Recombinant Anti-Argonaute-2 antibody [EPR10410] (ab156870)
![Western blot - Anti-Argonaute-2 antibody [EPR10410] (ab156870)](https://www.abcam.com/ps/products/156/ab156870/Images/ab156870-513189-anti-argonaute-2-antibody-epr10410-western-blot-wildtype-hct-116-pools-ago2-knockout-hct-116-u87-mg-mcf7.jpg "Western blot - Anti-Argonaute-2 antibody [EPR10410] (ab156870)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10410] to Argonaute-2
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Argonaute-2 antibody [EPR10410]
See all Argonaute-2 primary antibodies -
Description
Rabbit monoclonal [EPR10410] to Argonaute-2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Argonaute-2 aa 1-100. The exact sequence is proprietary.
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Positive control
- WB: HeLa, MCF7, HepG2 and K562 cell lysates. Rat liver and mouse liver lysates. IHC-P: Human breast carcinoma and human kidney tissues. Flow Cyt (intra): HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10410 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/200.
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| WB |
1/1000 - 1/10000. Predicted molecular weight: 97 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples. |
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| ICC/IF |
1/100 - 1/250.
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| Notes |
|---|
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Flow Cyt (Intra)
1/200. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 97 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples. |
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ICC/IF
1/100 - 1/250. |
Target
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Function
Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by EIF2C2/AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also upregulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and upregulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions. -
Sequence similarities
Belongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
Domain
The Piwi domain may perform RNA cleavage by a mechanism similar to that of RNase H. However while RNase H utilizes a triad of Asp-Asp-Glu (DDE) for metal ion coordination, this protein appears to utilize a triad of Asp-Asp-His (DDH). -
Post-translational
modificationsHydroxylated. 4-hydroxylation appears to enhance protein stability but is not required for miRNA-binding or endonuclease activity. -
Cellular localization
Cytoplasm > P-body. Nucleus. Translational repression of mRNAs results in their recruitment to P-bodies. Translocation to the nucleus requires IMP8. - Information by UniProt
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Database links
- Entrez Gene: 27161 Human
- Entrez Gene: 239528 Mouse
- Entrez Gene: 59117 Rat
- Omim: 606229 Human
- SwissProt: Q9UKV8 Human
- SwissProt: Q8CJG0 Mouse
- SwissProt: Q9QZ81 Rat
- Unigene: 449415 Human
see all -
Alternative names
- Ago 2 antibody
- AGO2_HUMAN antibody
- Argonaute 2 antibody
see all
Images
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Western blot - Anti-Argonaute-2 antibody [EPR10410] (ab156870)All lanes : Anti-Argonaute-2 antibody [EPR10410] (ab156870) at 1/1000 dilution
Lane 1 : Wild-type HCT 116 cell lysate
Lane 2 : AGO2 knockout HCT 116 cell lysate
Lane 3 : U-87 MG cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-Argonaute-2 antibody [EPR10410] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab156870 was shown to bind specifically to Argonaute-2. A band was observed at 95 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in AGO2 knockout cell line. To generate this image, wild-type and AGO2 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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![Western blot - Anti-Argonaute-2 antibody [EPR10410] (ab156870)](https://www.abcam.com/ps/products/156/ab156870/Images/ab156870-284139-anti-argonaute-2-antibody-epr10410-western-blot.jpg)
Western blot - Anti-Argonaute-2 antibody [EPR10410] (ab156870)All lanes : Anti-Argonaute-2 antibody [EPR10410] (ab156870) at 1/5000 dilution (purified)
Lane 1 : Rat liver lysates
