Anti-APG5L/ATG5 antibody (ab228668)
Key features and details
- Rabbit polyclonal to APG5L/ATG5
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-APG5L/ATG5 antibody
See all APG5L/ATG5 primary antibodies -
Description
Rabbit polyclonal to APG5L/ATG5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Pig -
Immunogen
Recombinant fragment within Human APG5L/ATG5 (internal sequence). The exact sequence is proprietary.
Database link: Q9H1Y0 -
Positive control
- ICC/IF HeLa cells and thapsigargin- or chloroquine-treated HepG2 cells. IHC-P: Mouse brain tissue. WB: NT2/D1 and PC-3 whole cell lysate; HeLa cell extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228668 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 32 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 32 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. The ATG12-ATG5 conjugate also negatively regulates the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Also plays a role in translation or delivery of incoming viral RNA to the translation apparatus. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.
May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD. -
Tissue specificity
Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. -
Sequence similarities
Belongs to the ATG5 family. -
Post-translational
modificationsConjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.
Acetylated by EP300. -
Cellular localization
Cytoplasm. Preautophagosomal structure membrane. Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme. - Information by UniProt
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Database links
- Entrez Gene: 532686 Cow
- Entrez Gene: 9474 Human
- Entrez Gene: 11793 Mouse
- Entrez Gene: 641362 Pig
- Omim: 604261 Human
- SwissProt: Q9H1Y0 Human
- SwissProt: Q99J83 Mouse
- SwissProt: Q3MQ04 Pig
see all -
Alternative names
- APG 5 antibody
- APG 5L antibody
- APG5 antibody
see all
Images
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All lanes : Anti-APG5L/ATG5 antibody (ab228668) at 1/1000 dilution
Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 50 µM chloroquine for 24 hours, whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 32 kDa15% SDS-PAGE
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for APG5L/ATG5 (green) using ab228668 (1/1000 dilution) in ICC/IF. Cells mock (left panel) and treated with 50 μM chloroquine for 24 hours (right panel) were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Phalloidin, an F-actin marker (red).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody (ab228668)
Paraffin-embedded mouse brain tissue stained for APG5L/ATG5 with ab228668 at 1/400 dilution in immunohistochemical analysis.
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All lanes : Anti-APG5L/ATG5 antibody (ab228668) at 1/1000 dilution
Lane 1 : NT2/D1 (human embryonal carcinoma cell line) whole cell lysate
Lane 2 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 32 kDa12% SDS-PAGE
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HepG2 (human liver hepatocellular carcinoma cell line) cells stained for APG5L/ATG5 (green) using ab228668 (1/500 dilution) in ICC/IF. Cells were treated with 3 μM thapsigargin 16 hours (right panels) and mock (left panels) and were fixed in ice-cold methanol for 10 minutes, permeabilized with cooled acetone for 2 minutes.
Nuclear counterstain: Hoechst 33342 (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab228668 has been referenced in 17 publications.
- Wan Y et al. PPRV-Induced Autophagy Facilitates Infectious Virus Transmission by the Exosomal Pathway. J Virol 96:e0024422 (2022). PubMed: 35319226
- Zhao W et al. Prototheca bovis induces autophagy in bovine mammary epithelial cells via the HIF-1α and AMPKα/ULK1 pathway. Front Immunol 13:934819 (2022). PubMed: 36148236
- Duarte-Silva E et al. Sildenafil Alleviates Murine Experimental Autoimmune Encephalomyelitis by Triggering Autophagy in the Spinal Cord. Front Immunol 12:671511 (2021). PubMed: 34054847
- Leven AS et al. Association of cell death mechanisms and fibrosis in visceral white adipose tissue with pathological alterations in the liver of morbidly obese patients with NAFLD. Adipocyte 10:558-573 (2021). PubMed: 34743657
- Han M et al. Exosome-transmitted miR-567 reverses trastuzumab resistance by inhibiting ATG5 in breast cancer. Cell Death Dis 11:43 (2020). PubMed: 31969559