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    products/primary-antibodies/apolipoprotein-a-i-antibody-epr19879-30-bsa-and-azide-free-capture-ab242670.pdf

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RecombinantRabMAb

Recombinant Anti-Apolipoprotein A I antibody [EPR19879-30] - BSA and Azide free (Capture) (ab242670)

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Anti-Apolipoprotein A I antibody [EPR19879-30] - BSA and Azide free (Capture) (ab242670)

    Key features and details

    • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
    • Rabbit monoclonal [EPR19879-30] to Apolipoprotein A I - BSA and Azide free (Capture)
    • Suitable for: Sandwich ELISA
    • Reacts with: Mouse

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    Protein
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    Recombinant Human Apolipoprotein A I (ab50239)

    View more associated products

    Overview

    • Product name

      Anti-Apolipoprotein A I antibody [EPR19879-30] - BSA and Azide free (Capture)
      See all Apolipoprotein A I primary antibodies
    • Description

      Rabbit monoclonal [EPR19879-30] to Apolipoprotein A I - BSA and Azide free (Capture)
    • Host species

      Rabbit
    • Tested applications

      Suitable for: Sandwich ELISAmore details
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

    • General notes

      ab242670 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse Apolipoprotein AI. The recommended pair for sandwich ELISA is:
           Capture: ab242670, Mouse Apolipoprotein AI Capture Antibody (unconjugated)
           Detector: ab242915, Mouse Apolipoprotein AI Detector Antibody (unconjugated)
      The reference range value is 156.2 - 10000 pg/ml.

      Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

      This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

      Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

      The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free production
      For more information see here.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      Constituent: 100% PBS
    • Carrier free

      Yes
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Monoclonal
    • Clone number

      EPR19879-30
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipoproteins/Apolipoproteins
      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Other
      • Signal Transduction
      • Metabolism
      • Lipid metabolism
      • Cardiovascular
      • Atherosclerosis
      • Lipoprotein metabolism
      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipoproteins/Apolipoproteins
      • Apolipoproteins
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of lipids and lipoproteins
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Cardiovascular ELISA kits
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipid metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipoprotein metabolism
      • Metabolism
      • Types of disease
      • Heart disease
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Lipoprotein ELISA Kits

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
      • Goat Anti-Rabbit IgG H&L (Biotin) (ab207995)
      • Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)
    • Conjugation kits

      • Alkaline phosphatase Conjugation Kit - Lightning-Link® (ab102850)
      • HRP Conjugation Kit - Lightning-Link® (ab102890)
      • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
    • sELISA pair antibody

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
      • Anti-Apolipoprotein A I antibody [EPR19879-239] - BSA and Azide free (Detector) (ab242915)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab242670 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA
    Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR19879-239] to Apolipoprotein A I - BSA and Azide free (Detector) (ab242915).
    Notes
    Sandwich ELISA
    Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR19879-239] to Apolipoprotein A I - BSA and Azide free (Detector) (ab242915).

    Target

    • Function

      Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
    • Tissue specificity

      Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine.
    • Involvement in disease

      Defects in APOA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). Inheritance is autosomal dominant.
      Defects in APOA1 are a cause of the low HDL levels observed in high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by the absence of plasma HDL, accumulation of cholesteryl esters, premature coronary artery disease, hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness. In HDLD1 patients, ApoA-I fails to associate with HDL probably because of the faulty conversion of pro-ApoA-I molecules into mature chains, either due to a defect in the converting enzyme activity or a specific structural defect in Tangier ApoA-I.
      Defects in APOA1 are the cause of amyloid polyneuropathy-nephropathy Iowa type (AMYLIOWA) [MIM:107680]; also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III. AMYLIOWA is a hereditary generalized amyloidosis due to deposition of amyloid mainly constituted by apolipoprotein A1. The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis. Severe peptic ulcer disease can occurr in some and hearing loss is frequent. Cataracts is present in several, but vitreous opacities are not observed.
      Defects in APOA1 are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
    • Sequence similarities

      Belongs to the apolipoprotein A1/A4/E family.
    • Post-translational
      modifications

      Palmitoylated.
      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P02647 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 11806 Mouse
      • SwissProt: Q00623 Mouse
      • Alternative names

        • Apo-AI antibody
        • ApoA I antibody
        • ApoA-I antibody
        • APOA1 antibody
        • APOA1_HUMAN antibody
        • Apolipoprotein A-I(1-242) antibody
        • Apolipoprotein A1 antibody
        • Apolipoprotein AI antibody
        • Apolipoprotein of high density lipoprotein antibody
        • ApolipoproteinAI antibody
        • Brp14 antibody
        • high density lipoprotein uptake antibody
        • Ltw1 antibody
        • Lvtw1 antibody
        • MGC117399 antibody
        • Sep1 antibody
        • Sep2 antibody
        see all

      Images

      • Anti-Apolipoprotein A I antibody [EPR19879-30] - BSA and Azide free (Capture) (ab242670)
        Anti-Apolipoprotein A I antibody [EPR19879-30] - BSA and Azide free (Capture) (ab242670)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (0)

      Publishing research using ab242670? Please let us know so that we can cite the reference in this datasheet.

      ab242670 has not yet been referenced specifically in any publications.

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