Recombinant APC Anti-WSTF antibody [EP1704Y] (ab306266)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- APC Rabbit monoclonal [EP1704Y] to WSTF
- Suitable for: Target binding affinity, Antibody labelling
- Conjugation: APC. Ex: 645nm, Em: 660nm
Related conjugates and formulations
Overview
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Product name
APC Anti-WSTF antibody [EP1704Y]
See all WSTF primary antibodies -
Description
APC Rabbit monoclonal [EP1704Y] to WSTF -
Host species
Rabbit -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Target binding affinity, Antibody labellingmore details -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for Abtrial: learn more here.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1704Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-WSTF antibody [EP1704Y] - BSA and Azide free (ab235388)
- AP Anti-WSTF antibody [EP1704Y] (ab308886)
- Alexa Fluor® 488 Anti-WSTF antibody [EP1704Y] (ab309805)
- Alexa Fluor® 647 Anti-WSTF antibody [EP1704Y] (ab310174)
- Alexa Fluor® 594 Anti-WSTF antibody [EP1704Y] (ab310612)
- Alexa Fluor® 555 Anti-WSTF antibody [EP1704Y] (ab312140)
- Alexa Fluor® 568 Anti-WSTF antibody [EP1704Y] (ab312626)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab306266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Target binding affinity |
Use at an assay dependent concentration.
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Antibody labelling |
Use at an assay dependent concentration.
|
Notes |
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Target binding affinity
Use at an assay dependent concentration. |
Antibody labelling
Use at an assay dependent concentration. |
Target
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Function
Atypical tyrosine-protein kinase that plays a central role in chromatin remodeling and acts as a transcription regulator. Involved in DNA damage response by phosphorylating 'Tyr-142' of histone H2AX (H2AXY142ph). H2AXY142ph plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. In the complex, it mediates the recruitment of the WICH complex to replication foci during DNA replication. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. In the WINAC complex, plays an essential role by targeting the complex to acetylated histones, an essential step for VDR-promoter association. -
Tissue specificity
Ubiquitously expressed with high levels of expression in heart, brain, placenta, skeletal muscle and ovary. -
Involvement in disease
Note=BAZ1B is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of BAZ1B may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease. -
Sequence similarities
Belongs to the WAL family. BAZ1B subfamily.
Contains 1 bromo domain.
Contains 1 DDT domain.
Contains 1 PHD-type zinc finger.
Contains 1 WAC domain. -
Developmental stage
Expressed at equal levels in 19-23 weeks old fetal tissues. -
Domain
The N-terminal part (1-345), including the WAC domain and the C motif, mediates the tyrosine-protein kinase activity.
The bromo domain mediates the specific interaction with acetylated histones. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Accumulates in pericentromeric heterochromatin during replication. Targeted to replication foci throughout S phase via its association with PCNA. - Information by UniProt
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Alternative names
- baz1b antibody
- BAZ1B_HUMAN antibody
- Bromodomain adjacent to zinc finger domain protein 1B antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab306266 has not yet been referenced specifically in any publications.