Anti-APPBP2 antibody (ab151305)
Key features and details
- Rabbit polyclonal to APPBP2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-APPBP2 antibody -
Description
Rabbit polyclonal to APPBP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Zebrafish
-
Immunogen
Recombinant fragment, corresponding to a region within amino acids 327-575 of Human APPBP2 (Uniprot ID: Q92624)
-
Positive control
- Jurkat cells lysate, Raji cells; HeLa cells; Ca922 xenograft tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151305 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 67 kDa.
|
|
| ICC/IF |
1/100 - 1/1000.
|
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 67 kDa. |
|
ICC/IF
1/100 - 1/1000. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
May play a role in intracellular protein transport. May be involved in the translocation of APP along microtubules toward the cell surface. -
Sequence similarities
Contains 8 TPR repeats. -
Post-translational
modificationsRapidly degraded by the proteasome upon overexpression of a C-terminal fragment of APP. -
Cellular localization
Nucleus. Cytoplasm > cytoskeleton. Membrane. Associated with membranes and microtubules. - Information by UniProt
-
Database links
- Entrez Gene: 417645 Chicken
- Entrez Gene: 10513 Human
- Entrez Gene: 66884 Mouse
- Entrez Gene: 303396 Rat
- Entrez Gene: 322995 Zebrafish
- Omim: 605324 Human
- SwissProt: Q92624 Human
- SwissProt: Q9DAX9 Mouse
see all -
Alternative names
- amyloid beta precursor protein (cytoplasmic tail) binding protein 2 antibody
- Amyloid beta precursor protein-binding protein 2 antibody
- Amyloid protein binding protein 2 antibody
see all
Images
-
Western blot - Anti-APPBP2 antibody (ab151305)Anti-APPBP2 antibody (ab151305) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 67 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPBP2 antibody (ab151305)Immunohistochemical analysis of paraffin-embedded Ca922 xenograft tissue labeling APPBP2 with ab151305 antibody at 1/100 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-APPBP2 antibody (ab151305)Immunofluorescent analysis of methanol-fixed HeLa cells labeling APPBP2 with ab151305 antibody at 1/100 dilution. Bottom figure represents cells costained with Hoechst 33342.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab151305 has been referenced in 2 publications.
- Wu H et al. A kinesin adapter directly mediates dendritic mRNA localization during neural development in mice. J Biol Chem 295:6605-6628 (2020). PubMed: 32111743
- Gong H et al. APPBP2 enhances non-small cell lung cancer proliferation and invasiveness through regulating PPM1D and SPOP. EBioMedicine 44:138-149 (2019). PubMed: 31105033