Recombinant Anti-Apolipoprotein B antibody [EPR18817-107] - BSA and Azide free (Capture) (ab252574)
![Sandwich ELISA - Anti-Apolipoprotein B antibody [EPR18817-107] - BSA and Azide free (Capture) (ab252574)](https://www.abcam.com/ps/products/252/ab252574/Images/ab252574-2-human-apolipoprotein-b-antibody-pair-sandwich-elisa-standard-curve-1.jpg "Sandwich ELISA - Anti-Apolipoprotein B antibody [EPR18817-107] - BSA and Azide free (Capture) (ab252574)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18817-107] to Apolipoprotein B - BSA and Azide free (Capture)
- Suitable for: Sandwich ELISA
- Reacts with: Human
Overview
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Product name
Anti-Apolipoprotein B antibody [EPR18817-107] - BSA and Azide free (Capture)
See all Apolipoprotein B primary antibodies -
Description
Rabbit monoclonal [EPR18817-107] to Apolipoprotein B - BSA and Azide free (Capture) -
Host species
Rabbit -
Tested applications
Suitable for: Sandwich ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab252574 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Apolipoprotein B. The recommended pair for sandwich ELISA is:
Capture: ab252574, Human Apolipoprotein B Capture Antibody (unconjugated)
Detector: ab252633, Human Apolipoprotein B Detector Antibody (unconjugated)
The reference range value is 14 - 900 ng/ml.Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18817-107 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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sELISA pair antibody
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab252574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [EPR18817-119] to Apolipoprotein B - BSA and Azide free (Detector) (ab252633).
|
| Notes |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [EPR18817-119] to Apolipoprotein B - BSA and Azide free (Detector) (ab252633). |
Target
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Function
Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. -
Involvement in disease
Hypobetalipoproteinemia, familial, 1
Familial ligand-defective apolipoprotein B-100
Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia. -
Sequence similarities
Contains 1 vitellogenin domain. -
Post-translational
modificationsPalmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle. -
Cellular localization
Cytoplasm. Secreted. - Information by UniProt
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Database links
- Entrez Gene: 338 Human
- Omim: 107730 Human
- SwissProt: P04114 Human
- Unigene: 120759 Human
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Alternative names
- Apo B 100 antibody
- Apo B antibody
- Apo B-100 antibody
see all
Images
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Sandwich ELISA - Anti-Apolipoprotein B antibody [EPR18817-107] - BSA and Azide free (Capture) (ab252574)Representative standard curve from corresponding SimpleStep ELISA® Kit (ab190806).
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![Anti-Apolipoprotein B antibody [EPR18817-107] - BSA and Azide free (Capture) (ab252574)](https://www.abcam.com/ps/products/252/ab252574/Images/ab252574-1-benefits-of-recombinant-antibodies.png)
Anti-Apolipoprotein B antibody [EPR18817-107] - BSA and Azide free (Capture) (ab252574)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab252574 has not yet been referenced specifically in any publications.
