Recombinant Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] - BSA and Azide free (ab239995)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3873] to Angiotensin II Type 1 Receptor - BSA and Azide free
- Suitable for: Indirect ELISA, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] - BSA and Azide free
See all Angiotensin II Type 1 Receptor primary antibodies -
Description
Rabbit monoclonal [EPR3873] to Angiotensin II Type 1 Receptor - BSA and Azide free -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 53% homology with Erbin/LAP2. Cross-reactivity with this protein has not been confirmed experimentally.
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Tested applications
Suitable for: Indirect ELISA, WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: C2C12, PC12, HeLa, and HepG2 whole cell lysate (ab7900).
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General notes
ab239995 is the carrier-free version of ab124734.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3873 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab239995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Indirect ELISA |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 43 kDa (predicted molecular weight: 41 kDa).
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Notes |
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Indirect ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 43 kDa (predicted molecular weight: 41 kDa). |
Target
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Function
Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. -
Tissue specificity
Liver, lung, adrenal and adrenocortical adenomas. -
Involvement in disease
Defects in AGTR1 are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsC-terminal Ser or Thr residues may be phosphorylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 185 Human
- Entrez Gene: 11607 Mouse
- Entrez Gene: 11608 Mouse
- Entrez Gene: 24180 Rat
- Entrez Gene: 81638 Rat
- Omim: 106165 Human
- SwissProt: P30556 Human
- SwissProt: P29754 Mouse
see all -
Alternative names
- AG2S antibody
- Agtr 1 antibody
- Agtr1 antibody
see all
Images
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All lanes : Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/2000 dilution (purified)
Lane 1 : C2C12 cell lysate
Lane 2 : PC-12 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 41 kDaThis data was developed using ab124734, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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This data was developed using ab124734, the same antibody clone in a different buffer formulation.
ELISA analysis of Human Angiotensin II Type 1 Receptor recombinant protein at 250 ng/mL with ab124734. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
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Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/2000 dilution (purified) + HT-1080 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 41 kDaThis data was developed using ab124734, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/1000 dilution (unpurified)
Lane 1 : C2C12 cell lysate
Lane 2 : PC12 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 41 kDaThis data was developed using ab124734, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab239995 has not yet been referenced specifically in any publications.