Recombinant Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)
![Western blot - Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)](https://www.abcam.com/ps/products/229/ab229005/Images/ab229005-315952-anti-angiotensinogen-antibody-epr22199-203-western-blot.jpg "Western blot - Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22199-203] to Angiotensinogen
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-Angiotensinogen antibody [EPR22199-203]
See all Angiotensinogen primary antibodies -
Description
Rabbit monoclonal [EPR22199-203] to Angiotensinogen -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse liver, kidney, heart, brain and lung tissue lysate. IHC-P: Mouse kidney and testis tissue. IP: Mouse liver tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22199-203 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 53 kDa.
|
|
| IHC-P | (1) |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| IP |
1/30.
|
| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 53 kDa. |
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IP
1/30. |
Target
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Function
Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-1 (angiotensin 1-10). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (angiotensin 1-8). Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3 (angiotensin 2-8), angiotensin-4 (angiotensin 3-8). Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2.
Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone.
Angiotensin-3 stimulates aldosterone release.
Angiotensin 1-7 is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects (By similarity). Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Genetic variations in AGT are a cause of susceptibility to essential hypertension (EHT) [MIM:145500]. Essential hypertension is a condition in which blood pressure is consistently higher than normal with no identifiable cause.
Defects in AGT are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). -
Sequence similarities
Belongs to the serpin family. -
Post-translational
modificationsBeta-decarboxylation of Asp-34 in angiotensin-2, by mononuclear leukocytes produces alanine. The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 11606 Mouse
- SwissProt: P11859 Mouse
- Unigene: 301626 Mouse
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Alternative names
- Aangiotensinogen (serpin peptidase inhibitor clade A member 8) antibody
- AGT antibody
- AI265500 antibody
see all
Images
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Western blot - Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)All lanes : Anti-Angiotensinogen antibody [EPR22199-203] (ab229005) at 1/1000 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Mouse heart tissue lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Mouse lung tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 53 kDa
Exposure time: 114 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)](https://www.abcam.com/ps/products/229/ab229005/Images/ab229005-315954-anti-angiotensinogen-antibody-epr22199-203-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)Immunohistochemical analysis of paraffin-embedded mouse kidney tissue stained for Angiotensinogen with ab229005 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Cytoplasmic staining in the proximal tubules of mouse kidney (PMID: 7648615) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG ready to use.
Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)](https://www.abcam.com/ps/products/229/ab229005/Images/ab229005-315955-anti-angiotensinogen-antibody-epr22199-203-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)Immunohistochemical analysis of paraffin-embedded mouse testis tissue stained for Angiotensinogen with ab229005 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Cytoplasmic staining in the Leydig cells of mouse testis (PMID: 20361351) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG ready to use.
Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunoprecipitation - Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)](https://www.abcam.com/ps/products/229/ab229005/Images/ab229005-315953-anti-angiotensinogen-antibody-epr22199-203-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)Angiotensinogen was immunoprecipitated from 0.35 mg mouse liver tissue lysates with ab229005 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229005 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
Lane 1: Mouse liver lysate (10 μg) (Input).
Lane 2: ab29005 IP in mouse liver lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229005 in mouse liver lysate (-).Blocking/D ilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds. -
![Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)](https://www.abcam.com/ps/products/229/ab229005/Images/ab229005-5-benefits-of-recombinant-antibodies.png)
Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab229005 has not yet been referenced specifically in any publications.