Recombinant APC Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab225341)
![Flow Cytometry (Intracellular) - APC Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab225341)](https://www.abcam.com/ps/products/225/ab225341/Images/ab225341-307463-anti-tomm20-antibody-epr15581-54-mitochondrial-marker-allophycocyanin-flow-cytometry.jpg "Flow Cytometry (Intracellular) - APC Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab225341)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- APC Rabbit monoclonal [EPR15581-54] to TOMM20 - Mitochondrial Marker
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
Related conjugates and formulations
Overview
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Product name
APC Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker
See all TOMM20 primary antibodies -
Description
APC Rabbit monoclonal [EPR15581-54] to TOMM20 - Mitochondrial Marker -
Host species
Rabbit -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment within Human TOMM20 aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q15388 -
Positive control
- Flow Cyt (intra): HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15581-54 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
- Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab209606)
- HRP Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab209951)
- Alexa Fluor® 405 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab210047)
- Alexa Fluor® 594 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab210665)
- Alexa Fluor® 568 Anti-TOMM20 antibody [EPR15581-54] (ab210841)
- Alexa Fluor® 555 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab221292)
- PE Anti-TOMM20 antibody [EPR15581-54] (ab225342)
- Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab225341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/5000.
The cellular localisation of this product has been verified in ICC/IF. |
| Notes |
|---|
|
Flow Cyt (Intra)
1/5000. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Function
Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. -
Sequence similarities
Belongs to the Tom20 family. -
Cellular localization
Mitochondrion outer membrane. - Information by UniProt
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Database links
- Entrez Gene: 9804 Human
- Entrez Gene: 67952 Mouse
- Entrez Gene: 266601 Rat
- Omim: 601848 Human
- SwissProt: Q15388 Human
- SwissProt: Q9DCC8 Mouse
- SwissProt: Q62760 Rat
- Unigene: 533192 Human
see all -
Alternative names
- KIAA0016 antibody
- MAS20 antibody
- MGC117367 antibody
see all
Images
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Flow Cytometry (Intracellular) - APC Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab225341)
Overlay histogram showing HeLa cells stained with ab225341 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225341, 1/5000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.
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![APC Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab225341)](https://www.abcam.com/ps/products/225/ab225341/Images/ab225341-3-benefits-of-recombinant-antibodies.png)
APC Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab225341)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab225341 has not yet been referenced specifically in any publications.
