HepG2, and assuming that the unmodified precursor is present in relatively small amounts compared to the mature protein. The banding pattern seems to be consistent with the blot results for most of the other antibodies we have for the receptor.

We do not have data for tissue lysates. Please let me know if you have any other questions or concerns.

Read More

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