Recombinant AP Anti-Dnmt3a antibody [EPR18455] (ab308817)
![AP Rabbit monoclonal [EPR18455] to Dnmt3a (ab308817)](https://www.abcam.com/ps/products/308/ab308817/Images/ab308817-1-mto-conjugates-benefits-of-recombinant-antibodies.jpg "AP Rabbit monoclonal [EPR18455] to Dnmt3a (ab308817)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alkaline Phosphatase Rabbit monoclonal [EPR18455] to Dnmt3a
- Suitable for: Target binding affinity, Antibody labelling
- Conjugation: Alkaline Phosphatase
Related conjugates and formulations
Overview
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Product name
AP Anti-Dnmt3a antibody [EPR18455]
See all Dnmt3a primary antibodies -
Description
Alkaline Phosphatase Rabbit monoclonal [EPR18455] to Dnmt3a -
Host species
Rabbit -
Conjugation
Alkaline Phosphatase -
Tested applications
Suitable for: Target binding affinity, Antibody labellingmore details -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for Abtrial: learn more here.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.016% Magnesium chloride, 0.001% Zinc chloride -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18455 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- PE Anti-Dnmt3a antibody [EPR18455] (ab305716)
- APC Anti-Dnmt3a antibody [EPR18455] (ab305717)
- HRP Anti-Dnmt3a antibody [EPR18455] (ab305718)
- Alexa Fluor® 488 Anti-Dnmt3a antibody [EPR18455] (ab309762)
- Alexa Fluor® 647 Anti-Dnmt3a antibody [EPR18455] (ab310130)
- Alexa Fluor® 594 Anti-Dnmt3a antibody [EPR18455] (ab310548)
- Alexa Fluor® 555 Anti-Dnmt3a antibody [EPR18455] (ab312078)
- Alexa Fluor® 568 Anti-Dnmt3a antibody [EPR18455] (ab312555)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab308817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Target binding affinity |
Use at an assay dependent concentration.
|
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| Antibody labelling |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Target binding affinity
Use at an assay dependent concentration. |
|
Antibody labelling
Use at an assay dependent concentration. |
Target
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Function
Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity. -
Tissue specificity
Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung. -
Sequence similarities
Belongs to the C5-methyltransferase family.
Contains 1 ADD domain.
Contains 1 GATA-type zinc finger.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain. -
Domain
The PWWP domain is essential for targeting to pericentric heterochromatin. -
Post-translational
modificationsSumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription. -
Cellular localization
Nucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin. - Information by UniProt
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Alternative names
- DNA (cytosine 5) methyltransferase 3 alpha antibody
- DNA (cytosine 5) methyltransferase 3A antibody
- DNA (cytosine-5)-methyltransferase 3A antibody
see all
Images
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AP Rabbit monoclonal [EPR18455] to Dnmt3a (ab308817)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab308817 has not yet been referenced specifically in any publications.