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    products/primary-antibodies/apc-myc-tag-antibody-9e10-ab223896.pdf

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Tags & Cell Markers Epitope Tags Myc Tag
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APC Anti-Myc tag antibody [9E10] (ab223896)

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Flow Cytometry (Intracellular) - APC Anti-Myc tag antibody [9E10] (ab223896)

    Key features and details

    • APC Mouse monoclonal [9E10] to Myc tag
    • Suitable for: Flow Cyt (Intra)
    • Reacts with: Recombinant fragment
    • Conjugation: APC. Ex: 645nm, Em: 660nm
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 Biotin Biotin DyLight® 488 DyLight® 550 DyLight® 650 FITC HRP PE SureLight® APC Unconjugated

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    Overview

    • Product name

      APC Anti-Myc tag antibody [9E10]
      See all Myc tag primary antibodies
    • Description

      APC Mouse monoclonal [9E10] to Myc tag
    • Host species

      Mouse
    • Conjugation

      APC. Ex: 645nm, Em: 660nm
    • Specificity

      This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc. A publication by Baker AM et al. 2016 (PMID: 26826706 DOI: 10.1111/his.12939) shows the IHC staining generated by the 9E10 clone does not correlate with c-myc mRNA expression.
    • Tested applications

      Suitable for: Flow Cyt (Intra)more details
    • Species reactivity

      Reacts with: Recombinant fragment
    • Immunogen

      Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    • Epitope

      aa 410-419 of human Myc.
    • Positive control

      • Flow Cyt (Intra): 293T cells transfected with TMEM119 with myc tag
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Store In the Dark.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 1% BSA
    • Concentration information loading...
    • Purity

      IgG fraction
    • Clonality

      Monoclonal
    • Clone number

      9E10
    • Myeloma

      Sp2/0
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Tags & Cell Markers
      • Epitope Tags
      • Myc Tag

    Associated products

    • Alternative Versions

      • DyLight® 650 Anti-Myc tag antibody [9E10] (ab117487)
      • DyLight® 488 Anti-Myc tag antibody [9E10] (ab117499)
      • DyLight® 550 Anti-Myc tag antibody [9E10] (ab117511)
      • FITC Anti-Myc tag antibody [9E10] (ab117599)
      • Biotin Anti-Myc tag antibody [9E10] - C-terminal (ab197139)
      • Alexa Fluor® 488 Anti-Myc tag antibody [9E10] (ab202008)
      • Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)
      • Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)
      • Anti-Myc tag antibody [9E10] - BSA and Azide free (ab264603)
      • Anti-Myc tag antibody [9E10] (ab32)
      • HRP Anti-Myc tag antibody [9E10] (ab62928)
      • PE Anti-Myc tag antibody [9E10] (ab72468)
      • SureLight® APC Anti-Myc tag antibody [9E10] (ab72580)
      • Biotin Anti-Myc tag antibody [9E10] (ab81658)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab223896 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt (Intra)
    1/500.

    The cellular localisation of this product has been verified in ICC/IF.

    Notes
    Flow Cyt (Intra)
    1/500.

    The cellular localisation of this product has been verified in ICC/IF.

    Target

    • Relevance

      Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
    • Cellular localization

      Nuclear
    • Alternative names

      • c-myc tag antibody
      • Myc Epitope Tag antibody

    Images

    • Flow Cytometry (Intracellular) - APC Anti-Myc tag antibody [9E10] (ab223896)
      Flow Cytometry (Intracellular) - APC Anti-Myc tag antibody [9E10] (ab223896)

      Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-tagged TMEM119 expression vector labelling Myc-tag with ab223896 at 1/500 dilution (right) compared with Mouse IgG1 (monoclonal) Allophycocyanin (left). Cells were surface-stained with TMEM119 antibody (ab210405), then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Allophycocyanin conjugated Myc-tag antibody (ab223896) and Alexa Fluor® 488 conjugated anti-Rabbit secondary antibody (for TMEM119). Only TMEM119 (+) population showed Myc-tag positive staining.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab223896? Please let us know so that we can cite the reference in this datasheet.

    ab223896 has not yet been referenced specifically in any publications.

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