Recombinant Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)
![Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)](https://www.abcam.com/ps/products/168/ab168387/Images/ab168387-467546-anti-aquaporin-1-antibody-epr11588-b-westernblot-human.jpg "Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11588(B)] to Aquaporin 1
- Suitable for: WB, IHC-P, Flow Cyt (Intra), ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Aquaporin 1 antibody [EPR11588(B)]
See all Aquaporin 1 primary antibodies -
Description
Rabbit monoclonal [EPR11588(B)] to Aquaporin 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt (Intra), ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human, mouse and rat kidney tissue; ICC/IF: 293T cells; Flow Cyt (Intra): 293T cells. WB: Mouse and rat kidney lysates - boiled and unboiled. Human heart and spleen lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11588(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168387 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/10000. Predicted molecular weight: 28 kDa.
For unpurified use at 1/1000 - 1/10000. |
| IHC-P |
1/8000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For unpurified use at 1/250 - 1/500. |
|
| Flow Cyt (Intra) |
1/2000.
|
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| ICC/IF | (1) |
1/50.
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| Notes |
|---|
|
WB
1/10000. Predicted molecular weight: 28 kDa. For unpurified use at 1/1000 - 1/10000. |
|
IHC-P
1/8000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For unpurified use at 1/250 - 1/500. |
|
Flow Cyt (Intra)
1/2000. |
|
ICC/IF
1/50. |
Target
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Function
Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. -
Tissue specificity
Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver. -
Sequence similarities
Belongs to the MIP/aquaporin (TC 1.A.8) family. -
Domain
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 358 Human
- Entrez Gene: 11826 Mouse
- Entrez Gene: 25240 Rat
- Omim: 107776 Human
- SwissProt: P29972 Human
- SwissProt: Q02013 Mouse
- SwissProt: P29975 Rat
- Unigene: 76152 Human
see all -
Alternative names
- AQP 1 antibody
- AQP CHIP antibody
- AQP-1 antibody
see all
Images
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Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)All lanes : Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387) at 1/10000 dilution (Purified)
Lane 1 : Human heart lysate unboiled
Lane 2 : Human spleen lysate unboiled
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 28 kDaUnglycosylated AQP-1: 28kDa; Glycosylated AQP-1: 35-50kDa
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![Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)](https://www.abcam.com/ps/products/168/ab168387/Images/ab168387-467545-anti-aquaporin-1-antibody-epr11588-b-westernblot-mouse-rat.jpg)
Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)All lanes : Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387) at 1/10000 dilution (Purified)
Lane 1 : Mouse kidney lysate unboiled
Lane 2 : Mouse kidney lysate boiled
Lane 3 : Rat kidney lysate unboiled
Lane 4 : Rat kidney lysate boiled
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 28 kDaUnglycosylated AQP-1: 28kDa; Glycosylated AQP-1: 35-50kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)](https://www.abcam.com/ps/products/168/ab168387/Images/ab168387-13-anti-aquaporin-1-antibody-epr11588-b-immunocytochemistry-293t-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)Immunocytochemistry analysis of 293T (Human embryonic kidney epithelial cell) transfected with EGFP tagged AQP1 construct cells labeling Aquaporin 1 with purified ab168387 at 1/50 dilution (3.9 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with N/A. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)](https://www.abcam.com/ps/products/168/ab168387/Images/ab168387-10-anti-aquaporin-1-antibody-epr11588-b-immunohistochemistry-kidney-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Aquaporin 1 with purified ab168387 at 1/8000 dilution (0.02 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Flow Cytometry (Intracellular) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)](https://www.abcam.com/ps/products/168/ab168387/Images/ab168387-14-anti-aquaporin-1-antibody-epr11588-b-flowcytometry-293t-human.jpg)
Flow Cytometry (Intracellular) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)Flow Cytometry (Intracellular) analysis of 293T (Human embryonic kidney epithelial cell) transfected with EGFP tagged AQP1 construct cells labelling Aquaporin 1 with purified ab168387 at 1/2000 dilution (0.1 µg/mL, Right) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Left). Unlabelled control - /.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)](https://www.abcam.com/ps/products/168/ab168387/Images/ab168387-11-anti-aquaporin-1-antibody-epr11588-b-immunohistochemistry-kidney-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Aquaporin 1 with purified ab168387 at 1/8000 dilution (0.02 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)](https://www.abcam.com/ps/products/168/ab168387/Images/ab168387-12-anti-aquaporin-1-antibody-epr11588-b-immunohistochemistry-kidney-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling Aquaporin 1 with purified ab168387 at 1/8000 dilution (0.02 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)](https://www.abcam.com/ps/products/168/ab168387/Images/ab168387-8-benefits-of-recombinant-antibodies.png)
Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (27)
ab168387 has been referenced in 27 publications.
- Alkhalifa H et al. Inhibition of aquaporins as a potential adjunct to breast cancer cryotherapy. Oncol Lett 21:458 (2021). PubMed: 33907568
- Snuggs JW et al. TonEBP regulates the hyperosmotic expression of aquaporin 1 and 5 in the intervertebral disc. Sci Rep 11:3164 (2021). PubMed: 33542263
- Ryu JY et al. Oscillatory shear stress promotes angiogenic effects in arteriovenous malformations endothelial cells. Mol Med 27:31 (2021). PubMed: 33789563
- Thompson ER et al. Novel delivery of cellular therapy to reduce ischemia reperfusion injury in kidney transplantation. Am J Transplant 21:1402-1414 (2021). PubMed: 32506663
- Kong B et al. MicroRNA-133a-3p inhibits cell proliferation, migration and invasion in colorectal cancer by targeting AQP1. Oncol Lett 22:649 (2021). PubMed: 34386071