Recombinant Anti-Angiotensinogen antibody [EPR2930(2)] - BSA and Azide free (ab247620)
![Western blot - Anti-Angiotensinogen antibody [EPR2930(2)] - BSA and Azide free (ab247620)](https://www.abcam.com/ps/products/247/ab247620/Images/ab247620-398023-anti-angiotensinogen-antibody-epr2930-2-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-Angiotensinogen antibody [EPR2930(2)] - BSA and Azide free (ab247620)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2930(2)] to Angiotensinogen - BSA and Azide free
- Suitable for: ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Angiotensinogen antibody [EPR2930(2)] - BSA and Azide free
See all Angiotensinogen primary antibodies -
Description
Rabbit monoclonal [EPR2930(2)] to Angiotensinogen - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247620 is the carrier-free version of ab108294.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2930(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab247620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 53 kDa.
|
| Notes |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 53 kDa. |
Target
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Function
Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-1 (angiotensin 1-10). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (angiotensin 1-8). Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3 (angiotensin 2-8), angiotensin-4 (angiotensin 3-8). Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2.
Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone.
Angiotensin-3 stimulates aldosterone release.
Angiotensin 1-7 is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects (By similarity). Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Genetic variations in AGT are a cause of susceptibility to essential hypertension (EHT) [MIM:145500]. Essential hypertension is a condition in which blood pressure is consistently higher than normal with no identifiable cause.
Defects in AGT are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). -
Sequence similarities
Belongs to the serpin family. -
Post-translational
modificationsBeta-decarboxylation of Asp-34 in angiotensin-2, by mononuclear leukocytes produces alanine. The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 183 Human
- Omim: 106150 Human
- SwissProt: P01019 Human
- Unigene: 19383 Human
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Alternative names
- Aangiotensinogen (serpin peptidase inhibitor clade A member 8) antibody
- AGT antibody
- AI265500 antibody
see all
Images
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Western blot - Anti-Angiotensinogen antibody [EPR2930(2)] - BSA and Azide free (ab247620)All lanes : Anti-Angiotensinogen antibody [EPR2930(2)] (ab108294) at 1/10000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal artery lysate
Lane 3 : Human fetal heart lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Human plasma lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 53 kDaThis data was developed using ab108294, the same antibody clone in a different buffer formulation.
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![Anti-Angiotensinogen antibody [EPR2930(2)] - BSA and Azide free (ab247620)](https://www.abcam.com/ps/products/247/ab247620/Images/ab247620-2-benefits-of-recombinant-antibodies.png)
Anti-Angiotensinogen antibody [EPR2930(2)] - BSA and Azide free (ab247620)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab247620 has not yet been referenced specifically in any publications.
