Recombinant APC Anti-alpha smooth muscle Actin antibody [EPR5368] (ab225143)
![Flow Cytometry (Intracellular) - APC Anti-alpha smooth muscle Actin antibody [EPR5368] (ab225143)](https://www.abcam.com/ps/products/225/ab225143/Images/ab225143-341212-ab225143FLOW1.jpg "Flow Cytometry (Intracellular) - APC Anti-alpha smooth muscle Actin antibody [EPR5368] (ab225143)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- APC Rabbit monoclonal [EPR5368] to alpha smooth muscle Actin
- Suitable for: Flow Cyt (Intra)
- Reacts with: Rat
- Conjugation: APC. Ex: 645nm, Em: 660nm
Related conjugates and formulations
Overview
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Product name
APC Anti-alpha smooth muscle Actin antibody [EPR5368]
See all alpha smooth muscle Actin primary antibodies -
Description
APC Rabbit monoclonal [EPR5368] to alpha smooth muscle Actin -
Host species
Rabbit -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt (intra): SV40LT-SMC cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark. -
Dissociation constant (KD)
KD = 2.20 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5368 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [EPR5368] (ab202295)
- Alexa Fluor® 647 Anti-alpha smooth muscle Actin antibody [EPR5368] (ab202296)
- Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368] (ab202509)
- Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [EPR5368] (ab202510)
- PE Anti-alpha smooth muscle Actin antibody [EPR5368] (ab208844)
- Anti-alpha smooth muscle Actin antibody [EPR5368] - BSA and Azide free (ab220795)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab225143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/5000.
The cellular localisation of this product has been verified in ICC/IF. |
| Notes |
|---|
|
Flow Cyt (Intra)
1/5000. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. -
Sequence similarities
Belongs to the actin family. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 59 Human
- Entrez Gene: 11475 Mouse
- Entrez Gene: 81633 Rat
- Omim: 102620 Human
- SwissProt: P62736 Human
- SwissProt: P62737 Mouse
- SwissProt: P62738 Rat
- Unigene: 500483 Human
see all -
Alternative names
- a actin antibody
- AAT6 antibody
- ACTA_HUMAN antibody
see all
Images
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Flow Cytometry (Intracellular) - APC Anti-alpha smooth muscle Actin antibody [EPR5368] (ab225143)
Overlay histogram showing SV40LT-SMC cells stained with ab225143 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225143, 1/5000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin (ab232814) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.
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![APC Anti-alpha smooth muscle Actin antibody [EPR5368] (ab225143)](https://www.abcam.com/ps/products/225/ab225143/Images/ab225143-3-benefits-of-recombinant-antibodies.png)
APC Anti-alpha smooth muscle Actin antibody [EPR5368] (ab225143)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab225143 has not yet been referenced specifically in any publications.
