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    products/primary-antibodies/apc-cd90--thy1-antibody-5e10-ab139364.pdf

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Immunology Adaptive Immunity T Cells CD
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APC Anti-CD90 / Thy1 antibody [5E10] (ab139364)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (9)

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Flow Cytometry - APC Anti-CD90 / Thy1 antibody [5E10] (ab139364)

    Key features and details

    • APC Mouse monoclonal [5E10] to CD90 / Thy1
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: APC. Ex: 645nm, Em: 660nm
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    APC/Cy7® Biotin FITC PE PE/Cy5® PerCP/Cy5.5® Unconjugated

    You may also be interested in

    Protein
    Recombinant Human CD90 / Thy1 protein (Fc Chimera) (ab157072)
    Conjugation
    Product image
    APC Conjugation Kit - Lightning-Link® (ab201807)
    Knockout
    Product image
    Human THY1 (CD90) knockout HeLa cell line (ab255440)

    View more associated products

    Overview

    • Product name

      APC Anti-CD90 / Thy1 antibody [5E10]
      See all CD90 / Thy1 primary antibodies
    • Description

      APC Mouse monoclonal [5E10] to CD90 / Thy1
    • Host species

      Mouse
    • Conjugation

      APC. Ex: 645nm, Em: 660nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Tissue, cells or virus corresponding to Human CD90/ Thy1. Human erythroleukemia cell line cell preparation.

    • Positive control

      • Flow Cyt: Jurkat cells.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.4
      Preservative: 0.1% Sodium azide
      Constituents: 99% PBS, 0.2% BSA

      BSA is high-grade protease free
    • Concentration information loading...
    • Purity

      Size exclusion
    • Clonality

      Monoclonal
    • Clone number

      5E10
    • Isotype

      IgG1
    • Research areas

      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Synapse marker
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Monocytic Lineage

    Associated products

    • Alternative Versions

      • Anti-CD90 / Thy1 antibody [5E10] (ab123511)
      • FITC Anti-CD90 / Thy1 antibody [5E10] (ab124527)
      • PerCP/Cy5.5® Anti-CD90 / Thy1 antibody [5E10] (ab134360)
      • Biotin Anti-CD90 / Thy1 antibody [5E10] (ab134361)
      • PE/Cy5® Anti-CD90 / Thy1 antibody [5E10] (ab95698)
      • PE Anti-CD90 / Thy1 antibody [5E10] (ab95700)
    • Isotype control

      • APC Mouse IgG1 [1F8] - Isotype Control (ab91358)
    • Recombinant Protein

      • Recombinant Human CD90 / Thy1 protein (Fc Chimera) (ab157072)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab139364 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt (1)
    Use at an assay dependent concentration.

    10 μl reagent / 100 μl of whole blood or 106 cells in a suspension.

    Notes
    Flow Cyt
    Use at an assay dependent concentration.

    10 μl reagent / 100 μl of whole blood or 106 cells in a suspension.

    Target

    • Function

      May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
    • Sequence similarities

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P04216 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7070 Human
      • Omim: 188230 Human
      • SwissProt: P04216 Human
      • Unigene: 644697 Human
      • Alternative names

        • CD7 antibody
        • CD90 antibody
        • CD90 antigen antibody
        • CDw90 antibody
        • FLJ33325 antibody
        • MGC128895 antibody
        • T25 antibody
        • Theta antigen antibody
        • Thy 1 antibody
        • Thy 1 cell surface antigen antibody
        • Thy 1 membrane glycoprotein antibody
        • Thy 1 T cell antigen antibody
        • Thy 1.2 antibody
        • Thy-1 antigen antibody
        • Thy-1 membrane glycoprotein antibody
        • Thy1 antibody
        • Thy1 antigen antibody
        • Thy1 T cell antigen antibody
        • Thy1.1 antibody
        • Thy1.2 antibody
        • THY1_HUMAN antibody
        • Thymus cell antigen 1, theta antibody
        see all

      Images

      • Flow Cytometry - APC Anti-CD90 / Thy1 antibody [5E10] (ab139364)
        Flow Cytometry - APC Anti-CD90 / Thy1 antibody [5E10] (ab139364)
        Flow cytometry analysis (surface staining) of Jurkat cells (red) and SP2 cells (blue) labeling CD90 with ab139364.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (9)

      Publishing research using ab139364? Please let us know so that we can cite the reference in this datasheet.

      ab139364 has been referenced in 9 publications.

      • Zhu S  et al. Bidirectional ephrinB2-EphB4 signaling regulates the osteogenic differentiation of canine periodontal ligament stem cells. Int J Mol Med 45:897-909 (2020). PubMed: 31985015
      • Yan M  et al. Compare features of human dental pulp cells cultured from pulp tissues with and without cryopreservation. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repu N/A:N/A (2020). PubMed: 33325456
      • Tugnoli B  et al. Butyric acid induces spontaneous adipocytic differentiation of porcine bone marrow-derived mesenchymal stem cells. In Vitro Cell Dev Biol Anim 55:17-24 (2019). PubMed: 30456456
      • Dothel G  et al. Ex vivo effect of vascular wall stromal cells secretome on enteric ganglia. World J Gastroenterol 25:4892-4903 (2019). PubMed: 31543681
      • Burmeister DM  et al. Delivery of Allogeneic Adipose Stem Cells in Polyethylene Glycol-Fibrin Hydrogels as an Adjunct to Meshed Autografts After Sharp Debridement of Deep Partial Thickness Burns. Stem Cells Transl Med 7:360-372 (2018). ICC/IF ; Pig . PubMed: 29457376
      • McDaniel JS  et al. Quantitative Assessment of Optimal Bone Marrow Site for the Isolation of Porcine Mesenchymal Stem Cells. Stem Cells Int 2017:1836960 (2017). Flow Cyt ; Pig . PubMed: 28539939
      • Zaniboni A  et al. In vitro differentiation of porcine aortic vascular precursor cells to endothelial and vascular smooth muscle cells. Am J Physiol Cell Physiol 309:C320-31 (2015). Flow Cyt ; Human . PubMed: 26135800
      • Baulier E  et al. Amniotic fluid-derived mesenchymal stem cells prevent fibrosis and preserve renal function in a preclinical porcine model of kidney transplantation. Stem Cells Transl Med 3:809-20 (2014). PubMed: 24797827
      • Zaniboni A  et al. Cells derived from porcine aorta tunica media show mesenchymal stromal-like cell properties in in vitro culture. Am J Physiol Cell Physiol 306:C322-33 (2014). Flow Cyt ; Pig . PubMed: 24304832

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Flow Cytometry abreview for Anti-CD90 / Thy1 antibody [5E10] (APC)

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Bos taurus Cell (Umbilical Cord Mesenchymal Stem Cells)
      Permeabilization
      No
      Gating Strategy
      Sample had one population of cells, gating includes 96.5% cells.
      Specification
      Umbilical Cord Mesenchymal Stem Cells
      Preparation
      Cell harvesting/tissue preparation method: Harvesting of cells by trypsination for 3 minutes
      Sample buffer: 10% chicken serum in PBS
      Fixation
      No fixation
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Oct 01 2020

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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