Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)
![Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)](https://www.abcam.com/ps/products/57/ab57113/Images/ab57113-368598-anti-argonaute-2-antibody-2e12-1c9-chip-grade-western-blot-MCF7-lysate.jpg "Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)")
Key features and details
- Mouse monoclonal [2E12-1C9] to Argonaute-2 - BSA and Azide free
- Suitable for: WB, ICC/IF, IHC-P, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free
See all Argonaute-2 primary antibodies -
Description
Mouse monoclonal [2E12-1C9] to Argonaute-2 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human, Recombinant fragment
Does not react with: Xenopus laevis -
Immunogen
Recombinant fragment corresponding to Human Argonaute-2 aa 483-859.
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Positive control
- IHC-P: Human stomach. Flow cyt: HeLa cells. WB: HEK-293 cell.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
2E12-1C9 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab57113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (5) |
Use at an assay dependent concentration. Predicted molecular weight: 97 kDa.
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| ICC/IF |
Use at an assay dependent concentration.
|
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| IHC-P | (1) |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 97 kDa. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by EIF2C2/AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also upregulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and upregulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions. -
Sequence similarities
Belongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
Domain
The Piwi domain may perform RNA cleavage by a mechanism similar to that of RNase H. However while RNase H utilizes a triad of Asp-Asp-Glu (DDE) for metal ion coordination, this protein appears to utilize a triad of Asp-Asp-His (DDH). -
Post-translational
modificationsHydroxylated. 4-hydroxylation appears to enhance protein stability but is not required for miRNA-binding or endonuclease activity. -
Cellular localization
Cytoplasm > P-body. Nucleus. Translational repression of mRNAs results in their recruitment to P-bodies. Translocation to the nucleus requires IMP8. - Information by UniProt
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Database links
- Entrez Gene: 27161 Human
- Omim: 606229 Human
- SwissProt: Q9UKV8 Human
- Unigene: 449415 Human
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Alternative names
- Ago 2 antibody
- AGO2_HUMAN antibody
- Argonaute 2 antibody
see all
Images
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Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113) + MCF7 cell lysate
Predicted band size: 97 kDa
Observed band size: 97 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)](https://www.abcam.com/ps/products/57/ab57113/Images/ab57113-261138-anti-argonaute-2-antibody-2e12-1c9-chip-grade-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)Ago2 / eIF2C2 antibody (ab57113) used in immunofluorescence at 10ug/ml on HeLa cells.
This image was generated using the ascites version of the product.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)](https://www.abcam.com/ps/products/57/ab57113/Images/ab57113-261137-anti-argonaute-2-antibody-2e12-1c9-chip-grade-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)Ago2 / eIF2C2 antibody (ab57113) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human stomach.
This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)](https://www.abcam.com/ps/products/57/ab57113/Images/ab57113-70644-anti-argonaute-2-antibody-2e12-1c9-chip-grade-flow-cytometry.jpg)
Flow Cytometry - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)Overlay histogram showing HeLa cells stained with ab57113 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57113, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.
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![Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)](https://www.abcam.com/ps/products/57/ab57113/Images/ab57113-334180-anti-argonaute-2-antibody-2e12-1c9-chip-grade-western-blot.jpg)
Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)All lanes : Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113) at 5 µg/ml
Lane 1 : Argonaute-2 overexpressing HEK-293 cells line
Lane 2 : Argonaute-2 overexpressing HEK-293 cell line cotransfected with Argonaute-2 validated chimera RNAi.
Predicted band size: 97 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?The band at about 42 kDa corresponds to the recombinant fragment of human Argonaute-2 aa 483-859 (377 aa length).
The loading control is GAPDH.
This image was generated using the ascites version of the product.
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![Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)](https://www.abcam.com/ps/products/57/ab57113/Images/ab57113-70645-anti-argonaute-2-antibody-2e12-1c9-chip-grade-western-blot.jpg)
Western blot - Anti-Argonaute-2 antibody [2E12-1C9] - BSA and Azide free (ab57113)Western Blot detection against the recombinant fragment imunogen (68 KDa for a.a. ~377 plus GST tag +26 kDa).
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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Datasheet download
References (150)
ab57113 has been referenced in 150 publications.
- Zhao X et al. N6-methyladenosine-dependent modification of circGARS acts as a new player that promotes SLE progression through the NF-κB/A20 axis. Arthritis Res Ther 24:37 (2022). PubMed: 35120571
- Ohno SI et al. Nuclear microRNAs release paused Pol II via the DDX21-CDK9 complex. Cell Rep 39:110673 (2022). PubMed: 35417682
- Qian CJ et al. Circ_0000705 facilitates proline metabolism of esophageal squamous cell carcinoma cells by targeting miR-621/PYCR1 axis. Discov Oncol 13:50 (2022). PubMed: 35731336
- Willkomm S et al. Single-molecule FRET uncovers hidden conformations and dynamics of human Argonaute 2. Nat Commun 13:3825 (2022). PubMed: 35780145
- Zhao X et al. Circular RNA circLOC101928570 suppresses systemic lupus erythematosus progression by targeting the miR-150-5p/c-myb axis. J Transl Med 20:547 (2022). PubMed: 36435781
