Recombinant AP Anti-AMPK alpha 1 antibody [Y365] (ab308710)
![AP Rabbit monoclonal [Y365] to AMPK alpha 1 (ab308710)](https://www.abcam.com/ps/products/308/ab308710/Images/ab308710-1-mto-conjugates-benefits-of-recombinant-antibodies.jpg "AP Rabbit monoclonal [Y365] to AMPK alpha 1 (ab308710)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alkaline Phosphatase Rabbit monoclonal [Y365] to AMPK alpha 1
- Suitable for: Target binding affinity, Antibody labelling
- Conjugation: Alkaline Phosphatase
Related conjugates and formulations
Overview
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Product name
AP Anti-AMPK alpha 1 antibody [Y365]
See all AMPK alpha 1 primary antibodies -
Description
Alkaline Phosphatase Rabbit monoclonal [Y365] to AMPK alpha 1 -
Host species
Rabbit -
Conjugation
Alkaline Phosphatase -
Tested applications
Suitable for: Target binding affinity, Antibody labellingmore details -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for Abtrial: learn more here.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.016% Magnesium chloride, 0.001% Zinc chloride -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y365 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- PE Anti-AMPK alpha 1 antibody [Y365] (ab303053)
- APC Anti-AMPK alpha 1 antibody [Y365] (ab303054)
- HRP Anti-AMPK alpha 1 antibody [Y365] (ab303055)
- Alexa Fluor® 488 Anti-AMPK alpha 1 antibody [Y365] (ab309679)
- Alexa Fluor® 647 Anti-AMPK alpha 1 antibody [Y365] (ab310041)
- Alexa Fluor® 594 Anti-AMPK alpha 1 antibody [Y365] (ab310429)
- Alexa Fluor® 555 Anti-AMPK alpha 1 antibody [Y365] (ab311955)
- Alexa Fluor® 568 Anti-AMPK alpha 1 antibody [Y365] (ab312428)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab308710 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Target binding affinity |
Use at an assay dependent concentration.
|
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| Antibody labelling |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Target binding affinity
Use at an assay dependent concentration. |
|
Antibody labelling
Use at an assay dependent concentration. |
Target
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Function
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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Alternative names
- 5 AMP activated protein kinase alpha 1catalytic subunit antibody
- 5 AMP activated protein kinase catalytic alpha 1 chain antibody
- 5' AMP activated protein kinase catalytic subunit alpha 1 antibody
see all
Images
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AP Rabbit monoclonal [Y365] to AMPK alpha 1 (ab308710)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab308710 has not yet been referenced specifically in any publications.