Recombinant APC Anti-HDAC1 antibody [EPR460(2)] (ab305394)
![APC Anti-HDAC1 antibody [EPR460(2)] (ab305394)](https://www.abcam.com/ps/products/305/ab305394/Images/ab305394-1-mto-conjugates-benefits-of-recombinant-antibodies.jpg "APC Anti-HDAC1 antibody [EPR460(2)] (ab305394)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- APC Rabbit monoclonal [EPR460(2)] to HDAC1
- Suitable for: Antibody labelling, Target binding affinity
- Conjugation: APC. Ex: 645nm, Em: 660nm
Related conjugates and formulations
Overview
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Product name
APC Anti-HDAC1 antibody [EPR460(2)]
See all HDAC1 primary antibodies -
Description
APC Rabbit monoclonal [EPR460(2)] to HDAC1 -
Host species
Rabbit -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Antibody labelling, Target binding affinitymore details -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for Abtrial: learn more here.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR460(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab305394 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Antibody labelling |
Use at an assay dependent concentration.
|
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| Target binding affinity |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Antibody labelling
Use at an assay dependent concentration. |
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Target binding affinity
Use at an assay dependent concentration. |
Target
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Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. -
Tissue specificity
Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily. -
Post-translational
modificationsSumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
Ubiquitinated by CHFR, leading to its degradation by the proteasome. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- DKFZp686H12203 antibody
- GON 10 antibody
- HD1 antibody
see all
Images
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APC Anti-HDAC1 antibody [EPR460(2)] (ab305394)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab305394 has not yet been referenced specifically in any publications.