Recombinant Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3873] to Angiotensin II Type 1 Receptor
- Suitable for: WB, Indirect ELISA
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Angiotensin II Type 1 Receptor antibody [EPR3873]
See all Angiotensin II Type 1 Receptor primary antibodies -
Description
Rabbit monoclonal [EPR3873] to Angiotensin II Type 1 Receptor -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 53% homology with Erbin/LAP2. Cross-reactivity with this protein has not been confirmed experimentally.
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Tested applications
Suitable for: WB, Indirect ELISAmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Angiotensin II Type 1 Receptor aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P30556 -
Positive control
- WB: C2C12, PC12, HeLa, and HepG2 whole cell lysate (ab7900).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3873 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
|
Indirect ELISA |
Use at an assay dependent concentration.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 41 kDa. |
Indirect ELISA
Use at an assay dependent concentration. |
Target
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Function
Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. -
Tissue specificity
Liver, lung, adrenal and adrenocortical adenomas. -
Involvement in disease
Defects in AGTR1 are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsC-terminal Ser or Thr residues may be phosphorylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 185 Human
- Entrez Gene: 11607 Mouse
- Entrez Gene: 11608 Mouse
- Entrez Gene: 24180 Rat
- Entrez Gene: 81638 Rat
- Omim: 106165 Human
- SwissProt: P30556 Human
- SwissProt: P29754 Mouse
see all -
Alternative names
- AG2S antibody
- Agtr 1 antibody
- Agtr1 antibody
see all
Images
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Lane 1 : Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/1000 dilution
Lane 2 : LIF-tag at 1/10000 dilution
All lanes : LIF tagged Recombinant Human AGTR1 protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: Lane 1: 40 seconds - Lane 2: 80 seconds.
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All lanes : Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/2000 dilution (purified)
Lane 1 : C2C12 cell lysate
Lane 2 : PC-12 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 41 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
ELISA analysis of Human Angiotensin II Type 1 Receptor recombinant protein at 250 ng/mL with ab124734. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
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Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/2000 dilution (purified) + HT-1080 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 41 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
All lanes : Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/1000 dilution (unpurified)
Lane 1 : C2C12 cell lysate
Lane 2 : PC12 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 41 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (35)
ab124734 has been referenced in 35 publications.
- Li RJ et al. Qing Fei Hua Xian Decoction ameliorates bleomycin-induced pulmonary fibrosis by suppressing oxidative stress through balancing ACE-AngII-AT1R/ACE2-Ang-(1-7)-Mas axis. Iran J Basic Med Sci 26:107-113 (2023). PubMed: 36594067
- Zhu Z et al. Homocysteine promotes migration of adventitial fibroblasts via angiotensin II type 1 receptor activation to aggravate atherosclerosis. Gen Physiol Biophys 41:535-547 (2022). PubMed: 36454114
- Kim HY et al. Oryeongsan (Wulingsan) ameliorates impaired ANP secretion of atria from spontaneously hypertensive rats. Biomed Pharmacother 146:112433 (2022). PubMed: 35062052
- Liu X et al. Pre-miRNA Hsa-Let-7a-2: a Novel Intracellular Partner of Angiotensin II Type 2 Receptor Negatively Regulating its Signals. Int J Biol Sci 18:3237-3250 (2022). PubMed: 35637969
- Nguyen BL et al. Alterations in renin-angiotensin receptors are not responsible for exercise preconditioning of skeletal muscle fibers. Sports Med Health Sci 3:148-156 (2021). PubMed: 35784524