Lane 2 : Mouse kidney lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 97 kDaBlocking and diluting buffer: 5% NFDM/TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10410] (ab156870)](https://www.abcam.com/ps/products/156/ab156870/Images/ab156870-284071-anti-argonaute-2-antibody-epr10410-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10410] (ab156870)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human ovarian carcinoma tissue sections labeling Argonaute -2 with purified ab156870 at 1:100 dilution (1.9 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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![Western blot - Anti-Argonaute-2 antibody [EPR10410] (ab156870)](https://www.abcam.com/ps/products/156/ab156870/Images/ab156870-284135-anti-argonaute-2-antibody-epr10410-western-blot.jpg)
Western blot - Anti-Argonaute-2 antibody [EPR10410] (ab156870)All lanes : Anti-Argonaute-2 antibody [EPR10410] (ab156870) at 1/1000 dilution (purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HUVEC (Human umbilical vein endothelial cell) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 97 kDaBlocking and diluting buffer: 5% NFDM/TBST
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![Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [EPR10410] (ab156870)](https://www.abcam.com/ps/products/156/ab156870/Images/ab156870-284088-anti-argonaute-2-antibody-epr10410-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Argonaute-2 antibody [EPR10410] (ab156870)Immunocytochemistry/ Immunofluorescence analysis of MCF-7 (Human breast adenocarcinoma epithelial cell) cells labeling Argonaute-2 with Purified ab156870 at 1:200 dilution (9.5μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10410] (ab156870)](https://www.abcam.com/ps/products/156/ab156870/Images/ab156870-164047-anti-argonaute-2-antibody-epr10410-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10410] (ab156870)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Ago2 / eIF2C2 with unpurified ab156870 at 1/50 dilution.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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![Western blot - Anti-Argonaute-2 antibody [EPR10410] (ab156870)](https://www.abcam.com/ps/products/156/ab156870/Images/ab156870-164049-anti-argonaute-2-antibody-epr10410-western-blot.jpg)
Western blot - Anti-Argonaute-2 antibody [EPR10410] (ab156870)All lanes : Anti-Argonaute-2 antibody [EPR10410] (ab156870) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 97 kDa -
![Flow Cytometry (Intracellular) - Anti-Argonaute-2 antibody [EPR10410] (ab156870)](https://www.abcam.com/ps/products/156/ab156870/Images/ab156870-280397-anti-argonaute-2-antibody-epr10410-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Argonaute-2 antibody [EPR10410] (ab156870)Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Argonaute-2 (red) with unpurifiedab156870 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10410] (ab156870)](https://www.abcam.com/ps/products/156/ab156870/Images/ab156870-164048-anti-argonaute-2-antibody-epr10410-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10410] (ab156870)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Ago2 / eIF2C2 with unpurified ab156870 at 1/50 dilution.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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![Anti-Argonaute-2 antibody [EPR10410] (ab156870)](https://www.abcam.com/ps/products/156/ab156870/Images/ab156870-9-benefits-of-recombinant-antibodies.png)
Anti-Argonaute-2 antibody [EPR10410] (ab156870)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab156870 has been referenced in 12 publications.
- Yu J et al. LINC01234 Accelerates the Progression of Breast Cancer via the miR-525-5p/Cold Shock Domain-Containing E1 Axis. Dis Markers 2022:6899777 (2022). PubMed: 35923244
- Vujovic F et al. The fate of notch-1 transcript is linked to cell cycle dynamics by activity of a natural antisense transcript. Nucleic Acids Res 49:10419-10430 (2021). PubMed: 34520549
- Yin M et al. Reduced SULT2B1b expression alleviates ox-LDL-induced inflammation by upregulating miR-148-3P via inhibiting the IKKß/NF-?B pathway in macrophages. Aging (Albany NY) 13:3428-3442 (2021). PubMed: 33428590
- Mi L et al. Circ_0000144 functions as a miR-623 sponge to enhance gastric cancer progression via up-regulating GPRC5A. Biosci Rep 40:N/A (2020). PubMed: 32766708
- Huo C et al. Defective Viral Particles Produced in Mast Cells Can Effectively Fight Against Lethal Influenza A Virus. Front Microbiol 11:553274 (2020). PubMed: 33250863