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RecombinantRabMAb

Recombinant APC Anti-PD-L1 antibody [28-8] (ab206967)

  • Datasheet
  • Certificate of Compliance
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Flow Cytometry - APC Anti-PD-L1 antibody [28-8] (ab206967)
  • APC Anti-PD-L1 antibody [28-8] (ab206967)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • APC Rabbit monoclonal [28-8] to PD-L1
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: APC. Ex: 645nm, Em: 660nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free FITC HRP PE Unconjugated

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Recombinant human PD-L1 protein (Active) (ab167713)
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Overview

  • Product name

    APC Anti-PD-L1 antibody [28-8]
    See all PD-L1 primary antibodies
  • Description

    APC Rabbit monoclonal [28-8] to PD-L1
  • Host species

    Rabbit
  • Conjugation

    APC. Ex: 645nm, Em: 660nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human PD-L1 (extracellular). The exact sequence is proprietary.
    Database link: Q9NZQ7

  • Positive control

    • Flow Cyt: CHO-PDL1 cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    28-8
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Non-lineage
    • Immunology
    • Immune System Diseases
    • Autoimmune
    • Cancer
    • Tumor immunology
    • Tumor-associated antigens
    • Cancer
    • Tumor biomarkers
    • Tumor antigens

Associated products

  • Alternative Versions

    • Anti-PD-L1 antibody [28-8] (ab205921)
    • Anti-PD-L1 antibody [28-8] - Low endotoxin, Azide free (ab209889)
    • Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (ab209959)
    • Alexa Fluor® 647 Anti-PD-L1 antibody [28-8] - Extracellular domain (ab209960)
    • HRP Anti-PD-L1 antibody [28-8] (ab209961)
    • PE Anti-PD-L1 antibody [28-8] (ab209962)
    • Alexa Fluor® 555 Anti-PD-L1 antibody [28-8] (ab213358)
    • Alexa Fluor® 568 Anti-PD-L1 antibody [28-8] (ab213359)
    • Alexa Fluor® 594 Anti-PD-L1 antibody [28-8] (ab213360)
    • FITC Anti-PD-L1 antibody [28-8] (ab224027)
    • Anti-PD-L1 antibody [28-8] - BSA and Azide free (ab228413)
  • Isotype control

    • APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)
  • Recombinant Protein

    • Recombinant human PD-L1 protein (Active) (ab167713)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab206967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/500.

The cellular localisation of this product has been verified in ICC/IF.

Notes
Flow Cyt
1/500.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Function

    Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
  • Tissue specificity

    Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane and Endomembrane system.
  • Target information above from: UniProt accession Q9NZQ7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 29126 Human
    • Omim: 605402 Human
    • SwissProt: Q9NZQ7 Human
    • Unigene: 521989 Human
    • Alternative names

      • B7 H antibody
      • B7 H1 antibody
      • B7 homolog 1 antibody
      • B7-H1 antibody
      • B7H antibody
      • B7H1 antibody
      • CD 274 antibody
      • CD-274 antibody
      • CD274 antibody
      • CD274 antigen antibody
      • CD274 molecule antibody
      • MGC142294 antibody
      • MGC142296 antibody
      • OTTHUMP00000021029 antibody
      • PD L1 antibody
      • PD-L1 antibody
      • PD1L1_HUMAN antibody
      • PDCD1 ligand 1 antibody
      • PDCD1L1 antibody
      • PDCD1LG1 antibody
      • PDL 1 antibody
      • PDL1 antibody
      • Programmed cell death 1 ligand 1 antibody
      • Programmed death ligand 1 antibody
      • RGD1566211 antibody
      see all

    Images

    • Flow Cytometry - APC Anti-PD-L1 antibody [28-8] (ab206967)
      Flow Cytometry - APC Anti-PD-L1 antibody [28-8] (ab206967)

      Overlay histogram showing CHO-S (blue line) and CHO-PD-L1 transfected (red line) cells stained with ab206967.

      The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab206967, 1/500 dilution) for 30 min at 22ºC.

      Acquisition of >5,000 events were collected using a 17 mW red Helium-Neon laser (633nm) and 660/20 bandpass filter.

    • APC Anti-PD-L1 antibody [28-8] (ab206967)
      APC Anti-PD-L1 antibody [28-8] (ab206967)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab206967? Please let us know so that we can cite the reference in this datasheet.

    ab206967 has not yet been referenced specifically in any publications.

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